Protocols for Small RNA FISH in Plants

doi:10.11983/CBB21057

Abstract

Abstract: Small RNAs are a type of small nucleotide molecules that are essential for plant growth and development, playing a key role in variety of life processes and in response to stresses. Research on the location of small RNAs could discover their functions in plants. Small RNA FISH is a qualitative or semi-quantitative analysis of small RNA in organisms by fluorescence detection technology. At present, this technology has been widely used in animals, but it is still less applied in plants. This article introduces the specific operation procedures and attentions based on ultra-high resolution microscopy that combines locked nucleic acid (LNA) probe in situ hybridization with immunofluorescence. This protocol can be used to detect the expression and localization of small RNA in plant tissues.

Key words: fluorescent in situ hybridization, small RNA, plants

Binbin Hu, Zhihui Xue, Cui Zhang. Protocols for Small RNA FISH in Plants[J]. Chinese Bulletin of Botany, 2021, 56(3): 330-338.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB21057

https://www.chinbullbotany.com/EN/Y2021/V56/I3/330

Figures/Tables 2

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