植物学报 ›› 2019, Vol. 54 ›› Issue (3): 343-349.DOI: 10.11983/CBB18106
收稿日期:
2018-04-26
接受日期:
2018-12-10
出版日期:
2019-05-01
发布日期:
2019-11-24
通讯作者:
包颖
基金资助:
Xia Zhang1,Xiang Jing1,Guangcai Zhou2,Ying Bao1,*()
Received:
2018-04-26
Accepted:
2018-12-10
Online:
2019-05-01
Published:
2019-11-24
Contact:
Ying Bao
摘要: 淀粉作为主要的碳水化合物在储藏能量方面发挥至关重要的作用。颗粒结合型淀粉合酶(GBSS)与直链淀粉的合成息息相关。尽管该酶的编码基因已在许多栽培植物中被分离和确定, 但有关它们在作物野生近缘种中的序列分歧和表达的研究却相对较少。该研究以药用野生稻(Oryza officinalis)为研究对象, 定性和定量地分析了GBSS编码基因的序列特点、与其它植物同源基因的进化关系以及在叶和种子中的表达情况。系统发育分析表明, 该酶在禾本科植物中分别由GBSSI和GBSSII基因编码。在药用野生稻中, 这2种基因所编码蛋白的氨基酸序列一致性为62%, 并且它们在不同器官内呈现时空分化表达, 其中GBSSI在种子中超强表达, GBSSII则主要在叶片表达。
张霞,景翔,周光才,包颖. 药用野生稻GBSS基因的系统发育及组织特异性表达. 植物学报, 2019, 54(3): 343-349.
Xia Zhang,Xiang Jing,Guangcai Zhou,Ying Bao. Phylogeny and Tissue-specific Expression of the GBSS Genes in Oryza officinalis. Chinese Bulletin of Botany, 2019, 54(3): 343-349.
Gene | Primer name | Primer sequence (5'-3') |
---|---|---|
GBSSI | GBSSI-F | AACGTGGCTGCTCCTTGAA |
GBSSI-R | TTGGCAATAAGCCACACACA | |
GBSSII | GBSSII-F | AGGCATCGAGGGTGAGGAG |
GBSSII-R | CCATCTGGCCCACATCTCTA |
表1 GBSSI和GBSSII的引物序列
Table 1 Primer sequences of GBSSI and GBSSII
Gene | Primer name | Primer sequence (5'-3') |
---|---|---|
GBSSI | GBSSI-F | AACGTGGCTGCTCCTTGAA |
GBSSI-R | TTGGCAATAAGCCACACACA | |
GBSSII | GBSSII-F | AGGCATCGAGGGTGAGGAG |
GBSSII-R | CCATCTGGCCCACATCTCTA |
Plants | Gene (GenBank/UniProt No.) | Sequence identity (%) | |
---|---|---|---|
Oryza officinalis | |||
GBSSI | GBSSII | ||
Oryza sativa subsp. japonica | GBSSI (XP_025882300.1) | 95.22 | 60.35 |
GBSSII (XP_015647210.1) | 62.30 | 96.72 | |
O. sativa subsp. indica | GBSSI (AAN77100.1) | 97.87 | 62.30 |
GBSSII (ACY56079.1) | 62.46 | 97.04 | |
Leersia perrieri | GBSSI (A0A0D9WLF6) * | 93.01 | 62.15 |
GBSSII (A0A0D9WED4) * | 58.44 | 85.62 | |
Zizania latifolia | GBSSI (ASSH01051725.1) ** | 90.80 | 62.01 |
GBSSII (ASSH01023543.1) ** | 59.46 | 87.47 | |
Brachypodium distachyon | GBSSI (XP_003557139.1) | 79.31 | 61.16 |
GBSSII (XP_003569238.1) | 60.33 | 85.43 | |
Hordeum vulgare | GBSSI (BAC41202.1) | 81.97 | 60.76 |
GBSSII (BAJ99426.1) | 61.85 | 86.12 | |
Setaria italica | GBSSI (AGW27658.1) | 86.72 | 61.90 |
GBSSII (XP_004956034.1) | 62.40 | 87.34 | |
Sorghum bicolor | GBSSI (XP_002436418.1) | 82.52 | 61.90 |
GBSSII (XP_002461889.1) | 62.62 | 86.18 | |
Triticum aestivum | GBSSI (XP_020146905.1) | 81.37 | 60.36 |
GBSSII (AAG27624.1) | 61.79 | 86.09 | |
Zea mays | GBSSI (NP_001105001) | 82.08 | 62.13 |
GBSSII (NP_001334833) | 62.36 | 85.88 | |
Musa acuminata | GBSS1 (KF512020.1) | 66.39 | 65.35 |
GBSS2 (KF512021.1) | 67.96 | 63.99 | |
GBSS3 (KF512023.1) | 63.93 | 62.91 | |
Arabidopsis thaliana | GBSS (XP_020866584.1) | 62.48 | 63.88 |
