不同叶色矢竹叶绿体结构和光系统特性差异

doi:10.11983/CBB17115

植物学报 ›› 2018, Vol. 53 ›› Issue (4): 509-518.DOI: 10.11983/CBB17115

不同叶色矢竹叶绿体结构和光系统特性差异

陈柯伊, 李朝娜, 成敏敏, 赵扬辉, 周明兵, 杨海芸^*()

浙江农林大学, 浙江省竹资源与高效利用协同创新中心, 省部共建亚热带森林培育国家重点实验室, 临安 311300

收稿日期:2017-06-10 接受日期:2017-10-07 出版日期:2018-07-01 发布日期:2018-09-11
通讯作者: 杨海芸
作者简介:† 共同第一作者。
基金资助:
国家自然科学基金(No.31170565)、浙江省自然科学基金(No.LY12C16002)和浙江省分析测试科技计划(No.2016C37081)

Chloroplast Ultrastructure and Chlorophyll Fluorescence Characteristics of Three Cultivars of Pseudosasa japonica

Chen Keyi, Li Zhaona, Cheng Minmin, Zhao Yanghui, Zhou Mingbing, Yang Haiyun^*()

State Key Laboratory of Subtropical Silviculture Zhejiang Provincial Collaborative, Innovation Center for Bamboo Resources and High-Efficiency Utilization, Zhejiang A & F University, Lin’an 311300, China

Received:2017-06-10 Accepted:2017-10-07 Online:2018-07-01 Published:2018-09-11
Contact: Yang Haiyun
About author:† These authors contributed equally to this paper

摘要/Abstract

摘要： 以矢竹(Pseudosasa japonica)、花叶矢竹(P. japonica f. akebonosuji)和曙筋矢竹(P. japonica f. akebono)为研究对象, 借助叶绿体超微结构和荧光动力学曲线的变化揭示不同叶色矢竹的光系统活性及光合特性差异。结果表明: 3个竹种的光合色素含量差异明显, 除花叶矢竹条纹叶白色部分叶绿体内无完整类囊体片层结构外, 花叶矢竹绿条纹和曙筋矢竹的基粒数明显少于矢竹, 叶绿体发育成熟度不一致; OJIP曲线及参数表明, 花叶矢竹条纹绿叶和曙筋矢竹光系统II (PSII)反应中心开放降低程度低于矢竹, 捕获能量用于电子传递的份额变小, PSII活性变弱; 而曙筋矢竹叶片P700至初级电子受体(Q_A)的电子传递链氧化还原平衡偏向于还原侧, 推测其光系统I (PSI)反应中心P700至PSII Q_A电子传递链受损。因此, PSII活性变化导致叶绿体发育不成熟, 可能是引起矢竹类叶色差异的直接原因。

关键词: 叶色变异, 花叶矢竹, 光合色素, 叶绿体超微结构, 叶绿素荧光

Abstract: We explored the different photosynthetic characteristics of three Pseudosasa cultivars: P. japonica, P. japonica f. akebonosuji, and P. japonica f. akebono. The differences in photosystem activity and photosynthetic characteristics of different leaf colors were revealed by the changes of chloroplast ultrastructure and fluorescence kinetics curves. The results showed that the photosynthetic pigment content of the three species was significantly different. Except for the intact thylakoid layer structure in the white part of the chloroplast, the green streak and the radix were significantly less than the radix. The chloroplast developmental maturity is inconsistent; the OJIP curve and parameters indicate that the open reduction of the flowering green leaf and the saplings of the PSII reaction center is lower than that of the yam, the capture energy that is used for the electron transfer share to be smaller, and the PSII activity is weaker; The redox balance of the electron transport chain of bamboo leaves P700 to Q_A is biased towards the reducing side, and it is presumed that the P700 reaction center P700 to PSII primary electron acceptor Q_A electron transport chain is damaged. Therefore, the chloroplast development caused by changes in PSII activity in the photosystem is immature, which may be the direct cause of the difference in leaf color of the species.

Key words: leaf coloration, Pseudosasa japonica f. akebonosuji, photosynthetic pigments, chloroplast ultrastructure, chlorophyll fluorescence

陈柯伊, 李朝娜, 成敏敏, 赵扬辉, 周明兵, 杨海芸. 不同叶色矢竹叶绿体结构和光系统特性差异. 植物学报, 2018, 53(4): 509-518.

Chen Keyi, Li Zhaona, Cheng Minmin, Zhao Yanghui, Zhou Mingbing, Yang Haiyun. Chloroplast Ultrastructure and Chlorophyll Fluorescence Characteristics of Three Cultivars of Pseudosasa japonica. Chinese Bulletin of Botany, 2018, 53(4): 509-518.

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图/表 9

表1 不同种类矢竹叶片光合色素含量及比值(平均值±标准误)

Table 1 Photosynthetic pigments content and relative ratio of different cultivars of Pseudosasa japonica leaves (means±SE)

Photosynthetic pigments	GL	SA	SG	VL
Chla (mg·g^-1FW)	27.19±1.17 a	0.34±0.17 c	25.39±2.41 a	17.09±0.52 b
Chlb (mg·g^-1FW)	8.66±0.33 a	0.16±0.07 c	8.27±0.69 a	5.70±0.17 b
Car (mg·g^-1FW)	4.89±0.28 a	0.32±0.08 c	5.52±0.55 a	4.07±0.13 b
Chla/b	3.14±0.02 a	1.99±0.09 b	3.07±0.03 a	3.00±0.00 a
Chla+b (mg·g^-1FW)	35.86±1.51 a	0.51±0.25 c	33.66±3.11 a	22.79±0.70 b

