Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (5): 588-595.DOI: 10.11983/CBB20019
• TECHNIQUES AND METHODS • Previous Articles Next Articles
Jianhua Yue1,3, Yan Dong2, Xiaohua Wang1, Peixia Sun1, Siying Wang1, Xinnian Zhang1, Yan Zhang1,3,*()
Received:
2020-02-10
Accepted:
2020-05-08
Online:
2020-09-01
Published:
2020-09-03
Contact:
Yan Zhang
Jianhua Yue, Yan Dong, Xiaohua Wang, Peixia Sun, Siying Wang, Xinnian Zhang, Yan Zhang. A Regeneration System for Organogenesis and Somatic Embryogenesis Using Leaves of Agapanthus praecox as Explants[J]. Chinese Bulletin of Botany, 2020, 55(5): 588-595.
PIC concentration (mg·L-1) | Leaf maturity | Induction rate (%) | Callus size (cm) |
---|---|---|---|
1.5 | 1-2 | 52.38 ab | 0.93 b |
3-4 | 42.86 ab | 0.67 cd | |
5-6 | 38.10 b | 0.63 de | |
2.0 | 1-2 | 85.71 a | 1.07 a |
3-4 | 57.14 ab | 0.77 c | |
5-6 | 38.10 b | 0.53 e |
Table 1 Effects of leaf maturity on callus induction
PIC concentration (mg·L-1) | Leaf maturity | Induction rate (%) | Callus size (cm) |
---|---|---|---|
1.5 | 1-2 | 52.38 ab | 0.93 b |
3-4 | 42.86 ab | 0.67 cd | |
5-6 | 38.10 b | 0.63 de | |
2.0 | 1-2 | 85.71 a | 1.07 a |
3-4 | 57.14 ab | 0.77 c | |
5-6 | 38.10 b | 0.53 e |
PIC concentration (mg·L-1) | Leaf meristematic position (cm) | Induction rate (%) | Callus size (cm) |
---|---|---|---|
1.5 | 0.0-0.5 | 61.90 a | 0.67 ab |
0.5-1.0 | 42.86 ab | 0.57 b | |
1.0-1.5 | 38.09 ab | 0.57 b | |
2.0 | 0.0-0.5 | 66.48 a | 0.73 a |
0.5-1.0 | 47.62 ab | 0.70 ab | |
1.0-1.5 | 19.05 b | 0.60 ab |
Table 2 Effects of different leaf meristematic zones on callus induction
PIC concentration (mg·L-1) | Leaf meristematic position (cm) | Induction rate (%) | Callus size (cm) |
---|---|---|---|
1.5 | 0.0-0.5 | 61.90 a | 0.67 ab |
0.5-1.0 | 42.86 ab | 0.57 b | |
1.0-1.5 | 38.09 ab | 0.57 b | |
2.0 | 0.0-0.5 | 66.48 a | 0.73 a |
0.5-1.0 | 47.62 ab | 0.70 ab | |
1.0-1.5 | 19.05 b | 0.60 ab |
PIC concentration (mg·L-1) | Leaf transection | Induction rate (%) | Callus size (cm) |
---|---|---|---|
1.5 | Middle | 80.95 a | 0.77 a |
Edge | 76.19 a | 0.67 ab | |
2.0 | Middle | 57.14 a | 0.67 ab |
Edge | 42.86 a | 0.57 b |
Table 3 Effects of leaf transection on callus induction
PIC concentration (mg·L-1) | Leaf transection | Induction rate (%) | Callus size (cm) |
---|---|---|---|
1.5 | Middle | 80.95 a | 0.77 a |
Edge | 76.19 a | 0.67 ab | |
2.0 | Middle | 57.14 a | 0.67 ab |
Edge | 42.86 a | 0.57 b |
Type | Concentration (mg·L-1) | Induction rate (%) | Adventitious bud length (cm) |
---|---|---|---|
CK | 0.0 | 12.93 e | 1.69 a |
6-BA | 0.1 | 55.78 bc | 1.42 c |
0.3 | 80.27 a | 1.53 bc | |
0.5 | 73.47 ab | 1.59 ab | |
KT | 0.5 | 53.74 c | 1.49 bc |
1.0 | 44.22 cd | 1.52 bc | |
2.0 | 33.33 d | 1.19 c |
Table 4 Effects of 6-BA and KT on adventitious bud induction
Type | Concentration (mg·L-1) | Induction rate (%) | Adventitious bud length (cm) |
---|---|---|---|
