SUMO E3连接酶在植物生长发育中的功能研究进展

doi:10.11983/CBB17144

摘要/Abstract

摘要： SUMO化是真核生物中一种重要的蛋白质翻译后修饰。SUMO E3连接酶具有对底物特异的识别功能, 可以促进SUMO化反应, 是SUMO化修饰过程中的重要组成部分。目前, 在植物中已经鉴定出多种SUMO E3连接酶, 它们参与植物重要器官的发育调控。该文对植物SUMO E3连接酶在根系发育、开花途径、配子发育和光形态建成中的作用及其调节机制进行综述。

关键词: MMS21, 植物生长发育, SIZ1, SUMO E3连接酶, SUMO化

Abstract: SUMOylation is an important type of protein posttranslational modification. SUMO E3 ligases are critical in this process via substrate recognition and reaction enhancement. Several SUMO E3 ligases, involved in development regulation, have been identified in plants. Here, we summarize the research advances in the functions of SUMO E3 ligases in root development, flowering, gametogenesis and photomorphogenesis and prospects for further study.

Key words: MMS21, plant growth and development, SIZ1, SUMO E3 ligases, SUMOylation

韩丹璐, 赖建彬, 阳成伟. SUMO E3连接酶在植物生长发育中的功能研究进展. 植物学报, 2018, 53(2): 175-184.

Han Danlu, Lai Jianbin, Yang Chengwei. Research Advances in Functions of SUMO E3 Ligases in Plant Growth and Development. Chinese Bulletin of Botany, 2018, 53(2): 175-184.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB17144

https://www.chinbullbotany.com/CN/Y2018/V53/I2/175

图/表 2

图1 植物SUMO E3连接酶的保守结构域(A) 植物SUMO E3连接酶MMS21和SIZ1的特征结构域。SIZ1具有SAP、PHD、PINIT、SP-RING和SXS结构域, MMS21只具有SP-RING结构域(Ishida et al., 2012); (B) SUMO E3连接酶的SP-RING结构域和泛素E3连接酶的RING结构域构型。RING结构域有2个锌离子结合环, SP-RING只含有1个, 第2个环被范德华力和氢键所替代(Duan et al., 2009; Yunus and Lima, 2009; Ishida et al., 2012; 石田喬志和杉本慶子, 2012); (C), (D) 分别为植物SIZ1和MMS21的同源序列比对。数据来源于NCBI。由DNAMAN定义的氨基酸序列、保守序列(黑色)和相似序列(灰色)。SIZ1的蛋白质氨基酸序列为拟南芥(Arabidopsis thaliana) AtSIZ1 (AAU00414.1)、大豆(Glycine max) GmSIZ1a (KRH24918.1)和GmSIZ1b (KRG89029.1)、水稻(Oryza sativa) OsSIZ1 (BAG97182.1)和OsSIZ2 (BAG89374.1)以及玉米(Zea mays) ZmSIZ1a (AQK95338.1)、ZmSIZ1b (AQK79048.1)和ZmSIZc (ONM41936.1)。MMS21的蛋白质氨基酸序列为拟南芥AtMMS21 (NP_188133.2)、大豆GmMMS21 (XP_003541835.1)、水稻OsMMS21 (XP_015640264.1)和玉米ZmMMS21 (AQK88509.1)。

Figure 1 The domains are conserved in plant SUMO E3 ligases(A) Characteristic domains of plant SUMO E3 ligases. MMS21 and SIZ1 are shown by boxes, SIZ1 possesses SAP, PHD, PINIT, SP-RING and SXS domains, while MMS21 possesses only SP-RING domain (Ishida et al., 2012); (B) A schematic model of the SP-RING domain of SUMO E3 ligases and the RING finger of ubiquitin E3 ligases. The RING domain sports two zinc-coordinating loops, the SP-RING domain contains only one, the second loop is instead held together by hydrogen bonds and Van der Waals forces (Duan et al., 2009; Yunus and Lima, 2009; Ishida et al., 2012); (C), (D) The sequence data for the plant SIZ1 homologues were obtained from the NCBI protein database. Sequence identities (black boxes) and similarities (gray boxes) of amino acids were identified by DNAMAN. Amino acid sequences of SIZ1 proteins are from Arabidopsis thaliana AtSIZ1 (AAU00414.1), Glycine max GmSIZ1a (KRH24918.1), GmSIZ1b (KRG89029.1), Oryza sativa OsSIZ1 (BAG97182.1), OsSIZ2 (BAG89374.1), and Zea mays ZmSIZ1a (AQK95338.1), ZmSIZ1b (AQK79048.1), ZmSIZc (ONM41936.1). Amino acid sequences of MMS21 proteins are from Arabidopsis thaliana AtMMS21 (NP_188133.2), Glycine max GmMMS21 (XP_003541835.1), Oryza sativa OsMMS21 (XP_015640264.1), and Zea mays ZmMMS21 (AQK88509.1).

图2 SUMO E3连接酶在拟南芥生长发育中的功能(A) AtMMS21对细胞周期的调节功能(Liu et al., 2016); (B) MMS21和SIZ1对拟南芥开花途径和时间的调控(Kwak et al., 2016); (C) MMS21在拟南芥中响应DNA损伤, 进而影响植物生长发育过程(Xu et al., 2013; Yuan et al., 2014); (D) MMS21通过调节生长素信号途径影响拟南芥根的发育(Ishida et al., 2009; Huang et al., 2009; Zhang et al., 2010); (E) MMS21通过染色质重塑复合物调节根分生组织发育(Zhang et al., 2017a); (F) SIZ1通过调节糖代谢途径和ABA途径影响种子的萌发时间(Miura et al., 2009); (G) SIZ1对开花途径的调节(Jin et al., 2008); (H) SIZ1对植物光形态建成的调控(Kim et al., 2016a; Lin et al., 2016); (I) SIZ1对磷酸饥饿响应的调控(Miura et al., 2005; 2011); (J) SIZ1通过糖代谢途径调节根的发育(Castro et al., 2015)

Figure 2 The functions of SUMO E3 ligases in Arabidopsis(A) The function of MMS21 regulates in cell cycle (Liu et al., 2016); (B) MMS21 and SIZ1 are both involved in flowering pathway and time (Kwak et al., 2016); (C) MMS21 responses to the DNA damage to regulate plant growth and development (Xu et al., 2013; Yuan et al., 2014); (D) MMS21 is involved in Arabidopsis root development by auxin (Ishida et al., 2009; Huang et al., 2009; Zhang et al., 2010); (E) MMS21 is involved in root development by regulating chromatin remodeling complex (Zhang et al., 2017a); (F) SIZ1 regulate glucose metabolism germinated/seedling development (Miura et al., 2009); (G) SIZ1 is involved in flowering pathway (Jin et al., 2008); (H) SIZ1 regulates the photomorphogenesis (Kim et al., 2016a; Lin et al., 2016); (I) SIZ1 regulation of phosphate starvation-induced root architecture remodeling involves the control of auxin accumulation (Miura et al., 2005, 2011); (J) SIZ1 regulates development of root by sugar pathway (Castro et al., 2015)

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