种子特异性启动子(napinB promoter)分离、表达载体构建及转基因植物获得

植物学报 ›› 2001, Vol. 18 ›› Issue (02): 216-220.

种子特异性启动子(napinB promoter)分离、表达载体构建及转基因植物获得

李丽张景昱杜桂森宋艳茹

１(中国科学院植物研究所北京 100093)２(首都师范大学生物系北京 100037)

收稿日期:2001-01-16 修回日期:2001-02-13 出版日期:2001-03-20 发布日期:2001-03-20
通讯作者: 宋艳茹

Isolation of Seed Specific Promoter (napinB promoter),Construction of Expression Vector and Obtainmentof Transgenic Tobacco Plants

LI Li ZHANG Jing-Yu DU Gui-Sen SONG Yan-Ru

１(Institute　of　Botany,　The　Chinese　Academy　of　Sciences, Beijing　100093)２(Department of Biology，Capital Normal University, Beijing 100037)

Received:2001-01-16 Revised:2001-02-13 Online:2001-03-20 Published:2001-03-20
Contact: SONG Yan-Ru

摘要/Abstract

摘要： 利用PCR技术从油菜Brassica napus H165基因组DNA中分离了napinB启动子。序列分析表明，扩增片段(nap300)与文献报道的napinB启动子相应区域的同源性为97%。将其与gus连接构建种子特异性表达载体，农杆菌介导转化烟草。PCR、Southern结果显示，nap300已整合到烟草基因组DNA中，获得了转基因植株。

Abstract: A fragment of 300bp (nap300) in napinB promter region was isolated by PCR from genomic DNA of Brassica napus H165. Nap300 and the corresponding sequence of napinB promoter shared 97% homology. Expression vector harboring the promoter fused with GUS gene was constructed for functional analysis. Tobacco plants were transformed by Agrobacterium tumefaciens-mediated method. PCR and Southern results showed that nap300 had been integrated into genomic DNA of tobacco successfully. Assay of GUS activity in the seeds of transgenic tobacco will be carried out to identify the function of the promoter.

李丽张景昱杜桂森宋艳茹. 种子特异性启动子(napinB promoter)分离、表达载体构建及转基因植物获得. 植物学报, 2001, 18(02): 216-220.

LI Li ZHANG Jing-Yu DU Gui-Sen SONG Yan-Ru. Isolation of Seed Specific Promoter (napinB promoter),Construction of Expression Vector and Obtainmentof Transgenic Tobacco Plants. Chinese Bulletin of Botany, 2001, 18(02): 216-220.

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