Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (5): 615-621.DOI: 10.11983/CBB16219

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Effect of Different Elicitors on Cell Growth and Polysaccharides Content of Achyranthes bidentata

Ping Li1,2, Yan Xu1, Xiaoli Zhang1,3, Jiong Li4, Xiting Zhao1,3, Mingjun Li1,3*   

  1. 1College of Life Sciences, Henan Normal University, Xinxiang 453007, China
    2Zhengzhou Shuqing Medical College, Zhengzhou 450064, China
    3Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs, University of Henan Province, Henan Province Engineering Laboratory of Green Medicinal Plant Biotechnology, Xinxiang 453007, China
    4Chinese Medical Materials Manufacturing Technology Center, Department of Agriculture of Henan Province, Zhengzhou 450008, China
  • Received:2016-11-14 Accepted:2017-03-06 Online:2017-09-01 Published:2017-07-10
  • Contact: Mingjun Li


To enhance the polysaccharides content of Achyranthes bidentata suspension cell, yeast extract, Pleurotus citrinopileatus and salicylic acid were added at different concentrations or at different times as the elicitor. With 2.5% (v/v) yeast elicitor added at 12 days, the cell dry weight was the greatest, reaching 46.75 g·L-1; polysaccharides content was less, 5.76 mg·g-1. With 5 mgGE·L-1 fungal elicitor added after culture for 9 days, polysaccharides content could reach 6.56 mg·g-1; with cell dry weight 28.3 g·L-1. With 1 mg·g-1 SA elicitation, the effect was a little inferior to the above two effects.

Key words: Achyranthes bidentata, cell suspension culture, elicitor, polysaccharides