Chinese Bulletin of Botany ›› 2018, Vol. 53 ›› Issue (3): 313-321.DOI: 10.11983/CBB17193
Special Issue: 药用植物专辑 (2018年53卷3期)
• EXPERIMENTAL COMMUNICATIONS • Previous Articles Next Articles
Ren Mengyun1,2, Du Leshan1, Chen Yanjun1,2, Zhang Dun2, Shen Qi2, Guan Xiao1,*(), Zhang Yindong2,*()
Received:
2017-10-18
Accepted:
2018-04-30
Online:
2018-05-01
Published:
2018-09-11
Contact:
Guan Xiao,Zhang Yindong
Ren Mengyun, Du Leshan, Chen Yanjun, Zhang Dun, Shen Qi, Guan Xiao, Zhang Yindong. Analysis on Genetic Diversity of Cynomorium songaricum by ITS Sequence[J]. Chinese Bulletin of Botany, 2018, 53(3): 313-321.
Sample No. | Location | Longitude E (°) | Latitude N (°) | Height (m) | Number | |
---|---|---|---|---|---|---|
R1 | Zhangye, Gansu | 102.522 | 36.822 | 1803 | 11 | |
R2 | Minqin, Gansu | 100.772 | 39.223 | 1370 | 17 | |
R3 | Jinchang, Gansu | 102.54 | 38.44 | 1466 | 7 | |
R4 | Sandan, Gansu | 101.118 | 38.897 | 2020 | 10 | |
R5 | Gaotai, Gansu | 99.086 | 39.787 | 1339 | 11 | |
R6 | Sunan, Gansu | 99.176 | 39.604 | 1395 | 10 | |
R7 | Sunan, Gansu | 99.474 | 39.445 | 1391 | 12 | |
R8 | Jiuquan, Gansu | 98.55 | 40.3 | 1232 | 4 | |
R9 | Yumen, Gansu | 97.196 | 40.516 | 1395 | 10 | |
R10 | Guazhou, Gansu | 95.585 | 40.212 | 1343 | 11 | |
R11 | Dunhuang, Gansu | 94.583 | 40.368 | 1033 | 11 | |
R12 | Subei, Gansu | 96.619 | 39.397 | 2355 | 10 | |
R13 | Delingha, Qinghai | 97.334 | 37.206 | 2780 | 11 | |
R14 | Geermu, Qinghai | 92.839 | 36.701 | 2790 | 7 | |
R15 | Dulan, Qinghai | 98.164 | 36.476 | 2972 | 12 | |
R16 | Wulan, Qinghai | 98.622 | 36.458 | 2917 | 12 | |
R17 | Gonghe, Qinghai | 100.183 | 36.446 | 2904 | 11 | |
R18 | Guide, Qinghai | 101.639 | 36.219 | 2389 | 11 |
Table 1 Location of 18 populations of Cynomorium songaricum
Sample No. | Location | Longitude E (°) | Latitude N (°) | Height (m) | Number | |
---|---|---|---|---|---|---|
R1 | Zhangye, Gansu | 102.522 | 36.822 | 1803 | 11 | |
R2 | Minqin, Gansu | 100.772 | 39.223 | 1370 | 17 | |
R3 | Jinchang, Gansu | 102.54 | 38.44 | 1466 | 7 | |
R4 | Sandan, Gansu | 101.118 | 38.897 | 2020 | 10 | |
R5 | Gaotai, Gansu | 99.086 | 39.787 | 1339 | 11 | |
R6 | Sunan, Gansu | 99.176 | 39.604 | 1395 | 10 | |
R7 | Sunan, Gansu | 99.474 | 39.445 | 1391 | 12 | |
R8 | Jiuquan, Gansu | 98.55 | 40.3 | 1232 | 4 | |
R9 | Yumen, Gansu | 97.196 | 40.516 | 1395 | 10 | |
R10 | Guazhou, Gansu | 95.585 | 40.212 | 1343 | 11 | |
R11 | Dunhuang, Gansu | 94.583 | 40.368 | 1033 | 11 | |
R12 | Subei, Gansu | 96.619 | 39.397 | 2355 | 10 | |
R13 | Delingha, Qinghai | 97.334 | 37.206 | 2780 | 11 | |
R14 | Geermu, Qinghai | 92.839 | 36.701 | 2790 | 7 | |
R15 | Dulan, Qinghai | 98.164 | 36.476 | 2972 | 12 | |
R16 | Wulan, Qinghai | 98.622 | 36.458 | 2917 | 12 | |
R17 | Gonghe, Qinghai | 100.183 | 36.446 | 2904 | 11 | |
R18 | Guide, Qinghai | 101.639 | 36.219 | 2389 | 11 |
Haplotype | Variable sites | Abundance | ||||||
---|---|---|---|---|---|---|---|---|
63 | 86 | 197 | 274 | 421 | 605 | 623 | ||
H1 | G | G | G | T | G | C | G | 143 |
H2 | A | G | G | T | G | C | T | 3 |
H3 | G | A | G | T | G | C | G | 1 |
H4 | G | A | G | T | G | G | G | 2 |
H5 | A | G | G | T | G | C | G | 26 |
H6 | G | G | A | T | G | C | G | 5 |
H7 | G | G | A | G | G | C | G | 1 |
H8 | G | G | G | T | G | G | G | 4 |
H9 | G | G | G | T | A | C | G | 3 |
Table 2 Variable sites of ITS sequence haplotypes of Cynomorium songaricum
Haplotype | Variable sites | Abundance | ||||||
---|---|---|---|---|---|---|---|---|
63 | 86 | 197 | 274 | 421 | 605 | 623 | ||
