Genetic Relationship of Physalis Plants Revealed by Simple Sequence Repeat Markers

doi:10.11983/CBB18027

Abstract

Abstract: In recent years, Physalis plants have attracted increasing attention worldwide due to their high nutritional value, edible fruit, and potential medicinal value. In this study, simple sequence repeat (SSR) markers were used to assess genetic relationships with 22 samples of four Physalis species mainly distributed in China. Twenty SSR primer pairs produced 118 loci, 90.7% (107) of which showed polymorphism. The average interspecies similarity coefficient was 0.501, which indicates a degree of genetic relationship among Physalis species. The results of UPGMA dendrography and PCoA plotting were similar, and all Physalis samples were grouped into two clusters. All P. alkekengi var. francheti samples, distant from any other Physalis species, constituted a separate cluster, which confirmed findings of previous studies. This study also indicated that SSR markers are rich in genetic information and could be used to assess the genetic diversity of Physalis species which provides rich useful information for protecting the Physalis germplasm resource and an important foundation for molecular assisted-breeding programs with Physalis.

Key words: Physalis, genetic relationship, SSR markers

Zhu Yujia, Jiao Kaili, Luo Xiujun, Feng Shangguo, Wang Huizhong. Genetic Relationship of Physalis Plants Revealed by Simple Sequence Repeat Markers[J]. Chinese Bulletin of Botany, 2018, 53(3): 305-312.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB18027

https://www.chinbullbotany.com/EN/Y2018/V53/I3/305

Figures/Tables 6

References 34

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No.	Species name	Code	Origin
1	Physalis minima	XSJ1	Qianxi, Tangshan, Hebei
2	P. minima	XSJ2	Mudan, Heze, Shandong
3	P. minima	XSJ3	Lou’an, Anhui
4	P. minima	XSJ4	Lishui, Zhejiang
5	P. angulata	KZ1	Jianggan, Hangzhou, Zhejiang
6	P. angulata	KZ2	Xiaoshan, Hangzhou, Zhejiang
7	P. angulata	KZ3	Luotian, Huanggang, Hubei
8	P. angulata	KZ4	Honghe, Yunnan
9	P. angulata	KZ5	Najing, Jiangsu
10	P. angulata	KZ6	Linhai, Taizhou, Zhejiang
11	P. angulata	KZ7	Wenzhou, Zhejiang
12	P. angulata	KZ8	Pujiang, Jinhua, Zhejiang
13	P. angulata	KZ9	Xiajin, Dezhou, Shandong
14	P. alkekengi var. francheti	SJ1	Faku, Shenyang, Liaoning
15	P. alkekengi var. francheti	SJ2	Donggang, Dandong, Liaoning
16	P. alkekengi var. francheti	SJ3	Nong’an, Changchun, Jilin
17	P. alkekengi var. francheti	SJ4	Zoucheng, Jinan, Shandong
18	P. pubescens	MSJ1	Faku, Shenyang, Liaoning
19	P. pubescens	MSJ2	Nong’an, Changchun, Jilin
20	P. pubescens	MSJ3	Chaoyang, Zhaodong, Heilongjiang
21	P. pubescens	MSJ4	Mudanjiang, Heilongjiang
22	P. pubescens	MSJ5	Hulunbeir, Inner Mongolia

