Chinese Bulletin of Botany ›› 2021, Vol. 56 ›› Issue (3): 330-338.DOI: 10.11983/CBB21057

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Protocols for Small RNA FISH in Plants

Binbin Hu1,2, Zhihui Xue1, Cui Zhang1,2,*()   

  1. 1Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2020-04-02 Accepted:2021-05-07 Online:2021-05-01 Published:2021-05-07
  • Contact: Cui Zhang

Abstract: Small RNAs are a type of small nucleotide molecules that are essential for plant growth and development, playing a key role in variety of life processes and in response to stresses. Research on the location of small RNAs could discover their functions in plants. Small RNA FISH is a qualitative or semi-quantitative analysis of small RNA in organisms by fluorescence detection technology. At present, this technology has been widely used in animals, but it is still less applied in plants. This article introduces the specific operation procedures and attentions based on ultra-high resolution microscopy that combines locked nucleic acid (LNA) probe in situ hybridization with immunofluorescence. This protocol can be used to detect the expression and localization of small RNA in plant tissues.

Key words: fluorescent in situ hybridization, small RNA, plants