图1 矢竹类3个叶色叶片表型SG+SA: 花叶矢竹条纹叶片绿色部分及白色部分; VL: 曙筋矢竹淡绿色叶片; GL: 矢竹深绿色叶片

Figure 1 Three kinds of Pseudosasa japonica leavesSA+SG: Albino and green sector in leaf of P. japonica f. akebonosuji with strips; VL: Virescent leaf of P. japonica f. akebono. GL: Green leaf of P. japonica

图2 3个竹种叶片叶绿体结构(A) 花叶矢竹白区叶肉细胞; (B) 花叶矢竹绿区叶肉细胞; (C) 曙筋矢竹叶肉细胞; (D) 矢竹叶肉细胞。G: 基粒; Os: 嗜锇小体; S: 淀粉粒; Th: 类囊体膜

Figure 2 Plate chloroplast ultrastructure of three cultivars of Pseudosasa japonica leaves(A) Mesophyll cells in white zones of zebra leaf of P. japonica f. akebonosuji; (B) Mesophyll cells in green zones of P. japonica f. akebonosuji; (C) Mesophyll cells in the leaf of P. japonica f. akebono; (D) Mesophyll cells in the leaf of P. japonica. G: Granum; Os: Osmiophile globule; S: Starch grain; Th: Thylakoid membranes

图3 不同种类矢竹叶片相对可变荧光强度(Vt)随时间的变化SG、VL和GL同表1。

Figure 3 Relative variable fluorescence (Vt) with the time change of different cultivars of Pseudosasa japonica leavesSG, VL and GL see Table 1.

图4 不同种类矢竹叶片比活性参数VL、GL和SG同表1。ABS/RC: 单位反应中心吸收的光能; TRo/ RC: PSII的最大捕获量; ETo/RC: 单位反应中心用于电子传递的能量; DIo/RC: 单位反应中心的热耗散; ABS/CSo: 单位面积吸收的光能; TRo/CSo: 单位面积捕获的光能; ETo/CSo: 单位面积的电子传递量子产额; DIo/CSo: 单位面积的热耗散; RC/CSo: 单位面积反应中心的数量

Figure 4 Activity parameters for unit reaction center of different cultivars of Pseudosasa japonica leavesVL, GL and SG see Table 1. ABS/RC: The amount of light absorbed by the unit reaction center; TRo/RC: The large amount of PSII; ETo/RC: The energy of the unit reaction center for electron transfer; DIo/RC: The heat dissipation of the unit reaction center; ABS/CSo: Absorption flux per unit area; TRo/CSo: Trapped energy flux per unit area; ETo/CSo: Electron transport flux per unit area; DIo/CSo: Dissipated energy flux perunit area; RC/CSo: Number of active reaction centers per unit area

表2 不同种类矢竹叶片荧光参数分析

Table 2 Analysis of fluorescence parameters of different cultivars of Pseudosasa japonica leaves

	GL	VL	SG
F₀	0.40±0.01 b	0.44±0.03 a	0.39±0.04 ab
F_m	1.64±0.13 a	1.35±0.16 a	1.29±0.17 a
F_v/F_m	0.75±0.02 a	0.67±0.02 b	0.63±0.01 b
F_v/F₀	3.11±0.36 a	2.01±0.19 b	2.26±0.14 b
Y(II)	0.38±0.04 b	0.27±0.03 a	0.31±0.04 a
NPQ	1.27±0.12 b	1.71±0.21 a	1.47±0.18 ab
qP	0.77±0.05 a	0.77±0.03 a	0.79±0.04 a
ETR	23.00±2.94 a	16.22±1.64 a	19.00±2.83 a

图5 3个竹种在不同远红光强度及时间下荧光动力学曲线变化(A1), (A2) 不同远红光强度及时间下花叶矢竹条纹叶片绿色部分荧光动力学曲线(A1)和稳态荧光(A2); (B1), (B2) 不同远红光强度及时间下曙筋矢竹淡绿色叶片荧光动力学曲线(B1)和稳态荧光(B2); (C1), (C2) 不同远红光强度及时间下矢竹深绿色叶片荧光动力学曲线(C1)和稳态荧光(C2)。SG、VL和GL同表1; Ft: 实时荧光曲线。

Figure 5 The change of fluorescence transients under different intensity and time of three cultivars of Pseudosasa japonica leaves under far-red light treatments(A1), (A2) The change of fluorescence transients (A1) and steady-state fluorescence (A2) of green sector in leaf with strips of P. japonica f. akebonosuji under different intensity and time of far-red light treatments; (B1), (B2) The change of fluorescence transients (B1) and steady-state fluorescence (B2) of the virescent leaves of P. japonica f. akebono under different intensity and time of far-red light treatments; (C1), (C2) The change of fluorescence transients (C1) and steady-state fluorescence (C2) of the green leaves of P. japonica under different intensity and time of far-red light treatments. SG, VL and GL see Table 1; Ft: Real-time fluorescence curve.

图6 3个竹种在远红光10-10 (强度-时间)组合下稳态荧光动力学曲线变化SG、VL和GL同表1; Ft同图5。

Figure 6 The change of steady-state fluorescence of 10-10 (intensity-time) of three cultivars of Pseudosasa japonica leaves under far-red light treatmentsSG, VL and GL see Table 1; Ft see Figure 5.

图7 不同远红光强度对3个竹种F0′ (光下最小荧光)的影响SG、VL和GL同表1; Ft同图5; FR: 远红光强度。

Figure 7 The change of F0′ (minimum fluorescence under the light) of three cultivars of Pseudosasa japonica leaves under different intensity of far-red light treatmentsSG, VL and GL see Table 1; Ft see Figure 5; FR: The intensity of far red light.

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不同叶色矢竹叶绿体结构和光系统特性差异

Chloroplast Ultrastructure and Chlorophyll Fluorescence Characteristics of Three Cultivars of Pseudosasa japonica

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