Gossypium raimondii | GBSS1 (XP_012474755.1) | 63.89 | 66.01 |
GBSS2 (XP_012439861.1) | 63.11 | 66.77 | |
GBSS3 (XP_012486622.1) | 62.75 | 64.01 | |
Solanum lycopersicum | GBSS (NP_001311457.1) | 67.05 | 62.03 |
Carica papaya | GBSS (XP_021900468.1) | 65.91 | 66.45 |
S. tuberosum | GBSS (XP_006343763.1) | 62.89 | 67.27 |
Vitis vinifera | GBSS1 (XP_010660257.1) | 65.52 | 66.23 |
GBSS2 (XP_019081062.1) | 64.07 | 67.76 | |
Amborella trichopoda | GBSS (XP_006837847.1) | 62.81 | 66.17 |
Chlamydomonas reinhardtii | GBSS (XP_001697117.1) | 47.79 | 47.05 |
表2 药用野生稻和其它植物的GBSS氨基酸序列一致度
Table 2 Amino acid sequence identities of GBSSs between Oryza officinalis and other plants
Plants | Gene (GenBank/UniProt No.) | Sequence identity (%) | |
---|---|---|---|
Oryza officinalis | |||
GBSSI | GBSSII | ||
Oryza sativa subsp. japonica | GBSSI (XP_025882300.1) | 95.22 | 60.35 |
GBSSII (XP_015647210.1) | 62.30 | 96.72 | |
O. sativa subsp. indica | GBSSI (AAN77100.1) | 97.87 | 62.30 |
GBSSII (ACY56079.1) | 62.46 | 97.04 | |
Leersia perrieri | GBSSI (A0A0D9WLF6) * | 93.01 | 62.15 |
GBSSII (A0A0D9WED4) * | 58.44 | 85.62 | |
Zizania latifolia | GBSSI (ASSH01051725.1) ** | 90.80 | 62.01 |
GBSSII (ASSH01023543.1) ** | 59.46 | 87.47 | |
Brachypodium distachyon | GBSSI (XP_003557139.1) | 79.31 | 61.16 |
GBSSII (XP_003569238.1) | 60.33 | 85.43 | |
Hordeum vulgare | GBSSI (BAC41202.1) | 81.97 | 60.76 |
GBSSII (BAJ99426.1) | 61.85 | 86.12 | |
Setaria italica | GBSSI (AGW27658.1) | 86.72 | 61.90 |
GBSSII (XP_004956034.1) | 62.40 | 87.34 | |
Sorghum bicolor | GBSSI (XP_002436418.1) | 82.52 | 61.90 |
GBSSII (XP_002461889.1) | 62.62 | 86.18 | |
Triticum aestivum | GBSSI (XP_020146905.1) | 81.37 | 60.36 |
GBSSII (AAG27624.1) | 61.79 | 86.09 | |
Zea mays | GBSSI (NP_001105001) | 82.08 | 62.13 |
GBSSII (NP_001334833) | 62.36 | 85.88 | |
Musa acuminata | GBSS1 (KF512020.1) | 66.39 | 65.35 |
GBSS2 (KF512021.1) | 67.96 | 63.99 | |
GBSS3 (KF512023.1) | 63.93 | 62.91 | |
Arabidopsis thaliana | GBSS (XP_020866584.1) | 62.48 | 63.88 |
Gossypium raimondii | GBSS1 (XP_012474755.1) | 63.89 | 66.01 |
GBSS2 (XP_012439861.1) | 63.11 | 66.77 | |
GBSS3 (XP_012486622.1) | 62.75 | 64.01 | |
Solanum lycopersicum | GBSS (NP_001311457.1) | 67.05 | 62.03 |
Carica papaya | GBSS (XP_021900468.1) | 65.91 | 66.45 |
S. tuberosum | GBSS (XP_006343763.1) | 62.89 | 67.27 |
Vitis vinifera | GBSS1 (XP_010660257.1) | 65.52 | 66.23 |
GBSS2 (XP_019081062.1) | 64.07 | 67.76 | |
Amborella trichopoda | GBSS (XP_006837847.1) | 62.81 | 66.17 |
Chlamydomonas reinhardtii | GBSS (XP_001697117.1) | 47.79 | 47.05 |
图2 利用氨基酸序列构建的淀粉合酶的最大似然性系统发育树分支旁的数字代表自展支持率。
Figure 2 A maximum likelihood phylogenetic tree of the GBSS based on amino acid sequences Numbers near branches indicate bootstrap value.
