不同枸杞水培条件下不定根发生的转录组比较分析

doi:10.11983/CBB25077

植物学报 ›› 2026, Vol. 61 ›› Issue (3): 462-474.DOI: 10.11983/CBB25077 cstr: 32102.14.CBB25077

不同枸杞水培条件下不定根发生的转录组比较分析

杨改儿¹^,², 张璇¹^,², 王嘉栋¹^,², 张波³, 段淋渊³, 李翔¹^,²^,^*()

¹ 宁夏大学林业与草业学院 , 银川 750021
² 林木资源高效生产全国重点实验室 , 银川 750021
³ 宁夏农林科学院枸杞科学研究所 , 银川 750026

收稿日期:2025-04-25 接受日期:2025-09-02 出版日期:2026-05-10 发布日期:2025-09-03
通讯作者: 李翔
基金资助:
宁夏重点研发计划 (引才专项)(2024BEH04068); 宁夏自然科学基金(2023AAC05025)

Comparative Analysis of Transcriptome of Adventitious Roots Under Different Hydroponic Conditions of Lycium barbarum

Gaier Yang¹^,², Xuan Zhang¹^,², Jiadong Wang¹^,², Bo Zhang³, Linyuan Duan³, Xiang Li¹^,²^,^*()

¹ College of Forestry and Prataculture, Ningxia University , Yinchuan 750021, China
² National Key Laboratory for Efficient Production of Forest Resources , Yinchuan 750021, China
³ Lycium barbarum Research Institute of Ningxia Academy of Agriculture and Forestry Sciences , Yinchuan 750026, China

Received:2025-04-25 Accepted:2025-09-02 Online:2026-05-10 Published:2025-09-03
Contact: Xiang Li

摘要/Abstract

摘要： 枸杞 ( Lycium barbarum) 是重要的经济林树种, 其扦插繁殖效率与不定根形成密切相关, 但枸杞不定根形成相关分子机制尚不明确, 制约了枸杞的规模化繁育及高效利用。以3种生根能力不同的枸杞品系 (L、M和H) 为材料, 通过水培实验分析其不定根发生过程中的转录水平差异。结果显示, 转录组分析鉴定出6 448个差异表达基因 (DEGs), 其中L-vs-H组DEGs数量最多, 达4 413个, 包括2 583个上调和1 830个下调基因。转录因子分析鉴定出281个转录因子, 以MYB、AP2/ERF和bHLH家族为主, 且表达模式存在差异。GO富集分析发现1 714个DEGs被富集到32个GO条目中, KEGG富集分析表明DEGs主要富集于苯丙烷生物合成和植物激素信号转导通路。其中, MYB19 (Lba07g01820) 是苯丙烷通路中的核心基因, TIR1 (Lba08g00069) 是植物激素信号转导通路中的核心基因, 推测它们在枸杞不定根发生过程中发挥重要作用。此外, 通过qRT-PCR验证了转录组数据的可靠性。该研究解析了枸杞不定根形成的转录调控网络, 初步揭示了枸杞不定根形成的分子机理, 为枸杞及其它木本植物的遗传改良提供了新的视角。

关键词: 枸杞, 水培, 不定根发生, 转录组学, 关键基因

Abstract: INTRODUCTION: Lycium barbarum is an important economic forest tree species, and its cutting propagation efficiency is closely related to the formation of adventitious roots. However, the molecular mechanism of adventitious root formation is not clear, which restricts the large-scale breeding and efficient utilization of Lycium barbarum. RATIONALE: In order to explore the transcriptome differences in adventitious root formation of L. barbarum, three wolfberry genotypes with different root-forming abilities were used as experimental materials. A hydroponic experiment was conducted to analyze the transcriptional differences during adventitious root formation. RESULTS: Transcriptome sequencing identified 6 448 differentially expressed genes (DEGs), with the L-vs-H group having the highest number of DEGs at 4 413, including 2 583 upregulated and 1 830 downregulated genes. A total of 281 transcription factors were identified, mainly from the MYB, AP2/ERF, and bHLH families, with distinct expression patterns. GO enrichment analysis revealed that 1 714 DEGs were enriched in 32 GO terms. KEGG enrichment analysis indicated that DEGs were mainly enriched in the phenylpropanoid biosynthesis and plant hormone signal transduction pathways. Among these, MYB19 (Lba07g01820) is a core gene in the phenylpropanoid pathway, and TIR1 (Lba08g00069) is a core gene in the plant hormone signal transduction pathway. Both genes play crucial roles in adventitious root formation in wolfberry. qRT-PCR validated the reliability of the transcriptome data. CONCLUSION: In this study, the transcriptional regulatory network of adventitious root formation in L. barbarum was analyzed, which provided new insights into the root development mechanism of woody plants, revealed the molecular mechanism of adventitious root formation in L. barbarum, and laid an important theoretical foundation for genetic improvement and efficient breeding of L. barbarum and other woody plants.

Key words: wolfberry, hydroponics, adventitious root formation, transcriptomics, key genes

杨改儿, 张璇, 王嘉栋, 张波, 段淋渊, 李翔. 不同枸杞水培条件下不定根发生的转录组比较分析. 植物学报, 2026, 61(3): 462-474.

Gaier Yang, Xuan Zhang, Jiadong Wang, Bo Zhang, Linyuan Duan, Xiang Li. Comparative Analysis of Transcriptome of Adventitious Roots Under Different Hydroponic Conditions of Lycium barbarum. Chinese Bulletin of Botany, 2026, 61(3): 462-474.

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https://www.chinbullbotany.com/CN/Y2026/V61/I3/462

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不同枸杞水培条件下不定根发生的转录组比较分析

Comparative Analysis of Transcriptome of Adventitious Roots Under Different Hydroponic Conditions of Lycium barbarum

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