气温和根区温度对葡萄叶片光合荧光特性的影响

doi:10.11983/CBB21122

植物学报 ›› 2022, Vol. 57 ›› Issue (2): 209-216.DOI: 10.11983/CBB21122

气温和根区温度对葡萄叶片光合荧光特性的影响

王浩¹, 王明¹, 梁婷², 姚玉新¹, 杜远鹏¹^,^*(), 高振¹^,^*()

¹山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东果蔬优质高效生产协同创新中心, 泰安 271018
²山东省泰安市岱岳区农业农村局, 泰安 271021

收稿日期:2021-07-25 接受日期:2021-12-28 出版日期:2022-03-01 发布日期:2022-03-24
通讯作者: 杜远鹏,高振
作者简介:gaoz89@sdau.edu.cn
*E-mail: duyuanpeng001@163.com;
基金资助:
国家重点研发计划(2019YFD1000101);财政部和农业农村部和国家现代农业产业技术体系(CARS-29-zp-1)

Effects of High Air and Root Zone Temperature on Photosynthetic Fluorescence Characteristics of Grape Leaves

Hao Wang¹, Ming Wang¹, Ting Liang², Yuxin Yao¹, Yuanpeng Du¹^,^*(), Zhen Gao¹^,^*()

¹Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production in Shandong, State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, China
²Daiyue District Bureau of Agriculture and Rural Areas, Tai’an City, Shandong Province, Tai’an 271021, China

Received:2021-07-25 Accepted:2021-12-28 Online:2022-03-01 Published:2022-03-24
Contact: Yuanpeng Du,Zhen Gao

摘要/Abstract

摘要： 为探究气温和根区温度对葡萄(Vitis vinifera)叶片光合荧光特性的影响, 以一年生巨峰葡萄为试材, 设置对照、高气温、高根区温度和两者交叉作用共4组处理。结果表明, 相较于对照和高气温, 高根区温度以及交叉处理叶片最大光化学效率(F_v/F_m)降低更明显; 与对照相比, 高根区温度以及高气温与高根区温度交叉处理下光系统II (PSII)实际光化学效率Y(II)显著降低, 非调节能量耗散的量子产量Y(NPQ)及Q_A氧化还原状态(1-q_P)值显著上升。同时, 高根区温度以及高气温与高根区温度交叉处理显著增加了J点的可变荧光(V_j), 而用于电子传递的量子产额(φ_Eo)及性能指数(PI_ABS)显著降低。此外, 高根区温度以及高气温与高根区温度交叉处理下单位面积有活性的反应中心数目(RC/CS_m)也显著下降, K点相对可变荧光(W_k)明显上升。综上所述, 高根区温度是高气温与根区高温交叉胁迫的主导因子, PSII受体侧是主要的伤害位点, 高气温加剧了高根区温度对PSII造成的伤害。

关键词: 葡萄, 空气高温, 根区高温, 叶绿素荧光

Abstract: In order to explore the effects of different air and root zone temperature stress on the fluorescence characteristics of grape (Vitis vinifera) leaves, we treated potted annual Kyoho seedlings under four different temperature conditions: control (C), high air temperature (HA), high root zone temperature (HR), and combined treatment (CT) of HA and HR. Our results showed that the maximum photochemical efficiency (F_v/F_m) under HR and CT treatments was more significantly reduced than under C and HA conditions. The actual quantum efficiency Y(II) of the PSII under HR and CT were significantly reduced, while the quantum yield Y(NPQ) of the non-adjusted energy dissipation, and the redox state of Q_A (1-q_p) were significantly increased compared with the control. At the same time, the variable fluorescence (V_j) of the J point was increased significantly under HR and CT treatments, while the quantum yield (φ_Eo) used for electron transfer and the performance index based on absorption of light energy (PI_ABS) were significantly reduced. In addition, the number of active reaction centers per unit area (RC/CS_m) under HR and CT treatments was decreased significantly, while the relative variable fluorescence (W_k) after normalization was increased significantly. Taken together, our study indicated that high temperature in root zone is a key stress affecting leaf fluorescence characteristics by causing damage on the PSII receptor side. High air temperature aggravated the damage on PSII caused by high root zone temperature.

