Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (5): 760-769.DOI: 10.11983/CBB22141

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment of Agrobacterium tumefaciens-mediated Genetic Transformation System of Marigold (Tagetes erecta)

Yu Xiaomin1, Wang Yaqin1, Liu Yuhan1, Yi Qingping2, Cheng Wenhan2, Zhu Yu1, Duan Feng1, Zhang Lixue1, He Yanhong1,2,*()   

  1. 1. National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
    2. Hubei Engineering Research Center for Specialty Flowers Biological Breeding, Jingchu University of Technology, Jingmen 448000, China
  • Received:2022-07-02 Accepted:2022-12-02 Online:2023-09-01 Published:2023-09-21
  • Contact: *E-mail:

Abstract: In this study, we used the leaflets of marigold (Tagetes erecta) Milestone Yellow as explants to investigate the major factors impacting the transformation efficiency of Agrobacterium-mediated method. The factors included antibiotic concentration, strain type, bacterial concentration, infection time, co-culture time, acetosyringone concentration and anti-browning agent type. We found that the suitable concentrations of cefotaxim sodium salt and kanamycin sulfate were 100 mg·L-1 and 10 mg·L-1, respectively. We also found that the strain EHA105 had the highest transformation efficiency up to 4%. The best infection conditions for EHA105 were at bacterial concentration of 0.1 for OD600, infected for 5 minutes, and co-cultured for 1 day. In addition, the bud germination rate could be improved by both applying 100 μmol·L-1 acetosyringone during the infection process and adding 0.2 g·L-1 polyvinyl pyrrolidone in the screening medium. This study laid a foundation for marigold gene function research and transgenic breeding.

Key words: Agrobacterium tumefaciens, genetic transformation, leaflet, marigold