Characterization of Programmed Cell Death During the Senescence of Root Hairs in Arabidopsis

doi:10.11983/CBB15081

Chinese Bulletin of Botany ›› 2016, Vol. 51 ›› Issue (2): 194-201.DOI: 10.11983/CBB15081

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Characterization of Programmed Cell Death During the Senescence of Root Hairs in Arabidopsis

Lin Li, Kang Tan, Xiuguang Tang, Xiaoting Chao, Chenxi Wen, Zhuangdong Bai Hualing Feng, Wenzhe Liu^*, Hui Su^*

Key Laboratory of Resource Biology and Biotechnology in Western China of Ministry of Education College of Life Science, Northwest University, Xi’an 710069, China

Received:2015-05-18 Revised:2015-06-28 Online:2016-03-01 Published:2016-03-31
Contact: Hui Su

Abstract

Abstract: The root hair is an essential organ for the uptake of nutrients in plants; root hair cells can only survive for 2 to 3 weeks and then die off, but the mechanisms underlying root hair death is not clear. In this study, we investigated the cellular characteristics of root hair death. Arabidopsis dying root hairs underwent protoplast retraction from the cell wall, and some unknown aggregates were observed in the cell. On detecting DNA fragmentation by terminal deoxynucleotide transferase- mediated dUTP nick-end labeling (TUNEL), we found TUNEL-positive nuclei in root hairs. Protoplast retraction and nuclear DNA fragmentation are both considered hallmark features of apoptotic-like programmed cell death in plants. Therefore, we propose that the death of Arabidopsis root hairs belongs to programmed cell death, which is mediated by an intracellular program. As well, fluorescence staining with DAPI demonstrated that in dying root hairs, the nucleus would migrate to a position close to the basement of the root hair. Moreover, the tubular-shaped root hairs finally twist.

Lin Li, Kang Tan, Xiuguang Tang, Xiaoting Chao, Chenxi Wen, Zhuangdong Bai Hualing Feng, Wenzhe Liu, Hui Su. Characterization of Programmed Cell Death During the Senescence of Root Hairs in Arabidopsis[J]. Chinese Bulletin of Botany, 2016, 51(2): 194-201.

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Characterization of Programmed Cell Death During the Senescence of Root Hairs in Arabidopsis

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