植物学报 ›› 2014, Vol. 49 ›› Issue (1): 98-104.DOI: 10.3724/SP.J.1259.2014.00098

• 研究报告 • 上一篇    下一篇

NaHS对NaHCO3胁迫下黑籽南瓜种子萌发及生理特性的影响

孙涌栋*, 罗未蓉, 李新峥, 王广印   

  1. 河南科技学院园艺园林学院, 新乡 453003
  • 收稿日期:2013-04-01 修回日期:2013-07-22 出版日期:2014-01-01 发布日期:2014-01-01
  • 通讯作者: 孙涌栋
  • 基金资助:

    河南省青年骨干教师资助计划项目;河南省教育厅重点科学研究计划项目

Effect of Exogenous NaHS on Seed Germination and Physiological Characteristics of Cucurbita ficifolia Under NaHCO3 Stress

Yongdong Sun*, Weirong Luo, Xinzheng Li, Guangyin Wang   

  1. School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China
  • Received:2013-04-01 Revised:2013-07-22 Online:2014-01-01 Published:2014-01-01
  • Contact: Yongdong Sun

摘要: 以黑籽南瓜(Cucurbita ficifolia)种子为试材, 研究了外施不同浓度的NaHS对NaHCO3胁迫下种子萌发及生理特性的影响。结果表明, NaHCO3胁迫显著抑制了黑籽南瓜种子的发芽率、胚轴长和胚根长, 降低了种子萌发过程中的可溶性糖含量, 抑制了α-淀粉酶、β-淀粉酶、SOD及POD活性。而外施不同浓度的NaHS显著促进了NaHCO3胁迫下黑籽南瓜萌发种子胚轴和胚根的生长, 提高了可溶性糖含量及α-淀粉酶、β-淀粉酶、SOD和POD活性, 降低了MDA含量; 外施其它盐类(Na2S、Na2SO4、NaHSO4和NaHSO3)及不同pH值(pH5.8–7.8)的Na2HPO4-NaH2PO4缓冲液对NaHCO3胁迫下黑籽南瓜种子的萌发则无影响。外施NaHS可有效缓解NaHCO3胁迫对黑籽南瓜种子萌发的抑制作用, 其缓解效应可能与其释放的H2S有关。

关键词: 黑籽南瓜, NaHS, NaHCO3胁迫, 种子萌发, 生理特性

Abstract: We studied the effect of exogenous NaHS on seed germination and physiological characteristics of Cucurbita ficifolia under NaHCO3 stress. NaHCO3 stress inhibited the germination rate, hypocotyl length and radicle length and decreased the soluble sugar content as well as activities of α-amylase, β-amylase, superoxide dismutase (SOD) and peroxidase (POD). While, exogenous NaHS treatment significantly increased hypocotyl length and radicle length as well as soluble sugar content. Moreover, exogenous NaHS treatment increased the activities of α-amylase, β-amylase, SOD and POD and decreased the accumulation of malondialdehyde (MDA). Treatment with other salts including Na2S, Na2SO4, NaHSO4 and NaHSO3 had no effect on germination rate under NaHCO3 stress. Na2HPO4-NaH2PO4 buffer (pH 5.8–7.8) did not promote seed germination under NaHCO3 stress as did NaHS treatment could be concluded that exogenous NaHS effectively alleviated the toxic effects of NaHCO3 stress on seed germination, and the function of NaHS was attributed to H2S.

Key words: Cucurbita ficifolia, NaHS, NaHCO3 stress, seed germination, physiological characteristics