Priming Effect of Abscisic Acid on High Temperature Stress During Rice Heading-flowering Stage

doi:10.11983/CBB22022

Abstract

Abstract: To understand the priming effect of abscisic acid (ABA) on high temperature stress during rice heading-flowering stage, Huanghuazhan, a main rice variety in Jiangxi province, was used as experimental material. Six kinds of solutions were arranged to spray on rice at the booting stage, including distilled water, ABA solution (10, 50 and 100 µmol∙L^-1), fluridone (FLU) and proanthocyanidins (PC), then the rice plants were moved to control (CK) and high temperature stress (HS) conditions for 8 d. The effects of ABA by spraying at booting stage were measured by the accumulation of reactive oxygen species (ROS), antioxidant defense capacity, yield components and the expression levels of related genes. The results showed that significant negative correlations were detected between the panicle length, panicle weight, percentage of filled spikelets, 1 000-kernel weight, grain yield and contents of superoxide anion and hydrogen peroxide respectively. Spraying ABA solution significantly upregulated the expression of ABA-responsive and antioxidant defense genes, and decreased contents of ROS by 8.24%-31.35% under high temperature stress. The panicle length, panicle weight, percentage of filled spikelets and 1 000-kernel weight were significantly increased by spraying ABA solution, as well as the expression levels of yield related genes. The grain yield was increased by 12.73%-20.77% by spraying ABA solution under high temperature stress. The application of the ABA biosynthesis inhibitor, FLU, inhibited the ABA biosynthesis and leaded to excessive accumulation of ROS and yield loss under high temperature stress. While application of the antioxidant PC inhibited the excessive accumulation of ROS and improved grain yield under high temperature stress. These results suggested that spraying ABA solution at booting stage enhanced tolerance to high temperature stress in rice at heading-flowering stage by upregulating not only ABA signaling pathway, but also antioxidant defense activity and yield related genes, so as to further improve grain yield under high temperature stress.

Key words: rice (Oryza sativa), abscisic acid, high temperature stress, reactive oxygen species, yield

Liu Xiaolong, Ji Ping, Yang Hongtao, Ding Yongdian, Fu Jialing, Liang Jiangxia, Yu Congcong. Priming Effect of Abscisic Acid on High Temperature Stress During Rice Heading-flowering Stage[J]. Chinese Bulletin of Botany, 2022, 57(5): 596-610.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22022

https://www.chinbullbotany.com/EN/Y2022/V57/I5/596

Figures/Tables 9

References 51

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Primer name	Primer sequence (5'-3')
SalTF	CGAAATAATGTTCCATGGTGTT
SalTR	TGTACTACGGATCGGTGCAA
OsWsi18F	TGTGACTCGATCCAGCGTAG
OsWsi18R	GTTCCTGCTGAGAAGCCATC
OsACT1F	TTCCAGCCTTCCTTCATA
OsACT1R	AACGATGTTGCCATATAGAT
OsCATBF	GCTGGTGAGAGATACCGGTCA
OsCATBR	TCAACCCACCGCTGGAGA
OsAPX6F	CCCCAAGATCCCCATGATCTA
OsAPX6R	CCTCTGGCGGGCATTG
OsFe-SODF	CGACGCCGAGGAATTTCTAG
OsFe-SODR	AGGTGGTGTAAGTGTCTCTCATGC
OsCu/Zn-SODF	TGTGACGGGACTTACTCCTGG
OsCu/Zn-SODR	CACCCATTCGTAGTATCGCCA
Ghd7F	AATCCGGTACGCGTCCAGA
Ghd7R	CCAAGCTCAAGCCTACTAGG
WxF	TTGGGATACCAGCGTTGTGG
WxR	CGGTCTTTCCCCAAACCTTCT
OsAGPL2F	AAGCCGAGAGCGTGGTAAAA
OsAGPL2R	CAGGAGCGCTAGGGTTTTCA
OsAGPL3F	TGCCGATGAGCAACTGCATA
OsAGPL3R	TTATACGCCCGGGAAAGGTG

Primer name	Primer sequence (5'-3')
SalTF	CGAAATAATGTTCCATGGTGTT
SalTR	TGTACTACGGATCGGTGCAA
OsWsi18F	TGTGACTCGATCCAGCGTAG
OsWsi18R	GTTCCTGCTGAGAAGCCATC
OsACT1F	TTCCAGCCTTCCTTCATA
OsACT1R	AACGATGTTGCCATATAGAT
OsCATBF	GCTGGTGAGAGATACCGGTCA
OsCATBR	TCAACCCACCGCTGGAGA
OsAPX6F	CCCCAAGATCCCCATGATCTA
OsAPX6R	CCTCTGGCGGGCATTG
OsFe-SODF	CGACGCCGAGGAATTTCTAG
OsFe-SODR	AGGTGGTGTAAGTGTCTCTCATGC
OsCu/Zn-SODF	TGTGACGGGACTTACTCCTGG
OsCu/Zn-SODR	CACCCATTCGTAGTATCGCCA
Ghd7F	AATCCGGTACGCGTCCAGA
Ghd7R	CCAAGCTCAAGCCTACTAGG
WxF	TTGGGATACCAGCGTTGTGG
WxR	CGGTCTTTCCCCAAACCTTCT
OsAGPL2F	AAGCCGAGAGCGTGGTAAAA
OsAGPL2R	CAGGAGCGCTAGGGTTTTCA
OsAGPL3F	TGCCGATGAGCAACTGCATA
OsAGPL3R	TTATACGCCCGGGAAAGGTG

