Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (4): 444-456.DOI: 10.11983/CBB22088
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Zhang Mingzhu1, Qin Huaguang1, Mu Dan1, Yang Longyu1, Li Hu1, Qi Zehua1, Wang Yuxi1, Zhang Yongcheng1, Ye Lili1, Yin Wenjing2, Wang Shuyuan1, Rao Yuchun2,*(), Wu Yan1,*(
)
Received:
2022-04-26
Revised:
2022-06-28
Online:
2022-07-01
Published:
2022-07-14
Contact:
Rao Yuchun,Wu Yan
About author:
First author contact: These authors contributed equally to this paper
Zhang Mingzhu, Qin Huaguang, Mu Dan, Yang Longyu, Li Hu, Qi Zehua, Wang Yuxi, Zhang Yongcheng, Ye Lili, Yin Wenjing, Wang Shuyuan, Rao Yuchun, Wu Yan. Antioxidant Activity of Tea Polysaccharide and Its Protective Mechanism Against Oxidative Damage[J]. Chinese Bulletin of Botany, 2022, 57(4): 444-456.
Figure 3 Effects of Wan tea polysaccharides (WTPs) on cell physiological state (A) Morphology of mitochondria in control cells; (B) Zoom in micrograph from selected regions from different treated cells and their intensity analysis; (C) Morphology of mitochondria in WTPs incubated cells; (D) Zoom in micrograph from selected regions from different treated cells and their intensity analysis. DIC: Differential interference contrast. Bars=20 μm
Figure 4 Protective effect of Wan tea polysaccharides (WTPs) on demaged cells (A) Effects of different concentrations of exogenous ·OH on the survival rate of HepG-2 cells; (B) Effects of different concentrations of WTPs on HepG-2 cell survival rate induced by ·OH. Compared with control group, *P<0.05, **P<0.01; compared with model group, #P<0.05, ##P<0.01
Figure 5 Effect of Wan tea polysaccharides (WTPs) on the viability of RAW264.7 cells induced by PMA (A) Effects of endogenous ·OH induced by different concentration of PMA on the survival rate of RAW264.7 cells; (B) The effect of different concentration of PMA on morphological changes of RAW264.7 cells (bars=20 μm); (C) Effects of different concentration of WTPs on RAW264.7 cells survival rate induced by endogenous ·OH. Compared with control group, *P<0.05, **P<0.01; compared with model group, #P<0.05, ##P<0.01
Group | GSH-PX | CAT |
---|---|---|
(U∙mL-1) | (U∙mL-1) | |
Control | 0.45±0.08 | 1.08±0.12 |
Model | 0.13±0.04** | 0.59±0.04** |
100 µg∙mL-1 WTPs | 0.16±0.04** | 0.57±0.06** |
200 µg∙mL-1 WTPs | 0.15±0.04** | 0.60±0.07** |
400 µg∙mL-1 WTPs | 0.12±0.03** | 0.62±0.05** |
800 µg∙mL-1 WTPs | 0.13±0.02** | 0.57±0.08** |
Table 1 Effects of Wan tea polysaccharides (WTPs) on the content of glutathione reductase (GSH-PX) and catalase (CAT) in oxidation-damaged RAW264.7 cells (means±SD)
Group | GSH-PX | CAT |
---|---|---|
(U∙mL-1) | (U∙mL-1) | |
Control | 0.45±0.08 | 1.08±0.12 |
Model | 0.13±0.04** | 0.59±0.04** |
100 µg∙mL-1 WTPs | 0.16±0.04** | 0.57±0.06** |
200 µg∙mL-1 WTPs | 0.15±0.04** | 0.60±0.07** |
400 µg∙mL-1 WTPs | 0.12±0.03** | 0.62±0.05** |
800 µg∙mL-1 WTPs | 0.13±0.02** | 0.57±0.08** |
Figure 6 Repair effect of Wan tea polysaccharides (WTPs) on oxidative damage of HepG-2 cells (A) Confocal imaging of ·OH content in cells of different treatment groups (bars=20 μm); (B) Comparison of fluorescence intensity of ·OH in cells of different treatment groups. Compared with control group, *P<0.05, **P<0.01; compared with model group, #P<0.05, ##P<0.01.
Figure 7 Effect of Wan tea polysaccharides (WTPs) on PMA-induced oxidative damage of RAW264.7 macrophages (A) Confocal imaging of ·OH content in cells of different treatment groups (bars=20 μm); (B) Comparison of fluorescence intensity of ·OH in cells of different treatment groups. Compared with control group, *P<0.05, **P<0.01; compared with model group, #P<0.05, ##P<0.01
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