Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (6): 797-803.DOI: 10.11983/CBB19107
• TEACHING FORUM • Previous Articles
Xifeng Chen,Yaping Liu,Bojun Ma()
Received:
2019-06-11
Accepted:
2019-09-17
Online:
2019-11-01
Published:
2020-07-09
Contact:
Bojun Ma
Xifeng Chen,Yaping Liu,Bojun Ma. Design and Practice of a New Teaching Project of the Map-based Cloning Experiment in Genetics[J]. Chinese Bulletin of Botany, 2019, 54(6): 797-803.
Figure 1 Recombination patterns between the target gene and the molecular markers during meiosis A and a represent a pair of dominant and recessive alleles, of which AA and Aa show dominant phenotypes and aa shows a recessive phenotype; M and m represent a given molecular marker at the same locus derived from the dominant parent (AA) and the recessive parent (aa), respectively.
Figure 2 The phenotype of the rice spl5 lesion mimic mutant and a schematic workflow of generation of the genetic mapping population (A) The plant phenotype of wild-type control Nipponbare, a Japonica rice cultivar, and the spl5 mutant; (B) The leaf phenotype of wild-type control and the spl5 mutant; (C) A workflow showing the generation of an F2 mapping population by crossing an Indica cultivar 9311 with the spl5 mutant.
SSR标记 | 正向引物 (5°-3°) | 反向引物 (5°-3°) |
---|---|---|
RM21103 | ACAAGTCCACAAGGACCACAACC | TGCTCCACCCAAAGATACAGAGC |
RM21309 | GAGTGGATTTACTGACAAGCTCTTCC | GATTCTCTTGGAGCCCATACTCC |
RM21470 | TCTTGCCATCACATAGCAACAGG | ACTCGGTGAGCATCCAATGTCC |
RM22426 | ATTTCGTCGTCGTGTCTGTCC | TTAAAGCAGCCTGATCGAAAGC |
RM22648 | AGCAGCTCCGATTGTGTTAGCC | AATGGCGTCTGTGGTACTTTGC |
RM22925 | CGATTACCAGGACAACAGATCACG | GTGGCTCTTGAGTGTACGTGTGC |
Table 1 PCR primers of the SSR markers used in this study
SSR标记 | 正向引物 (5°-3°) | 反向引物 (5°-3°) |
---|---|---|
RM21103 | ACAAGTCCACAAGGACCACAACC | TGCTCCACCCAAAGATACAGAGC |
RM21309 | GAGTGGATTTACTGACAAGCTCTTCC | GATTCTCTTGGAGCCCATACTCC |
RM21470 | TCTTGCCATCACATAGCAACAGG | ACTCGGTGAGCATCCAATGTCC |
RM22426 | ATTTCGTCGTCGTGTCTGTCC | TTAAAGCAGCCTGATCGAAAGC |
RM22648 | AGCAGCTCCGATTGTGTTAGCC | AATGGCGTCTGTGGTACTTTGC |
RM22925 | CGATTACCAGGACAACAGATCACG | GTGGCTCTTGAGTGTACGTGTGC |
Figure 3 Linkage analysis and genetic mapping of the SPL5 gene (A) A physical map of the SSR markers on chromosomes used in this study; (B) Analysis of the PCR products of the SSR markers by electrophoresis; (C) A genetic linkage map of the SPL5 gene.
分子标记 | 20个F2突变型单株中各基因型的数量 | χ2值 | P值 | ||
---|---|---|---|---|---|
MM | Mm | mm | |||
RM21103 | 0 | 0 | 20 | 60 | <0.01 |
RM21309 | 0 | 3 | 17 | 38.7 | <0.01 |
RM21470 | 1 | 7 | 12 | 13.9 | <0.01 |
RM22426 | 4 | 11 | 5 | 0.3 | >0.05 |
RM22648 | 5 | 9 | 6 | 0.3 | >0.05 |
RM22925 | 7 | 10 | 3 | 1.6 | >0.05 |
Table 2 The Chi-square (χ2) test for the genotype separation ratio of the SSR markers in this study
分子标记 | 20个F2突变型单株中各基因型的数量 | χ2值 | P值 | ||
---|---|---|---|---|---|
MM | Mm | mm | |||
RM21103 | 0 | 0 | 20 | 60 | <0.01 |
RM21309 | 0 | 3 | 17 | 38.7 | <0.01 |
RM21470 | 1 | 7 | 12 | 13.9 | <0.01 |
RM22426 | 4 | 11 | 5 | 0.3 | >0.05 |
RM22648 | 5 | 9 | 6 | 0.3 | >0.05 |
RM22925 | 7 | 10 | 3 | 1.6 | >0.05 |
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