CK | 0.0 | 12.93 e | 1.69 a |
6-BA | 0.1 | 55.78 bc | 1.42 c |
0.3 | 80.27 a | 1.53 bc | |
0.5 | 73.47 ab | 1.59 ab | |
KT | 0.5 | 53.74 c | 1.49 bc |
1.0 | 44.22 cd | 1.52 bc | |
2.0 | 33.33 d | 1.19 c |
Treatment | Concentration (mg·L-1) | Induction number |
---|---|---|
CK | 0.00 | 19.00 bc |
PIC | 1.00 | 1.63 e |
NPA | 1.00 | 11.13 cd |
GA4+7 | 0.05 | 15.00 c |
PBZ | 0.05 | 29.25 ab |
ABA | 1.00 | 40.97 a |
Table 5 Effects of plant growth regulators (PGRs) on somatic embryo induction
Treatment | Concentration (mg·L-1) | Induction number |
---|---|---|
CK | 0.00 | 19.00 bc |
PIC | 1.00 | 1.63 e |
NPA | 1.00 | 11.13 cd |
GA4+7 | 0.05 | 15.00 c |
PBZ | 0.05 | 29.25 ab |
ABA | 1.00 | 40.97 a |
Type | 6-BA concentration (mg·L-1) | Plantlets number | Root number |
---|---|---|---|
Organogenetic plantlets | 1.0 | 11.17 a | 4.67 c |
2.0 | 10.50 ab | 5.67 bc | |
3.0 | 7.08 b | 5.33 bc | |
Somatic plantlets | 1.0 | 14.67 a | 9.33 a |
2.0 | 12.33 a | 7.67 ab | |
3.0 | 11.33 a | 5.33 bc |
Table 6 Effects of 6-BA on plantlets proliferation
Type | 6-BA concentration (mg·L-1) | Plantlets number | Root number |
---|---|---|---|
Organogenetic plantlets | 1.0 | 11.17 a | 4.67 c |
2.0 | 10.50 ab | 5.67 bc | |
3.0 | 7.08 b | 5.33 bc | |
Somatic plantlets | 1.0 | 14.67 a | 9.33 a |
2.0 | 12.33 a | 7.67 ab | |
3.0 | 11.33 a | 5.33 bc |
Cultivation medium | Survival rate (%) | Leaf number | Plant height (cm) |
---|---|---|---|
Commercial substrate | 100.00 | 4.61 | 9.04 |
Peat:sand=1:3 (v/v) | 88.89 | 5.38 | 10.30 |
Peat:vermiculite=1:2 (v/v) | 94.44 | 4.51 | 8.07 |
Perlite:vermiculite=1:1 (v/v) | 100.00 | 5.28 | 8.56 |
Peat:perlite:vermiculite=1:1:1(v/v/v) | 100.00 | 5.56 | 11.77 |
Table 7 Effects of cultivation medium on plantlets transplanting
Cultivation medium | Survival rate (%) | Leaf number | Plant height (cm) |
---|---|---|---|
Commercial substrate | 100.00 | 4.61 | 9.04 |
Peat:sand=1:3 (v/v) | 88.89 | 5.38 | 10.30 |
Peat:vermiculite=1:2 (v/v) | 94.44 | 4.51 | 8.07 |
Perlite:vermiculite=1:1 (v/v) | 100.00 | 5.28 | 8.56 |
Peat:perlite:vermiculite=1:1:1(v/v/v) | 100.00 | 5.56 | 11.77 |
Figure 1 Plant regeneration of Agapanthus praecox via leaf explants (A) Leaf explants; (B) Callus; (C) Adventitious bud induction; (D) Embryogenic callus induction; (E) Adventitious bud turns green when exposure to light; (F) Globular embryos; (G) Plant regeneration via organogenesis; (H) Plant regeneration via club-shaped embryo in somatic embryogenesis; (I) Proliferation of plantlets via organogenesis; (J) The root system of plantlets via organogenesis; (K) Proliferation of plantlets via somatic embryogenesis; (L) The root system of plantlets via somatic embryogenesis; (M) Transplanting and acclimatization of regenerated plantlets; (N) The root system of transplanted plantlets. (A)-(D) Bars= 0.5 cm; (E)-(L) Bars=1 cm; (M) Bar=5 cm; (N) Bar=10 cm
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