Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (5): 608-614.DOI: 10.11983/CBB16196
• TECHNIQUES AND METHODS • Previous Articles Next Articles
Hongwei Yao1, Yang Liu2, Yulai Cheng2, Haiyang Yu3, Zhiliang Liu3, Ju Yang1
Received:
2016-10-10
Accepted:
2017-03-07
Online:
2017-09-01
Published:
2017-07-10
Contact:
Ju Yang
Hongwei Yao, Yang Liu, Yulai Cheng, Haiyang Yu, Zhiliang Liu, Ju Yang. Fluorescence Imaging of the Extracellular Zinc Distribution in Plants by Using a Highly Specific Fluorescent Probe[J]. Chinese Bulletin of Botany, 2017, 52(5): 608-614.
Figure 2 Specific and sensitivity of combination of HL1 and Zn2+(A) Fluorescence intensity of different element (1 mmol∙L-1) in presence of ligand HL1 (1 mmol∙L-1) at 500 nm; (B) Plot of fluorescence intensity against different concentration of Zn2+ at 500 nm (a: 1 mmol∙L-1 Zn2+; b: 0.5 mmol∙L-1 Zn2+; c: 0.1 mmol∙L-1 Zn2+; d: 0.05 mmol∙L-1 Zn2+; e: 0.01 mmol∙L-1 Zn2+)
Figure 4 Fluorescence imaging showing the distribution of extracellular Zn2+ in Arabidopsis leaves(A) Mature Arabidopsis leaves pre-treated with different concentrations of ZnSO4 and HL1 (1 mmol∙L-1) for 4 h, a1 and a2 for control, b1 and b2 for 0.3 μmol∙L-1 ZnSO4, c1 and c2 for 3 μmol∙L-1 ZnSO4, d1 and d2 for 30 μmol∙L-1 ZnSO4, e1 and e2 for 300 μmol∙L-1 ZnSO4; (B) The fluorescence intensity of trichome in Arabidopsis leaf with different concentration of ZnSO4 and HL1 (1 mmol∙L-1) for 4 h, a for control, b for 0.3 μmol∙L-1 ZnSO4, c for 3 μmol∙L-1 ZnSO4, d for 30 μmol∙L-1 ZnSO4, and e for 300 μmol∙L-1 ZnSO4. Blue fluorescence represents Zn2+ and red fluorescence for chlorophyll. Bar=100 μm
Figure 6 Fluorescence images showing the distribution of extracellular Zn2+ in Arabidopsis roots(A) Two-centimeter-long Arabidopsis root pre-treated with different concentrations of ZnSO4 and HL1 (1 mmol∙L-1) for 4 h, A1, A2, and A3 for control, 30 μmol∙L-1 ZnSO4 and 300 μmol∙L-1 ZnSO4, respectively (Bar=100 μm); (B) A1, A2, and A3 corresponding to the fluorescence intensity of Arabidopsis root treated with different concentrations of ZnSO4 for 0, 30, and 300 μmol∙L-1. Blue fluorescence represents Zn2+.
Figure 7 Fluorescence images showing the distribution of extracellular Zn2+ in Setaria italic leaves(A) The center of leaf; (B) The margin of leaf; (C) The apex of leaf. Blue fluorescence represents Zn2+ and red fluorescence for chlorophyll. Bar=100 μm
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