Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (3): 327-339.DOI: 10.11983/CBB21192

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment and Application of a Rapid Genetic Transformation Method for Peanut

Qiong Zhai, Rongqin Chen, Xiaohua Liang, Chuchun Zeng, Bo Hu, Ling Li, Xiaoyun Li()   

  1. Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China
  • Received:2021-11-13 Accepted:2022-03-03 Online:2022-05-01 Published:2022-05-18
  • Contact: Xiaoyun Li
  • About author:First author contact: These authors contributed equally to this paper

Abstract: Plant transformation is an important tool for genetic engineering. The key technology of genetic transformation is to introduce foreign genes into plants genomes quickly and efficiently and reduce the time to obtain transgenic offspring. Peanut (Arachis hypogaea) is an important oil and cash crop in China. The genetic transformation system is still not well established in peanuts. It seriously restricts the function research of peanut genes and the molecular breeding progress. Here, we established a rapid and stable genetic transformation system in peanuts. The Agrobacterium tumefaciens was injected into the second stem of peanut to produce transgenic plants. Then positive transgenic peanut was transplanted and tamped backfill to cover the injection point. Those seeds above the injection point were picked for further screening and analyzing. The results showed that over 40% of transgenic plants were obtained and displayed chimeric in T0 generation. The T0 seeds were harvested about 5 months after rapid-transformation. About 9% of T1 peanuts were hybrids rather than chimeras. To solve the problem of few seed in some transgenic plants, the rapid-transformation system was combined with traditional tissue culture. This rapid-transformation system has potential value in garlic (Allium sativum), potato (Solanum tuberosum), and freesia (Freesia refracta). Altogether, this study establishes a rapid and stable genetic transformation system for peanuts, which sheds light on other plants’ genetic transformation.

Key words: rapid transformation, Arachis hypogaea, Allium sativum, Solanum tuberosum, Freesia refracta