%A Zhengjun Xia, Yuzhuo Li, Jinlong Zhu, Hongyan Wu, Kun Xu, Hong Zhai %T A Rapid, Non-destructive and Continuous Sampling Technique and DNA Extraction for Soybean Seed %0 Journal Article %D 2021 %J Chinese Bulletin of Botany %R 10.11983/CBB20095 %P 56-61 %V 56 %N 1 %U {https://www.chinbullbotany.com/CN/abstract/article_61004.shtml} %8 2021-01-01 %X

Establishment of a simple, quick, non-destructive, and continuous sampling procedure, can save planting cost and accelerate gene functional analysis and breeding procedures. In this study, we created a rapid, non-destructive, and continuous sampling technique using micro electric driller and air pump. We also optimized the high throughput DNA extraction method for the 384 deep-well plate and genotyping method. Furthermore, this technique could be applied in rice, maize and other crops for seed sampling and high throughput genotyping.