Chinese Bulletin of Botany ›› 2015, Vol. 50 ›› Issue (1): 1-11.DOI: 10.3724/SP.J.1259.2015.00001


Dynamics and Interaction of Ca2+ and Nitric Oxide in Wheat Suspension Cells in the Hypersensitive Response

Mei Qiao, Jiawei Sun, Yan Chen, Shengfang Han, Chunyan Hou, Gang Liu*, Dongmei Wang*   

  1. College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China
  • Received:2013-10-30 Accepted:2014-02-23 Online:2015-01-01 Published:2015-04-09
  • Contact: Liu Gang,Wang Dongmei
  • About author:

    ? These authors contributed equally to this paper

Abstract: Suspension cells of wheat varieties Lovrin 10 and Zhengzhou 5389 were stimulated with IWF-260, a leaf intercellular washing fluid induced by leaf rust. The dynamics and interaction of Ca2+ and nitric oxide (NO) in the hypersensitive response induced by IWF-260 were analysed. The fluorescence molecular probe Fluo-3AM and DAF-FM DA were used to mark Ca2+ and NO, respectively. The two kinds of suspension cells showed differences in the concentration of Ca2+. Lovrin 10, a disease-resistant variety, showed two peaks of [Ca2+]cyt, at 330 and 700 s, on stimulation, whereas Zhengzhou 5389, a susceptible variety, showed no obvious change in [Ca2+]cyt with stimulation. The increased [Ca2+]cyt depended on Ca2+ flowing into cells, which suggests that Ca2+ may be involved in the hypersensitive response. Like Ca2+, NO showed a similar pattern after elicitor stimulation. For Lovrin 10, NO showed a peak, with no change for Zhengzhou 5389. Thus, NO production and extracellular calcium influx are closely related to wheat suspension cells’ response to stimulation; NO may play a role downstream of calcium.