Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (3): 320-326.DOI: 10.11983/CBB22043
• TECHNIQUES AND METHODS • Previous Articles Next Articles
Haitao Hu, Tingting Qian, Ling Yang()
Received:
2022-03-10
Accepted:
2022-05-11
Online:
2022-05-01
Published:
2022-05-18
Contact:
Ling Yang
Haitao Hu, Tingting Qian, Ling Yang. Detection of Reactive Oxygen Species Using H2DCFDA Probe in Plant[J]. Chinese Bulletin of Botany, 2022, 57(3): 320-326.
Figure 1 Reactive oxygen species (ROS) evaluation in rice protoplasts using 2′,7′-dichlorofluorescein (DCF)-based flow cytometry (A) ROS fluorescence intensity in rice leaves under normal growth conditions; (B) ROS fluorescence intensity in rice leaves under PEG-8000 treatment conditions; (C) The relative fluorescence intensity in treatment and control groups was determined to assess the ROS content. n=3; ** indicates significant difference at P<0.01 level by Student’s t test. SSC: Side scatter
Figure 2 Confocal imaging analysis of rice leaves under normal growth (A)-(D) and PEG-8000 treatment (E)-(H) Red is the spontaneous fluorescence of chlorophyll, and green is the fluorescence of 2′,7′-dichlorofluorescein (DCF) generated by 2′,7′-dichlorodi-hydrofluorescein diacetate (H2DCFDA) oxidation. Bars=100 µm
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