图3 GBSSI和GBSSII在药用野生稻叶和种子中的相对表达(A), (B) GBSS基因在叶中的RT-PCR和qRT-PCR扩增结果; (C), (D) GBSS基因在种子中的RT-PCR和qRT-PCR扩增结果。
Figure 3 Relative expression of GBSSI and GBSSII in leaves and seeds of Oryza officinalis(A), (B) Amplification results of RT-PCR and qRT-PCR for GBSS genes in leaves; (C), (D) Amplification results of RT-PCR and qRT-PCR for GBSS genes in seeds.
[1] |
包颖, 杜家潇, 景翔, 徐思 (2015). 药用野生稻叶中淀粉合成酶基因家族的序列分化和特异表达. 植物学报 50, 683-690.
DOI URL |
[2] |
陈凤花, 王琳, 胡丽华 (2005). 实时荧光定量RT-PCR内参基因的选择. 临床检验杂志 23, 393-395.
DOI URL |
[3] |
顾燕娟 (2006). 支链淀粉合成相关基因等位基因间的差异对稻米淀粉理化特性的影响. 硕士论文. 扬州: 扬州大学. pp. 3-8.
DOI URL |
[4] |
王倩, 孙文静, 包颖 (2017). 植物颗粒结合淀粉合酶GBSS基因家族的进化. 植物学报 52, 179-187.
DOI URL |
[5] |
杨学明 (2003). 几个重复序列在不同稻种中的分布及其与稻种分化关系的研究. 硕士论文. 扬州: 扬州大学. pp. 6-10.
DOI URL |
[6] |
张鹏 (2008). 抑制淀粉分支酶类基因表达对稻米品质影响的研究. 硕士论文. 扬州: 扬州大学. pp. 3-8.
DOI URL |
[7] | Bao Y, Xu S, Jing X, Meng L, Qin ZY (2015). De novo assembly and characterization ofOryza officinalis leaf transcriptome by using RNA-seq. Biomed Res Int 2015, 982065. |
[8] |
Cheng J, Khan MA, Qiu WM, Li J, Zhou H, Zhang Q, Guo WW, Zhu TT, Peng JH, Sun FJ, Li SH, Korban SS, Han YP (2012). Diversification of genes encoding granulebound starch synthase in monocots and dicots is marked by multiple genome-wide duplication events.PLoS One 7, e30088.
DOI URL PMID |
[9] |
Dian WM, Jiang HW, Wu P (2005). Evolution and expression analysis of starch synthase III and IV in rice.J Exp Bot 56, 623-632.
DOI URL PMID |
[10] |
Gouy M, Guindon S, Gascuel O (2010). SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building.Mol Biol Evol 27, 221-224.
DOI URL PMID |
[11] |
Guindon S, Gascuel O (2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood.Syst Biol 52, 696-704.
DOI URL PMID |
[12] |
Ohdan T, Francisco Jr PB, Sawada T, Hirose T, Terao T, Satoh H, Nakamura Y (2005). Expression profiling of genes involved in starch synthesis in sink and source organs of rice.J Exp Bot 56, 3229-3244.