H1 | G | G | G | T | G | C | G | 143 |
H2 | A | G | G | T | G | C | T | 3 |
H3 | G | A | G | T | G | C | G | 1 |
H4 | G | A | G | T | G | G | G | 2 |
H5 | A | G | G | T | G | C | G | 26 |
H6 | G | G | A | T | G | C | G | 5 |
H7 | G | G | A | G | G | C | G | 1 |
H8 | G | G | G | T | G | G | G | 4 |
H9 | G | G | G | T | A | C | G | 3 |
Population code | Samples | Haptotypes | Hd |
---|---|---|---|
R1 | 11 | H1, H2, H3, H4 | 0.25974 |
R2 | 17 | H1, H5, H6 | 0.11586 |
R3 | 7 | H1, H5 | 0.43956 |
R4 | 10 | H1, H2, H5 | 0.42632 |
R5 | 11 | H1, H6 | 0.24675 |
R6 | 10 | H1, H7, H8 | 0.19474 |
R7 | 12 | H1, H5 | 0.08333 |
R8 | 4 | H1, H5, H9 | 0.60714 |
R9 | 10 | H1, H8 | 0.10000 |
R10 | 11 | H1, H5, H8 | 0.17749 |
R11 | 11 | H1, H4, H6 | 0.17749 |
R12 | 10 | H1 | 0 |
R13 | 11 | H1 | 0 |
R14 | 7 | H1, H6 | 0.14286 |
R15 | 12 | H1, H9 | 0.08333 |
R16 | 12 | H1, H9 | 0.08333 |
R17 | 11 | H1, H8 | 0.09091 |
R18 | 11 | H1 | 0 |
Total | 188 | 0.29420 |
Table 3 Haplotype diversity and composition of Cynomorium songaricum from18 populations
Population code | Samples | Haptotypes | Hd |
---|---|---|---|
R1 | 11 | H1, H2, H3, H4 | 0.25974 |
R2 | 17 | H1, H5, H6 | 0.11586 |
R3 | 7 | H1, H5 | 0.43956 |
R4 | 10 | H1, H2, H5 | 0.42632 |
R5 | 11 | H1, H6 | 0.24675 |
R6 | 10 | H1, H7, H8 | 0.19474 |
R7 | 12 | H1, H5 | 0.08333 |
R8 | 4 | H1, H5, H9 | 0.60714 |
R9 | 10 | H1, H8 | 0.10000 |
R10 | 11 | H1, H5, H8 | 0.17749 |
R11 | 11 | H1, H4, H6 | 0.17749 |
R12 | 10 | H1 | 0 |
R13 | 11 | H1 | 0 |
R14 | 7 | H1, H6 | 0.14286 |
R15 | 12 | H1, H9 | 0.08333 |
R16 | 12 | H1, H9 | 0.08333 |
R17 | 11 | H1, H8 | 0.09091 |
R18 | 11 | H1 | 0 |
Total | 188 | 0.29420 |
Figure 1 Strict consensus tree based on the ITS sequence of Cynomorium songaricumLength=784, CI=0.949 0, RI=0.911 7, RCI=0.865 2. The num- bers on the branch represent the support rate of MP/ML/BI, respectively.
Figure 2 Haplotype network based on ITS sequence of Cynomorium songaricum from 18 populationsThe size of circles are proportional to the relative frequency of the haplotype; Different colors represent different populations of Cynomorium songaricum.
Sequence | HS (SE) | HT (SE) | GST (SE) | NST (SE) |
---|---|---|---|---|
ITS | 0.179 (0.0390) | 0.277 (0.0820) | 0.353 (0.1914) | 0.408 (0.2038) |
Table 4 Genetic structural parameters of Cynomorium songaricum
Sequence | HS (SE) | HT (SE) | GST (SE) | NST (SE) |
---|---|---|---|---|
ITS | 0.179 (0.0390) | 0.277 (0.0820) | 0.353 (0.1914) | 0.408 (0.2038) |
Source of variation | df | Sum of squares | Variance components | Percentage | FST | P |
---|---|---|---|---|---|---|
Among populations | 17 | 28.833 | 0.07689 Va | 44.57 | 0.44566 | <0.001* |
Within populations | 358 | 34.239 | 0.09564 Vb | 55.43 | ||
Total | 375 | 63.072 | 0.17253 |
Table 5 Analysis of molecular variance for ribosome haplotypes of Cynomorium songaricum
Source of variation | df | Sum of squares | Variance components | Percentage | FST | P |
---|---|---|---|---|---|---|
Among populations | 17 | 28.833 | 0.07689 Va | 44.57 | 0.44566 | <0.001* |
Within populations | 358 | 34.239 | 0.09564 Vb | 55.43 | ||
Total | 375 | 63.072 | 0.17253 |
Figure 3 Mismatch distribution analysis for the populations of Cynomorium songaricum based on ITS sequenceCylindricality represents the expected distribution of variation sites under the population expansion model; dotted line represents the actual distribution of variation sites.
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