No.	Species name	Code	Origin
1	Physalis minima	XSJ1	Qianxi, Tangshan, Hebei
2	P. minima	XSJ2	Mudan, Heze, Shandong
3	P. minima	XSJ3	Lou’an, Anhui
4	P. minima	XSJ4	Lishui, Zhejiang
5	P. angulata	KZ1	Jianggan, Hangzhou, Zhejiang
6	P. angulata	KZ2	Xiaoshan, Hangzhou, Zhejiang
7	P. angulata	KZ3	Luotian, Huanggang, Hubei
8	P. angulata	KZ4	Honghe, Yunnan
9	P. angulata	KZ5	Najing, Jiangsu
10	P. angulata	KZ6	Linhai, Taizhou, Zhejiang
11	P. angulata	KZ7	Wenzhou, Zhejiang
12	P. angulata	KZ8	Pujiang, Jinhua, Zhejiang
13	P. angulata	KZ9	Xiajin, Dezhou, Shandong
14	P. alkekengi var. francheti	SJ1	Faku, Shenyang, Liaoning
15	P. alkekengi var. francheti	SJ2	Donggang, Dandong, Liaoning
16	P. alkekengi var. francheti	SJ3	Nong’an, Changchun, Jilin
17	P. alkekengi var. francheti	SJ4	Zoucheng, Jinan, Shandong
18	P. pubescens	MSJ1	Faku, Shenyang, Liaoning
19	P. pubescens	MSJ2	Nong’an, Changchun, Jilin
20	P. pubescens	MSJ3	Chaoyang, Zhaodong, Heilongjiang
21	P. pubescens	MSJ4	Mudanjiang, Heilongjiang
22	P. pubescens	MSJ5	Hulunbeir, Inner Mongolia

Primer name	Primer sequence (5'-3')	Repeat type	T_m	No. of loci	Polymorphic loci	Polymorphism rate (%)
SSR2	F: CATTGGGTTTCGCATCCAT	AG	60	6	6	100.0
	R: AGACAAGCCTAGGGGAAAGG
SSR9	F: TGCTCCGAGTTTTAGGGTTC	AG	60	8	7	87.5
	R: GCAGTTGGTAAAGTTGAGAGACG
SSR10	F: GCTTCCTATTGTGTTGCCTGA	AT	58	5	4	80.0
	R: ACTTTGGGTTTCGGGAATTG
SSR11	F: CAGCTGAAATAAGAGAGTGATTGG	AG	57	4	3	75.0
	R: CCCTCTTTTTCTCCTCCGAGT
SSR13	F: GCGGAATCCATTGTTTTTCA	AC	58	9	8	88.9
	R: CCGATGGAGTATAGTCACGCAAA
SSR15	F: GCTTGTTGATCAGCTTTCTTTG	AT	57	7	6	85.7
	R: TGGATCATAACCTTGCTAATGC
SSR36	F: ATGAACCACATGTCGGAGGA	AG	58	7	6	85.7
	R: GGGGATCCAAACGAAGTGTA
SSR54	F: CGGCTGGTATGCTTACAAAGAT	AC	58	4	4	100.0
	R: GCACTTCCACTGTTTTTAACTTCC
SSR55	F: CACCTACATAGGCAGCCAAAA	AG	58	7	6	85.7
	R: ATTTGTGGGCGGAGGAAG
SSR57	F: AGTGAAAAGCAGCCCATTCT	AT	56	9	8	88.9
	R: GGCGAAGCTGAATTGAAAAA
SSR67	F: GCTTCTGTTCCATTATTCACCA	AG	56	5	5	100.0
	R: GCAGTGTGGGATCAATCAAT
SSR68	F: GAAGCAAACAACTACACCCAAA	AG	56	8	8	100.0
	R: AAGCCTCGGATTTCATAGCA
SSR77	F: CATACCATAACTCCCCATCTCTC	AG	57	4	3	75.0
	R: TGCCGATTCTGATTTCTTCC
SSR92	F: TGGTTTGAGGATCAAGAAAGAA	AAG	56	5	4	80.0
	R: GTGGTATCAACGCAGAGTGG
SSR107	F: CATCCAACACCAGAAATACGC	AAG	58	4	4	100.0
	R: TCCAACTTTATCATTTCTTCCAC
SSR110	F: CACCCATATCCCAATCTTCTTC	CTT	60	4	4	100.0
	R: GGGTAATTTTCACGGGGAAT
SSR112	F: CTACGCCTACCACTTGCACA	TCT	60	11	11	100.0
	R: CAGTGGAAGCCTCAAGATCC
SSR118	F: AATCAAGGGTCAGAAGAAATGG	ATC	58	2	2	100.0
	R: GCAAGAATGGATGTGGGTGT
SSR123	F: TCAGTGGAGCGCGTATATCT	ATC	60	5	5	100.0
	R: GCGATCTCACCAAACCTCTC
SSR127	F: TTGGTTTGGCATAACTGCAA	AAT	58	4	3	75.0
	R: GGTTTGCAACTCTCATGCTG
Average	-	-	-	5.9	5.4	90.4
Total	-	-	-	118	107	-