DOI URL PMID |
[13] | Shure M, Wessler S, Fedoroff N (1983). Molecular identification and isolation of thewaxy locus in maize. Cell 35, 225-233. |
[14] | Van Harsselaar JK, Lorenz J, Senning M, Sonnewald U, Sonnewald S (2017). Genome-wide analysis of starch metabolism genes in potato (Solanum tuberosum L.). BMC Genomics 18, 37. |
[15] |
Vrinten PL, Nakamura T (2000). Wheat granule-bound starch synthase I and II are encoded by separate genes that are expressed in different tissues.Plant Physiol 122, 255-264.
DOI URL |
[16] | Wang ZY, Wu ZL, Xing YY, Zheng FG, Guo XL, Zhang WG, Hong MM (1990). Nucleotide sequence of ricewaxy gene. Nucleic Acids Res 18, 5898. |
[1] | 李巧峡, 李有龙, 李纪纲, 陈晨龙, 孙坤. 光周期调控维西堇菜与裂叶堇菜开放花和闭锁花的发育[J]. 生物多样性, 2024, 32(6): 23484-. |
[2] | 彭仲韬 金光泽 刘志理. 小兴安岭三种槭树叶性状随植株大小和林冠条件的变异[J]. 植物生态学报, 2024, 48(6): 0-0. |
[3] | 李文博 孙龙 娄虎 于澄 韩宇 胡同欣. 火干扰对兴安落叶松种子萌发的影响[J]. 植物生态学报, 2024, 48(6): 0-0. |
[4] | 袁涵 钟爱文 刘送平 徐磊 彭焱松. 水毛花种子萌发特性的差异及休眠解除方法[J]. 植物生态学报, 2024, 48(5): 638-650. |
[5] | 黄佳慧, 杨惠敏, 陈欣雨, 朱超宇, 江亚楠, 胡程翔, 连锦瑾, 芦涛, 路梅, 张维林, 饶玉春. 水稻突变体pe-1对弱光胁迫的响应机制[J]. 植物学报, 2024, 59(4): 0-0. |
[6] | 杨佳丽, 饶羽菲, 张润花, 周国林, 林处发, 何燕红, 宁国贵. 捕虫堇叶片高效再生体系的建立[J]. 植物学报, 2024, 59(4): 0-0. |
[7] | 臧妙涵, 王传宽, 梁逸娴, 刘逸潇, 上官虹玉, 全先奎. 基于纬度移栽的落叶松叶、枝、根生态化学计量特征对气候变暖的响应[J]. 植物生态学报, 2024, 48(4): 469-482. |
[8] | 梁逸娴, 王传宽, 臧妙涵, 上官虹玉, 刘逸潇, 全先奎. 落叶松径向生长和生物量分配对气候变暖的响应[J]. 植物生态学报, 2024, 48(4): 459-468. |
[9] | 萨其拉, 张霞, 朱琳, 康萨如拉. 长期不同放牧强度下荒漠草原优势种无芒隐子草叶片解剖结构变化[J]. 植物生态学报, 2024, 48(3): 331-340. |
[10] | 朱晓博, 董张, 祝梦瑾, 胡晋, 林程, 陈敏, 关亚静. 重要的种子储存物质长寿命mRNA[J]. 植物学报, 2024, 59(3): 355-372. |
[11] | 范宏坤, 曾涛, 金光泽, 刘志理. 小兴安岭不同生长型阔叶植物叶性状变异及权衡[J]. 植物生态学报, 2024, 48(3): 364-376. |
[12] | 张旋, 徐颖, 杨颜慈, 赵艳玲, 门中华, 王永龙. 孑遗植物半日花叶际真菌群落的多样性与构建机制[J]. 生物多样性, 2024, 32(3): 23384-. |
[13] | 丁扬, 冯英群, 张金羽, 王博. 动物对濒危特有种大别山五针松种子的捕食和散布[J]. 生物多样性, 2024, 32(3): 23401-. |
[14] | 车佳航, 李纬楠, 秦英之, 陈金焕. 木本植物叶色变异机制研究进展[J]. 植物学报, 2024, 59(2): 319-328. |
[15] | 程可心, 杜尧, 李凯航, 王浩臣, 杨艳, 金一, 何晓青. 玉米与叶际微生物组的互作遗传机制[J]. 植物生态学报, 2024, 48(2): 215-228. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||