单倍体育种技术研究进展及其在苜蓿等豆科牧草中的应用

doi:10.11983/CBB22190

植物学报 ›› 2022, Vol. 57 ›› Issue (6): 756-763.DOI: 10.11983/CBB22190

所属专题：饲草生物学专辑 (2023年58卷2期、2022年57卷6期)

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单倍体育种技术研究进展及其在苜蓿等豆科牧草中的应用

王娜¹, 姜腾¹, 王彬锡¹^,², 牛丽芳¹, 林浩¹^,^*()

¹中国农业科学院生物技术研究所, 北京 100081
²河北师范大学, 石家庄 050024

收稿日期:2022-08-09 接受日期:2022-10-09 出版日期:2022-11-01 发布日期:2022-11-18
通讯作者: 林浩
作者简介:*E-mail: linhao@caas.cn
基金资助:
国家重点研发计划(2022YFF1003204);海南省崖州湾种子实验室(B21HJ0215);呼和浩特市“政产学研推用银”创新联合体项目(No.RC2022-联合体-1(RC2022-联合体-1)

Advances in Haploid Breeding Technology and Its Application in Alfalfa and Other Legume Forages

Na Wang¹, Teng Jiang¹, Binxi Wang¹^,², Lifang Niu¹, Hao Lin¹^,^*()

¹Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
²Hebei Normal University, Shijiazhuang 050024, China

Received:2022-08-09 Accepted:2022-10-09 Online:2022-11-01 Published:2022-11-18
Contact: Hao Lin
About author:First author contact:†These authors contributed equally to this paper

摘要/Abstract

摘要： 双单倍体育种通过单倍体诱导与加倍技术相结合实现遗传材料的快速纯合, 显著加快纯系的选育进程和效率, 是农业生物育种中的共性关键技术。该文简要回顾了诱导植物单倍体产生和染色体加倍的相关方法及研究进展, 重点介绍了新近发展的基因编辑介导的体内单倍体诱导技术, 并对单倍体育种技术在紫花苜蓿(Medicago sativa)等豆科牧草中的应用进行了探讨和展望。

关键词: 单倍体育种技术, 基因编辑技术, 豆科牧草, 苜蓿

Abstract: Doubled haploid breeding can achieve rapid homogenization of genetic material through the combination of haploid induction and doubling technology, which significantly accelerates the speed of pure line production and the selection process in hybrid breeding, is a common key technology in agricultural breeding. In this paper, we briefly review the methods and progress of plant haploid induction and chromosome doubling, introduce the latest gene editing-mediated in vivo haploid induction technology, and discuss and prospect the application of haploid breeding technology in Medicago sativa and other legumes forage.

Key words: haploid breeding technology, genome editing technology, legume forage, alfalfa

王娜, 姜腾, 王彬锡, 牛丽芳, 林浩. 单倍体育种技术研究进展及其在苜蓿等豆科牧草中的应用. 植物学报, 2022, 57(6): 756-763.

Na Wang, Teng Jiang, Binxi Wang, Lifang Niu, Hao Lin. Advances in Haploid Breeding Technology and Its Application in Alfalfa and Other Legume Forages. Chinese Bulletin of Botany, 2022, 57(6): 756-763.

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参考文献 55

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目的基因	植物物种	基因编辑类型	单倍体比例(%)	参考文献
AtCENH3	Arabidopsis thaliana	Gene replacement and insertion	25-45	2010
ZmCENH3	Zea mays	Gene replacement and insertion/RNAi	0.16-3.6	2016
TaCENH3	Triticum aestivum	Gene replacement and insertion	7	2020
ZmCENH3	Z. mays	Site-specified knockout	0.5-5	2021
ZmMTL	Z. mays	TALENs	6.7	2017
ZmPLA1	Z. mays	Site-specified knockout	1.55-6.67	2017
ZmNLD	Z. mays	Gene replacement and insertion	0.5-3.59	2017a
OsMATL	Oryza sativa	Site-specified knockout	2-6	2018
TaPLA	T. aestivum	Site-specified knockout	5.88-15.66	2020a
TaMTL	T. aestivum	Site-specified knockout	11.8-31.6	2020b
SiMTL	Setaria italica	Site-specified knockout	1.75-3.49	2021
ZmDMP	Z. mays	Site-specified knockout	0.1-0.3	2019
AtDMP8/9	A. thaliana	Site-specified knockout	0.92-3.23	2020
MtDMP8/9	Medicago truncatula	Site-specified knockout	0.29-0.82	2022
SlDMP	Solanum lycopersicum	Site-specified knockout	0.49-3.68	2022a
BnaDMP	Brassica napus	Site-specified knockout	2.4	2022b
ZmPLD3	Z. mays	Site-specified knockout	0.85-0.96	2021
ZmPOD65	Z. mays	Site-specified knockout	0.9-7.7	2022

目的基因	植物物种	基因编辑类型	单倍体比例(%)	参考文献
AtCENH3	Arabidopsis thaliana	Gene replacement and insertion	25-45	2010
ZmCENH3	Zea mays	Gene replacement and insertion/RNAi	0.16-3.6	2016
TaCENH3	Triticum aestivum	Gene replacement and insertion	7	2020
ZmCENH3	Z. mays	Site-specified knockout	0.5-5	2021
ZmMTL	Z. mays	TALENs	6.7	2017
ZmPLA1	Z. mays	Site-specified knockout	1.55-6.67	2017
ZmNLD	Z. mays	Gene replacement and insertion	0.5-3.59	2017a
OsMATL	Oryza sativa	Site-specified knockout	2-6	2018
TaPLA	T. aestivum	Site-specified knockout	5.88-15.66	2020a
TaMTL	T. aestivum	Site-specified knockout	11.8-31.6	2020b
SiMTL	Setaria italica	Site-specified knockout	1.75-3.49	2021
ZmDMP	Z. mays	Site-specified knockout	0.1-0.3	2019
AtDMP8/9	A. thaliana	Site-specified knockout	0.92-3.23	2020
MtDMP8/9	Medicago truncatula	Site-specified knockout	0.29-0.82	2022
SlDMP	Solanum lycopersicum	Site-specified knockout	0.49-3.68	2022a
BnaDMP	Brassica napus	Site-specified knockout	2.4	2022b
ZmPLD3	Z. mays	Site-specified knockout	0.85-0.96	2021
ZmPOD65	Z. mays	Site-specified knockout	0.9-7.7	2022

单倍体育种技术研究进展及其在苜蓿等豆科牧草中的应用

Advances in Haploid Breeding Technology and Its Application in Alfalfa and Other Legume Forages

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