植物学报 ›› 2005, Vol. 22 ›› Issue (04): 439-444.

• 实验简报 • 上一篇    下一篇

普通小麦99-2439 中的白粉病抗性遗传

1牛吉山② 2张丽娜 3 庄丽芳 1王映红   

    1. 1 (河南农业大学国家小麦工程技术研究中心 郑州 450002) 
    2. 2 (西北师范大学生命科学学院 兰州 730070) 
    3. 3 (南京农业大学作物遗传与种质创新国家重点实验室 南京 210095)
  • 收稿日期:2004-06-23 修回日期:2004-12-24 出版日期:2005-08-30 发布日期:2005-08-30
  • 通讯作者: 牛吉山
  • 基金资助:
    河南省杰出青年科学基金(0212000800)和河南省科技厅重点攻关项目(123014400)资助

Inheritance of the Powdery Mildew Resistance in Wheat Line 99-2439

1NIU Ji-Shan② 2ZHANG Li-Na 3ZHUANG Li-Fang 1WANG Ying-Hong   

    1. 1 (National Engineering and Research Center for Wheat, Henan Agricultural University, Zhengzhou 450002) 
    2. 2 (College of Life Science, Northwest Normal University, Lanzhou 730070) 
    3. 3 (State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095)
  • Received:2004-06-23 Revised:2004-12-24 Online:2005-08-30 Published:2005-08-30

摘要: 普通冬小麦品系99-2439在郑州连续4年对田间白粉菌(Blumeria graminis sp. tritici)表现高抗,但其抗性基因来源不清。通过染色体C-分带和1RS染色体特异性SCAR标记鉴定, 表明它是一个小麦-黑麦(Triticum aestivum - Secale cereale)1BL/1RS异易位系。通过对中国春×99-2439杂交F2代分离群 体抗性鉴定和1RS染色体臂检测结果分析, 证明该抗病基因不在1RS染色体臂上。用单孢小麦白粉菌分离株对其抗性遗传进行研究, 结果表明, 99-2439的白粉病抗性由一对小种专化、隐性抗病基因控制。由于携带Pm5a的Hope/8Cc对中国的21个小麦白粉菌分离菌株均高度感病, 而99-2439高抗混和白粉菌和5个单孢分离菌株, 所以, 99-2439所携带的抗白粉病基因不同于Pm5a。

关键词: 小麦, 白粉病, 抗性, 遗传

Abstract: Abstract Common winter wheat (Triticum aestivum) line 99-2439 has shown high resistance to Blumeria graminis sp. tritici in the field in Zhengzhou for 4 years. However, the origin of its powdery mildew resistance genes was unclear. We identified 99-2439 as a Triticum aestivum -Secale cereale 1BL/1RS alien translocation line by chromosome C-banding and 1RS-specific SCAR marker detection. Powdery mildew evaluation and 1RS detection of an F2 segregation population derived from the cross between Chinese Spring wheat and line 99-2439 demonstrated that the powdery mildew resistance gene was located on the 1RS chromosome arm. Inheritance study using a single spore isolate of B. graminis showed that line 99-2439 harbored a racespecific recessive powdery mildew resistance gene. Because 99-2439 was highly resistant to mixed fungi and 5 single spore isolates of B. graminis, the powdery mildew resistance gene of 99- 2439 was different from Pm5a of Hope/8Cc, which was highly susceptible to all 21 isolates of B.graminis in China.

Key words: Wheat (Triticum aestivum), Powdery mildew, Resistance, Inheritance