Primer name	Primer sequence (5'-3')	Repeat type	T_m	No. of loci	Polymorphic loci	Polymorphism rate (%)
SSR2	F: CATTGGGTTTCGCATCCAT	AG	60	6	6	100.0
	R: AGACAAGCCTAGGGGAAAGG
SSR9	F: TGCTCCGAGTTTTAGGGTTC	AG	60	8	7	87.5
	R: GCAGTTGGTAAAGTTGAGAGACG
SSR10	F: GCTTCCTATTGTGTTGCCTGA	AT	58	5	4	80.0
	R: ACTTTGGGTTTCGGGAATTG
SSR11	F: CAGCTGAAATAAGAGAGTGATTGG	AG	57	4	3	75.0
	R: CCCTCTTTTTCTCCTCCGAGT
SSR13	F: GCGGAATCCATTGTTTTTCA	AC	58	9	8	88.9
	R: CCGATGGAGTATAGTCACGCAAA
SSR15	F: GCTTGTTGATCAGCTTTCTTTG	AT	57	7	6	85.7
	R: TGGATCATAACCTTGCTAATGC
SSR36	F: ATGAACCACATGTCGGAGGA	AG	58	7	6	85.7
	R: GGGGATCCAAACGAAGTGTA
SSR54	F: CGGCTGGTATGCTTACAAAGAT	AC	58	4	4	100.0
	R: GCACTTCCACTGTTTTTAACTTCC
SSR55	F: CACCTACATAGGCAGCCAAAA	AG	58	7	6	85.7
	R: ATTTGTGGGCGGAGGAAG
SSR57	F: AGTGAAAAGCAGCCCATTCT	AT	56	9	8	88.9
	R: GGCGAAGCTGAATTGAAAAA
SSR67	F: GCTTCTGTTCCATTATTCACCA	AG	56	5	5	100.0
	R: GCAGTGTGGGATCAATCAAT
SSR68	F: GAAGCAAACAACTACACCCAAA	AG	56	8	8	100.0
	R: AAGCCTCGGATTTCATAGCA
SSR77	F: CATACCATAACTCCCCATCTCTC	AG	57	4	3	75.0
	R: TGCCGATTCTGATTTCTTCC
SSR92	F: TGGTTTGAGGATCAAGAAAGAA	AAG	56	5	4	80.0
	R: GTGGTATCAACGCAGAGTGG
SSR107	F: CATCCAACACCAGAAATACGC	AAG	58	4	4	100.0
	R: TCCAACTTTATCATTTCTTCCAC
SSR110	F: CACCCATATCCCAATCTTCTTC	CTT	60	4	4	100.0
	R: GGGTAATTTTCACGGGGAAT
SSR112	F: CTACGCCTACCACTTGCACA	TCT	60	11	11	100.0
	R: CAGTGGAAGCCTCAAGATCC
SSR118	F: AATCAAGGGTCAGAAGAAATGG	ATC	58	2	2	100.0
	R: GCAAGAATGGATGTGGGTGT
SSR123	F: TCAGTGGAGCGCGTATATCT	ATC	60	5	5	100.0
	R: GCGATCTCACCAAACCTCTC
SSR127	F: TTGGTTTGGCATAACTGCAA	AAT	58	4	3	75.0
	R: GGTTTGCAACTCTCATGCTG
Average	-	-	-	5.9	5.4	90.4
Total	-	-	-	118	107	-

Measurement	Similarity coefficient
Average interspecies genetic similarity coefficient	0.501±0.074
P. angulata vs P. minima	0.600±0.042
P. alkekengi var. francheti vs P. minima	0.437±0.036
P. pubescens vs P. minima	0.570±0.037
P. angulata vs P. alkekengi var. francheti	0.444±0.044
P. angulata vs P. pubescens	0.514±0.043
P. pubescens vs P. alkekengi var. francheti	0.382±0.040