植物学报 ›› 2022, Vol. 57 ›› Issue (2): 197-208.DOI: 10.11983/CBB21074

• 研究报告 • 上一篇    下一篇

过表达MtVP1对马铃薯表型及糖代谢的影响

王建武1, 王文娟2, 相微微1, 代惠萍3, 王海庆2, 屈香香1, 亢福仁1,*()   

  1. 1榆林学院陕西省陕北矿区生态修复重点实验室, 榆林 719000
    2中国科学院西北高原生物研究所, 中国科学院高原生物适应与进化重点实验室, 西宁 810008
    3陕西理工大学生物科学与工程学院, 汉中 723001
  • 收稿日期:2021-05-02 接受日期:2021-10-13 出版日期:2022-03-01 发布日期:2022-03-24
  • 通讯作者: 亢福仁
  • 作者简介:*E-mail: kangfuren1987@163.com
  • 基金资助:
    国家自然科学基金(31660075);榆林学院高层次人才科研启动金(14GK39)

Effects of Overexpression of MtVP1 on Potato Phenotypes and Sugar Metabolism

Jianwu Wang1, Wenjuan Wang2, Weiwei Xiang1, Huiping Dai3, Haiqing Wang2, Xiangxiang Qu1, Furen Kang1,*()   

  1. 1Shaanxi Key Laboratory of Ecological Restoration in Shanbei Mining Area, Yulin University, Yulin 719000, China
    2Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
    3College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723001, China
  • Received:2021-05-02 Accepted:2021-10-13 Online:2022-03-01 Published:2022-03-24
  • Contact: Furen Kang

摘要: I型H+-PPase参与糖异生和蔗糖分解代谢, 利用不同的糖(蔗糖、葡萄糖和果糖)饲喂拟南芥(Arabidopsis thaliana) I型H+-PPase基因不同类型的突变体, 产生的表型不一致, 因此, 推测I型H+-PPase可能存在其它影响糖代谢的机制。为进一步明确该酶对糖代谢的影响, 以过表达MtVP1的马铃薯(Solanum tuberosum)渭薯4号为研究对象, 观察不同培养条件下的表型, 监测糖含量变化, 并利用转录组测序分析转录谱。结果表明, 过表达MtVP1马铃薯表现出红色茎、紫色花和表皮毛更发达, 单株块茎数减少, 块茎变大, 块茎皱缩速度加快; 转基因马铃薯块茎中淀粉、葡萄糖和果糖含量显著下降, 芽中葡萄糖和果糖含量也显著下降。果糖饲喂导致转基因马铃薯花青素含量显著降低; 转基因马铃薯体内果糖-1,6-二磷酸酶和果糖- 2,6-二磷酸酶基因表达上调3-7倍。研究结果为进一步从糖代谢角度探究I型H+-PPase的生理功能提供参考。

关键词: I型膜结合H+-PPase, 果糖-1,6-二磷酸酶, 果糖-2,6-二磷酸酶, 糖代谢, 果糖代谢

Abstract: Type I H+-PPase is involved in gluconeogenesis and sucrose catabolism. Different types of mutants of Arabidopsis thaliana type I H+-PPase gene fed with different sugars (sucrose, glucose and fructose) showed various phenotypes. Therefore, it is speculated that type I H+-PPase may contain other mechanisms that affect sugar metabolism. In order to further clarify the effect of the enzyme on sugar metabolism, phenotypes of the MtVP1 overexpressed potato Weishu 4 were observed under different culture conditions. The fluctuation of sugar content was monitored, and the transcription profile was analyzed. The results showed that the MtVP1 overexpressed potato grew red stem, purple flower, and more developed trichomes. Furthermore, tubers per plant decreased in number, but became larger in size, and shriveled up faster. However, the transgenic plant had significantly decreased starch, glucose, and fructose contents in tubers, and glucose and fructose contents in buds. At the same time, feeding fructose significantly reduced the anthocyanin, and up-regulated the gene expression of fructose-1,6-diphosphate and fructose-2,6-diphosphate by 3-7 fold in the transgenic potato. The results could shed light on further studies of the physiological function of type I H+-PPase from the perspective of sugar metabolism.

Key words: type I membrane-associated H+-PPase, fructose-1,6-bisphosphatase, fructose-2,6-bisphosphatase, sugar metabolism, fructose metabolism