Expression Analysis of Five MiRNAs and Their Precursors During Somatic Embryogenesis in Larch

doi:10.3724/SP.J.1259.2012.00462

Abstract

Abstract: We used the homolog search strategy or RACE to obtain 5 precursor microRNAs (miRNAs) of larch, Larix leptolepis. Mature miRNAs shared high similarity among plant species, and non-mature sequences showed distinct similarity related to genetic relationships. Expression profiles of the 5 precursor and mature miRNAs were examined during 8 developmental stages with quantitative RT-PCR. The expression of precursor and mature miRNAs changed dynamically during developmental stages, with major peaks in expression for mature miRNAs in late cotyledonous embryos, which might be associated with promoting embryo dormancy. However, miR397 and miR408 showed minor peaks in expression at PEMIII, miR398 at the early single embryo stage, and miR156 and miR166 at the early cotyledonous embryo stage, which might be related to regulation to maintain a thin cell wall, proton transfer between photo systems, and formation of shoot meristem. However, the expression of mature miRNAs was not linearly associated with that of precursor miRNAs, which might be regulated at several levels. Our study sheds light on miRNA evolution, expression regulation of mature miRNAs, and regulation of somatic embryogenesis in larch.

Key words: expression analysis, Larix leptolepis, mature miRNA, precursor miRNA, somatic embryogenesis

Junhong Zhang, Shougong Zhang, Tao Wu, Suying Han, Wenhua Yang, Liwang Qi. Expression Analysis of Five MiRNAs and Their Precursors During Somatic Embryogenesis in Larch[J]. Chinese Bulletin of Botany, 2012, 47(5): 462-473.

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URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2012.00462

https://www.chinbullbotany.com/EN/Y2012/V47/I5/462

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