The Genetic Diversity of Common Millet (Panicum miliaceum) Germplasm Resources Based on the EST-SSR Markers

doi:10.11983/CBB19037

Abstract

Abstract: The EST-SSR molecular markers of common millet (Panicum miliaceum) were developed by high-throughput sequencing. Using these markers, we assessed the genetic diversity in a panel of 144 common millet accessions collected from different ecotopic regions in China and abroad. It was shown that 80 pairs of these markers were polymorphic, with the efficiency of approximately 40%. The resolution power (Rp) was 0.67-4.67 (mean 2.00) and the amplified product sizes ranged from 50 to 500 bp. Among the examined 144 accessions, 206 allelic variations were identified in 80 loci, with 2-3 alleles at each locus. The Shannon’s diversity index (I) ranged from 0.659 3 (RYW108) to 1.087 2 (RYW124) with an average of 0.859 9. The range of polymorphism information content (PIC) was 0.222 9 (RYW98) -0.717 2 (RYW124) with an average of 0.457 3. Based on UPGMA, these 144 accessions were classified into 3 groups, two of which belonged to the the Northern China spring-sowing ecotopes and one group was mainly from the Loess Plateau spring-summer-sowing ecotopes. Based on Structure (K=4), all the accessions were divided into four groups, of which two groups represented the gene pool originated from the Northern China, whereas the other two groups from the Loess Plateau and abroad accessions. Based on principal component analysis (PCA), the accessions were clustered into seven groups, consistent with their geographic origins.

Key words: Panicum miliaceum, genetic diversity, PCA, EST-SSR markers

Jieli He,Tiantian Shi,Ling Chen,Haigang Wang,Zhijun Gao,Meihong Yang,Ruiyun Wang,Zhijun Qiao. The Genetic Diversity of Common Millet (Panicum miliaceum) Germplasm Resources Based on the EST-SSR Markers[J]. Chinese Bulletin of Botany, 2019, 54(6): 723-732.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB19037

https://www.chinbullbotany.com/EN/Y2019/V54/I6/723

Figures/Tables 11

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Ecotope/abroad	Origin	Number of accession	Total
Northwest spring and summer-sowing ecotope (NWSS)	Xinjiang	4	4
Northern spring-sowing ecotope (NSP)	Qinghai	13	48
	Gansu	11
	Inner Mongolia	14
	Shanxi	10
Loess Plateau spring and summer-sowing ecotope (LPSS)	Shanxi	18	37
	Shaanxi	8
	Ningxia	11
Northeast spring-sowing ecotope (NES)	Heilongjiang	5	9
	Jilin	3
	Liaoning	1
Northern summer-sowing ecotope (NSU)	Hebei	9	13
	Shandong	2
	Anhui	1
	Henan	1
Southern autumn and winter-sowing ecotope (SAW)	Hainan	2	2
Abroad	Former Soviet Union	2	31
	Poland	2
	India	27
Total		144

Ecotope/abroad	Origin	Number of accession	Total
Northwest spring and summer-sowing ecotope (NWSS)	Xinjiang	4	4
Northern spring-sowing ecotope (NSP)	Qinghai	13	48
	Gansu	11
	Inner Mongolia	14
	Shanxi	10
Loess Plateau spring and summer-sowing ecotope (LPSS)	Shanxi	18	37
	Shaanxi	8
	Ningxia	11
Northeast spring-sowing ecotope (NES)	Heilongjiang	5	9
	Jilin	3
	Liaoning	1
Northern summer-sowing ecotope (NSU)	Hebei	9	13
	Shandong	2
	Anhui	1
	Henan	1
Southern autumn and winter-sowing ecotope (SAW)	Hainan	2	2
Abroad	Former Soviet Union	2	31
	Poland	2
	India	27
Total		144

Number	Unicode	Accession name	Origin
1	00000177	Hongmizi	Ningan, Heilongjiang
2	00000750	Baimizi	Shawan, Xinjiang
3	00006653	Jinshu	Hainan
4	00007238	Dahongmizi	Bameng, Inner Mongolia
5	00007478	Baigedami	Huangzhong, Qinghai
6	No unicode	Hongshuzi	Anyang, Henan

Number	Unicode	Accession name	Origin
1	00000177	Hongmizi	Ningan, Heilongjiang
2	00000750	Baimizi	Shawan, Xinjiang
3	00006653	Jinshu	Hainan
4	00007238	Dahongmizi	Bameng, Inner Mongolia
5	00007478	Baigedami	Huangzhong, Qinghai
6	No unicode	Hongshuzi	Anyang, Henan

Ecotope/ abroad	Accessions	Na	Ne	I	Ho	He	PIC
NWSS	4	2.3375±0.5017	2.1517±0.4194	0.7644±0.2178	0.8042±0.2688	0.5944±0.1193	0.3551
NSP	48	2.5750±0.4975	2.3106±0.3211	0.8604±0.1576	0.8228±0.1308	0.5655±0.0604	0.4536
LPSS	37	2.5750±0.4975	2.2803±0.3110	0.8506±0.1534	0.8384±0.1166	0.5615±0.0595	0.4203
NES	9	2.5125±0.5030	2.2435±0.3929	0.8289±0.1823	0.7937±0.1732	0.5737±0.0909	0.4212
NSU	13	2.5625±0.4992	2.2815±0.3527	0.8496±0.1632	0.7946±0.1608	0.5712±0.0667	0.4304
SAW	2	2.2375±0.5092	2.0608±0.4387	0.7347±0.2316	0.7812±0.3265	0.6813±0.2006	0.2156
Domestic	113	2.5750 ±0.4975	2.3122±0.3086	0.8628±0.1554	0.8200±0.1188	0.5625±0.0584	0.4651
Abroad	31	2.5750±0.4975	2.2464±0.2909	0.8387±0.1449	0.8540±0.1193	0.5571±0.0561	0.3896