Chinese Bulletin of Botany ›› 2021, Vol. 56 ›› Issue (1): 56-61.DOI: 10.11983/CBB20095

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed

Zhengjun Xia1,*(), Yuzhuo Li1,2, Jinlong Zhu1, Hongyan Wu1, Kun Xu1, Hong Zhai1   

  1. 1Northeast Institute of Geography and Agroecology, the Innovative Academy of Seed Design, Chinese Academy of Sciences, Harbin 150081, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2020-05-26 Accepted:2020-10-05 Online:2021-01-01 Published:2021-01-15
  • Contact: Zhengjun Xia

Abstract: Establishment of a simple, quick, non-destructive, and continuous sampling procedure, can save planting cost and accelerate gene functional analysis and breeding procedures. In this study, we created a rapid, non-destructive, and continuous sampling technique using micro electric driller and air pump. We also optimized the high throughput DNA extraction method for the 384 deep-well plate and genotyping method. Furthermore, this technique could be applied in rice, maize and other crops for seed sampling and high throughput genotyping.

Key words: soybean (Glycine max), seed, drilling, continuing sampling, 384 deep-well plate, genotyping