Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (2): 197-208.DOI: 10.11983/CBB21074

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Effects of Overexpression of MtVP1 on Potato Phenotypes and Sugar Metabolism

Jianwu Wang1, Wenjuan Wang2, Weiwei Xiang1, Huiping Dai3, Haiqing Wang2, Xiangxiang Qu1, Furen Kang1,*()   

  1. 1Shaanxi Key Laboratory of Ecological Restoration in Shanbei Mining Area, Yulin University, Yulin 719000, China
    2Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
    3College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China
  • Received:2021-05-02 Accepted:2021-10-13 Online:2022-03-01 Published:2022-03-24
  • Contact: Furen Kang

Abstract: Type I H+-PPase is involved in gluconeogenesis and sucrose catabolism. Different types of mutants of Arabidopsis thaliana type I H+-PPase gene fed with different sugars (sucrose, glucose and fructose) showed various phenotypes. Therefore, it is speculated that type I H+-PPase may contain other mechanisms that affect sugar metabolism. In order to further clarify the effect of the enzyme on sugar metabolism, phenotypes of the MtVP1 overexpressed potato Weishu 4 were observed under different culture conditions. The fluctuation of sugar content was monitored, and the transcription profile was analyzed. The results showed that the MtVP1 overexpressed potato grew red stem, purple flower, and more developed trichomes. Furthermore, tubers per plant decreased in number, but became larger in size, and shriveled up faster. However, the transgenic plant had significantly decreased starch, glucose, and fructose contents in tubers, and glucose and fructose contents in buds. At the same time, feeding fructose significantly reduced the anthocyanin, and up-regulated the gene expression of fructose-1,6-diphosphate and fructose-2,6-diphosphate by 3-7 fold in the transgenic potato. The results could shed light on further studies of the physiological function of type I H+-PPase from the perspective of sugar metabolism.

Key words: type I membrane-associated H+-PPase, fructose-1,6-bisphosphatase, fructose-2,6-bisphosphatase, sugar metabolism, fructose metabolism