植物学报 ›› 2022, Vol. 57 ›› Issue (4): 508-531.DOI: 10.11983/CBB22020

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基于CRISPR/Cas9的基因编辑技术研究进展及其在植物中的应用

何晓玲, 刘鹏程, 马伯军, 陈析丰()   

  1. 浙江师范大学化学与生命科学学院, 金华 321004
  • 收稿日期:2022-01-21 修回日期:2022-04-24 出版日期:2022-07-01 发布日期:2022-07-14
  • 通讯作者: 陈析丰
  • 作者简介:第一联系人: 共同第一作者
  • 基金资助:
    国家自然科学基金(31871605);国家自然科学基金(32071987);国家自然科学基金(32101697);浙江省自然科学基金(LD19C130001);浙江省自然科学基金(Q22C133851)

Advance in Gene-editing Technology Based on CRISPR/Cas9 and Its Application in Plants

He Xiaoling, Liu Pengcheng, Ma Bojun, Chen Xifeng()   

  1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
  • Received:2022-01-21 Revised:2022-04-24 Online:2022-07-01 Published:2022-07-14
  • Contact: Chen Xifeng
  • About author:* E-mail: xfchen@zjnu.cn
    First author contact: These authors contributed equally to this paper

摘要: CRISPR/Cas9技术是利用RNA靶向引导Cas9核酸酶对基因组中的目标基因进行编辑的生物技术。近年来, 该技术的多种新型基因编辑器更新迅猛, 编辑效果愈加精细和高效, 在作物定向分子设计育种中展现出巨大的应用前景。该文对CRISPR/Cas9及其相关编辑器的技术原理、编辑效果和应用情况进行综述, 并探讨了该技术在应用中面临的问题、应对措施和发展前景, 旨在为相关领域的科研工作者提供参考。

关键词: CRISPR/Cas9, 基因编辑, 技术进展, 在植物中应用

Abstract: CRISPR/Cas9 technology is a gene editing strategy using Cas9 nuclease guided by RNA to target an interest gene in genome. Recently, a large number of new-type gene editors based on the CRISPR/Cas9 have been updated rapidly and become more precisely and efficiently in gene editing, which has a great application prospect in crop molecular-designing breeding. This paper summarized the technical principles, editing effects and applications of the CRISPR/cas9 and its related gene editors, and also discussed the aspects of dilemmas, countermeasures and prospects, intending to provide reference for scientific researchers in the related fields.

Key words: CRISPR/Cas9, gene editing, technical progress, application in plants