Key words: grapes, high air temperature, high root zone temperature, chlorophyll fluorescence

王浩, 王明, 梁婷, 姚玉新, 杜远鹏, 高振. 气温和根区温度对葡萄叶片光合荧光特性的影响. 植物学报, 2022, 57(2): 209-216.

Hao Wang, Ming Wang, Ting Liang, Yuxin Yao, Yuanpeng Du, Zhen Gao. Effects of High Air and Root Zone Temperature on Photosynthetic Fluorescence Characteristics of Grape Leaves. Chinese Bulletin of Botany, 2022, 57(2): 209-216.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB21122

https://www.chinbullbotany.com/CN/Y2022/V57/I2/209

图/表 4

图1 不同空气温度/根区温度处理对巨峰葡萄叶片Fv/Fm (A)、Y(II) (B)、Y(NPQ) (C)和Y(NO) (D)的影响不同小写字母表示差异显著(P<0.05)。Fv/Fm: PSII最大光化学效率; Y(II): PSII实际光化学量子产量; Y(NPQ): 调节性能量耗散的量子产量; Y(NO): 非调节性能量耗散的量子产量; PAR: 光合有效辐射; CK: 对照; T1: 高气温; T2: 高根区温度; T3: 高气温和高根区温度交叉作用

Figure 1 The influence of different air temperature/root zone temperature treatments on the Fv/Fm (A), Y(II) (B), Y(NPQ) (C) and Y(NO) (D) of Kyoho grape leaves Different lowercase letters indicate significant differences (P<0.05). Fv/Fm: PSII maximum photochemical efficiency; Y(II): PSII actual photochemical quantum yield; Y(NPQ): Regulated energy dissipation quantum yield; Y(NO): Non-regulated energy dissipation quantum yield; PAR: Photosynthetically active radiation; CK: Control; T1: High air temperature; T2: High root zone temperature; T3: Cross processing of high air temperature and high root zone temperature

图2 不同空气温度/根区温度处理对巨峰葡萄叶片Y(CEF) (A)和1-qP (B)的影响不同小写字母表示差异显著(P<0.05)。Y(CEF): 环式电子传递的有效量子产量; 1-qP: QA氧化还原状态; PAR、CK及T1-T3同图1。

Figure 2 The influence of different air temperature/root zone temperature treatments on the Y(CEF) (A) and 1-qP (B) of Kyoho grape leaves Different lowercase letters indicate significant differences (P<0.05). Y(CEF): Circular electron transfer effective quantum yield; 1-qP: QA redox state; PAR, CK and T1-T3 are shown in Figure 1.

图3 不同空气温度/根区温度处理对实际叶绿素荧光诱导曲线(A)和相对叶绿素荧光诱导曲线(B)的影响 CK及T1-T3同图1。

Figure 3 Effect of different air temperature/root zone temperature treatments on the actual chlorophyll fluorescence induction curve (A) and the relative variable chlorophyll fluorescence induction curve (B) CK and T1-T3 are the same as shown in Figure 1.

图4 不同空气和根区温度处理对巨峰葡萄叶片RC/CSm (A)、φEo (B)、Wk (C)、Vj (D)、PIABS (E)和φDo (F)的影响 The effect of different air and root zone temperature treatments on the RC/CSm (A), φEo (B), Wk (C), Vj (D), PIABS (E) and φDo (F) of Kyoho grape leaves不同小写字母表示差异显著(P<0.05)。RC/CSm: 单位面积内有活性的反应中心数量; φEo: 用于电子传递的量子产额; Wk: K点相对可变荧光的变化; Vj: J点的相对可变荧光; PIABS: 性能指数; φDo: 用于热耗散的量子比率; CK及T1-T3同图1。

Figure 4 Different lowercase letters indicate significant differences (P<0.05). RC/CSm: The number of active reaction centers per unit area; φEo: Quantum yield used for electron transfer; Wk: Relatively variable fluorescence of K point; Vj: Relatively variable fluorescence of J point; PIABS: The performance index; φDo: Quantum ratio used for heat dissipation; CK and T1-T3 are shown in Figure 1.

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气温和根区温度对葡萄叶片光合荧光特性的影响

Effects of High Air and Root Zone Temperature on Photosynthetic Fluorescence Characteristics of Grape Leaves

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