植物学报 ›› 2012, Vol. 47 ›› Issue (5): 462-473.DOI: 10.3724/SP.J.1259.2012.00462 cstr: 32102.14.SP.J.1259.2012.00462
张俊红1,2, 张守攻1, 吴涛1, 韩素英3, 杨文华1, 齐力旺1*
收稿日期:
2012-04-06
修回日期:
2012-05-24
出版日期:
2012-09-01
发布日期:
2012-07-24
通讯作者:
齐力旺
基金资助:
国家自然科学基金项目;国家“973”计划项目
Junhong Zhang1,2, Shougong Zhang1, Tao Wu1, Suying Han3, Wenhua Yang1, Liwang Qi1*
Received:
2012-04-06
Revised:
2012-05-24
Online:
2012-09-01
Published:
2012-07-24
Contact:
Liwang Qi
摘要: 利用同源比对或RACE克隆了5个落叶松(Larix leptolepis) miRNA前体。结果显示, 在各物种miRNA前体间, 成熟序列高度相似, 但其它序列相似度差异大, 序列相似度与亲缘关系有关。采用qRT-PCR分析了5个miRNA、前体和靶基因在落叶松体胚8个发育阶段的表达变化。结果显示, miRNA表达最高峰出现在后期子叶胚, 暗示与促进胚胎休眠有关; 表达次高峰出现时期不同, 表现为miR397和miR408在PEMIII, miR398在早期单胚, miR156和miR166在早期子叶胚, 表明其与保持薄细胞壁、质子传递、顶端分生组织形成等调控有关。miRNA成熟体表达与前体含量不呈线性相关, 可能受多重调控。研究结果对于阐明MIR基因进化、表达调控及在体胚发育中的调控功能具有重要理论意义。
张俊红, 张守攻, 吴涛, 韩素英, 杨文华, 齐力旺. 落叶松体胚发育中5个miRNA前体与成熟体的表达. 植物学报, 2012, 47(5): 462-473.
Junhong Zhang, Shougong Zhang, Tao Wu, Suying Han, Wenhua Yang, Liwang Qi. Expression Analysis of Five MiRNAs and Their Precursors During Somatic Embryogenesis in Larch. Chinese Bulletin of Botany, 2012, 47(5): 462-473.
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