Chinese Bulletin of Botany ›› 2015, Vol. 50 ›› Issue (1): 12-21.DOI: 10.3724/SP.J.1259.2015.00012

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Preliminary Functional Analysis of Microtubule-associated Protein GMAP65-1 from Gerbera hybrida

Lingfei Li, Jianzong Peng, Xiaojing Wang*   

  1. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China
  • Received:2014-03-14 Revised:2014-11-01 Online:2015-01-01 Published:2015-04-09

Abstract: Microtubule-associated proteins play vital roles in plant growth and development. We cloned a full cDNA encoding microtubule-associated protein GMAP65-1 by RT-PCR and RACE techniques and characterized the clone. The complete cDNA (1 883 bp) contains a 1 740-bp open reading frame encoding 579 amino acid residues. The gene expression of GMAP65-1 was higher in young roots, leaves and ray florets. Moreover, its expression was induced by gibberellin. Overexpression of GMAP65-1 in transgenic Arabidopsis enlarged the area of leaf and petal. GMAP65-1 might be involved in leaf and petal morphogenesis. This study contributes to providing the theoretical basis and gene resources for flower molecular breeding of Gerbera.