Effects of High Air and Root Zone Temperature on Photosynthetic Fluorescence Characteristics of Grape Leaves

doi:10.11983/CBB21122

Abstract

Abstract: In order to explore the effects of different air and root zone temperature stress on the fluorescence characteristics of grape (Vitis vinifera) leaves, we treated potted annual Kyoho seedlings under four different temperature conditions: control (C), high air temperature (HA), high root zone temperature (HR), and combined treatment (CT) of HA and HR. Our results showed that the maximum photochemical efficiency (F_v/F_m) under HR and CT treatments was more significantly reduced than under C and HA conditions. The actual quantum efficiency Y(II) of the PSII under HR and CT were significantly reduced, while the quantum yield Y(NPQ) of the non-adjusted energy dissipation, and the redox state of Q_A (1-q_p) were significantly increased compared with the control. At the same time, the variable fluorescence (V_j) of the J point was increased significantly under HR and CT treatments, while the quantum yield (φ_Eo) used for electron transfer and the performance index based on absorption of light energy (PI_ABS) were significantly reduced. In addition, the number of active reaction centers per unit area (RC/CS_m) under HR and CT treatments was decreased significantly, while the relative variable fluorescence (W_k) after normalization was increased significantly. Taken together, our study indicated that high temperature in root zone is a key stress affecting leaf fluorescence characteristics by causing damage on the PSII receptor side. High air temperature aggravated the damage on PSII caused by high root zone temperature.

Key words: grapes, high air temperature, high root zone temperature, chlorophyll fluorescence

Hao Wang, Ming Wang, Ting Liang, Yuxin Yao, Yuanpeng Du, Zhen Gao. Effects of High Air and Root Zone Temperature on Photosynthetic Fluorescence Characteristics of Grape Leaves[J]. Chinese Bulletin of Botany, 2022, 57(2): 209-216.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB21122

https://www.chinbullbotany.com/EN/Y2022/V57/I2/209

Figures/Tables 4

Figure 1 The influence of different air temperature/root zone temperature treatments on the Fv/Fm (A), Y(II) (B), Y(NPQ) (C) and Y(NO) (D) of Kyoho grape leaves Different lowercase letters indicate significant differences (P<0.05). Fv/Fm: PSII maximum photochemical efficiency; Y(II): PSII actual photochemical quantum yield; Y(NPQ): Regulated energy dissipation quantum yield; Y(NO): Non-regulated energy dissipation quantum yield; PAR: Photosynthetically active radiation; CK: Control; T1: High air temperature; T2: High root zone temperature; T3: Cross processing of high air temperature and high root zone temperature

Figure 2 The influence of different air temperature/root zone temperature treatments on the Y(CEF) (A) and 1-qP (B) of Kyoho grape leaves Different lowercase letters indicate significant differences (P<0.05). Y(CEF): Circular electron transfer effective quantum yield; 1-qP: QA redox state; PAR, CK and T1-T3 are shown in Figure 1.

Figure 3 Effect of different air temperature/root zone temperature treatments on the actual chlorophyll fluorescence induction curve (A) and the relative variable chlorophyll fluorescence induction curve (B) CK and T1-T3 are the same as shown in Figure 1.

Figure 4 Different lowercase letters indicate significant differences (P<0.05). RC/CSm: The number of active reaction centers per unit area; φEo: Quantum yield used for electron transfer; Wk: Relatively variable fluorescence of K point; Vj: Relatively variable fluorescence of J point; PIABS: The performance index; φDo: Quantum ratio used for heat dissipation; CK and T1-T3 are shown in Figure 1.

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