Treatments		Shoot length (cm)	Stem dry weight (g)	Panicle length (cm)	Panicle weight (g)
CK	-ABA	85.54±5.46 a	37.27±5.10 a	20.96±0.92 b	3.01±0.18 ab
	ABA1	86.70±5.38 a	38.19±3.92 a	21.41±0.94 ab	3.05±0.38 ab
	ABA2	86.54±3.47 a	39.90±4.39 a	21.64±0.90 ab	3.03±0.41 ab
	ABA3	87.88±3.74 a	41.52±2.66 a	21.79±0.61 a	3.12±0.42 a
	FLU	84.10±4.24 a	37.28±5.77 a	20.92±0.67 b	2.75±0.29 b
	PC	87.30±5.50 a	41.61±2.77 a	22.03±0.73 a	3.09±0.18 ab
HS	-ABA	82.64±3.23 a	34.79±4.08 a	19.96±0.86 b	2.42±0.29 b
	ABA1	85.01±6.15 a	36.08±3.69 a	20.42±0.66 ab	2.73±0.32 a
	ABA2	85.09±6.20 a	37.74±5.74 a	20.94±1.96 ab	2.86±0.21 a
	ABA3	87.02±3.37 a	38.55±5.34 a	20.85±0.44 ab	2.81±0.23 a
	FLU	83.00±4.00 a	36.96±3.34 a	19.84±0.73 b	2.36±0.19 b
	PC	85.03±3.98 a	37.56±2.99 a	21.28±1.03 a	2.89±0.40 a

Treatments		Shoot length (cm)	Stem dry weight (g)	Panicle length (cm)	Panicle weight (g)
CK	-ABA	85.54±5.46 a	37.27±5.10 a	20.96±0.92 b	3.01±0.18 ab
	ABA1	86.70±5.38 a	38.19±3.92 a	21.41±0.94 ab	3.05±0.38 ab
	ABA2	86.54±3.47 a	39.90±4.39 a	21.64±0.90 ab	3.03±0.41 ab
	ABA3	87.88±3.74 a	41.52±2.66 a	21.79±0.61 a	3.12±0.42 a
	FLU	84.10±4.24 a	37.28±5.77 a	20.92±0.67 b	2.75±0.29 b
	PC	87.30±5.50 a	41.61±2.77 a	22.03±0.73 a	3.09±0.18 ab
HS	-ABA	82.64±3.23 a	34.79±4.08 a	19.96±0.86 b	2.42±0.29 b
	ABA1	85.01±6.15 a	36.08±3.69 a	20.42±0.66 ab	2.73±0.32 a
	ABA2	85.09±6.20 a	37.74±5.74 a	20.94±1.96 ab	2.86±0.21 a
	ABA3	87.02±3.37 a	38.55±5.34 a	20.85±0.44 ab	2.81±0.23 a
	FLU	83.00±4.00 a	36.96±3.34 a	19.84±0.73 b	2.36±0.19 b
	PC	85.03±3.98 a	37.56±2.99 a	21.28±1.03 a	2.89±0.40 a

Treatments		Panicle number	Spikelets per panicle	Percentage of filled spikelets (%)	1000-kernel weight (g)	Harvest index (%)	Grain yield per hole (g)
CK	-ABA	14.22±1.86 a	124.73±14.91 a	84.89±2.11 a	22.97±1.21 a	49.41±1.79 a	34.31±4.49 a
	ABA1	14.44±1.74 a	121.55±12.92 a	85.21±1.76 a	22.80±1.45 a	48.77±1.99 a	33.90±4.47 a
	ABA2	14.78±1.64 a	124.81±13.85 a	84.63±2.78 a	22.43±1.06 a	48.39±1.72 a	34.73±3.50 a
	ABA3	15.33±1.32 a	128.67±13.16 a	84.00±2.63 a	21.85±0.91 a	48.53±1.23 a	35.92±1.81 a
	FLU	14.44±1.42 a	123.27±14.02 a	83.02±3.71 a	22.61±0.91 a	49.43±3.72 a	33.31±4.41 a
	PC	15.78±1.56 a	124.53±12.76 a	85.26±2.58 a	21.93±0.58 a	48.58±2.21 a	36.46±2.65 a
HS	-ABA	13.56±1.67 a	119.76±16.90 a	78.02±1.96 b	19.76±0.54 c	44.83±2.73 a	24.84±3.69 bc
	ABA1	14.22±1.39 a	119.04±11.58 a	81.93±1.63 a	20.72±0.81 abc	46.70±2.03 a	28.59±2.84 a
	ABA2	14.56±1.88 a	121.99±8.02 a	81.12±2.48 a	20.98±1.45 a	47.04±2.78 a	30.00±3.14 a
	ABA3	14.44±2.07 a	115.09±18.40 a	81.10±3.45 a	21.19±0.93 a	44.76±3.97 a	28.01±2.41 ab
	FLU	14.22±1.99 a	113.38±16.50 a	75.43±3.33 b	19.81±0.50 bc	42.69±2.39 a	23.74±2.29 c
	PC	14.78±1.30 a	116.31±14.12 a	81.89±4.24 a	20.79±1.32 ab	46.01±3.50 a	29.08±3.47 a