Chin Bull Bot ›› 2018, Vol. 53 ›› Issue (4): 502-508.doi: 10.11983/CBB17097

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

The Effects of Different Phosphor Excitation-type LED Lighting on the Growth and Development of Arabidopsis

Zhou Zhi1,2,3, Zhang Yongli2, Wang Bianbian2, Gai Shujie1,2, Wang Ruozhong3, Lu Xiangyang3, Li Ruilian1,*()   

  1. 1College of Agriculture, Hunan Agricultural University, Changsha 410128, China
    2College of Science, Hunan Agricultural University, Changsha 410128, China
    3College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
  • Received:2017-05-16 Accepted:2017-12-26 Online:2018-09-11 Published:2018-07-01
  • Contact: Li Ruilian E-mail:2323662303@qq.com
  • About author:† These authors contributed equally to this paper

Abstract:

To investigate the effects of different red and blue bands on the growth and development of Arabidopsis, we used the model plant Arabidopsis thaliana Columbia-0 to observe the relation between light quality and plant growth. The phosphor excitation-type LED lighting affords an adjustable light spectrum by adjusting the amount of the nitride-based phosphor SrSiAlN3:Eu2+. The germination rate, root length, variation in plant height, chlorophyll content, conductivity and other parameters of the model plant under different light spectra were studied. The germination rate could reach 95.63% and the content of chlorophyll by SPAD meter was up to 26.7 with a red to blue band ratio of 2:1. Root length was much longer and could even reach 4.19 cm under a ratio of 4:5. The height showed the highest value of 15.5 cm with a red to blue band ratio of 4:1, and conductivity was as high as 40.5 S·m-1 with a ratio of 4:5. Maintaining the relative balance of red and blue light can have a positive effect on growth of Arabidopsis, and decreasing the ratio of blue light will benefit the growth of roots and accumulation of chlorophyll. Different light spectra have a great influence on the growth and deve- lopment of the model plant Arabidopsis, and the spectral adjustments can be one of the most important environmental factors for plant research.

Key words: phosphor excitation-type LED lighting, plant growth, model plant, spectral form

Figure 1

The scanning electron microscope (SEM) and physical photo of phosphor SrSiAlN3:Eu2+"

Figure 2

The diagram of experimental device (A) and the photos of whole (B) and individual (C) Arabidopsis plants under different light conditions"

Figure 3

The XRD diffraction pattern of phosphor sample SrSiAlN3:Eu2+"

Figure 4

The PL and PLE spectrum pattern of phosphor SrSiAlN3:Eu2+"

Table 1

Effect of different light quality on the Arabidopsis seed germination rate"

Spectrum Day 3 (%) Day 4 (%) Day 5 (%) Day 6 (%) Day 7 (%) Day 8 (%)
CK (WET) 23.90 60.78 82.33 89.26 90.42 91.73
T1 (RED(610)) 25.39 65.39 89.41 90.21 90.31 92.26
T2 (R(610)4B1) 25.13 62.09 89.52 90.36 92.33 95.05
T3 (R(610)2B1) 26.67 65.52 90.74 91.74 94.25 95.63
T4 (R(610)4B5) 24.90 64.37 85.26 92.36 94.31 93.45
T5 (BLUE(460)) 23.85 62.37 80.26 89.63 91.42 90.39

Table 2

The effects of different light quality index on the growth of Arabidopsis thaliana"

Spectrum Root length (cm) Grass height (cm) Chlorophyll content (SPAD) Conductivity (S·m-1)
CK (WET) 3.27 cCD 12.32 cdC 25.19 bB 38.91 aA
T1 (RED(610)) 3.00 cD 11.34 dC 23.32 cCD 37.89 aA
T2 (R(610)4B1) 3.78 aAB 15.48 aA 26.69 aA 39.78 aA
T3 (R(610)2B1) 3.67 bBC 13.94 bB 23.88 cC 40.10 aA
T4 (R(610)4B5) 4.19 aA 12.63 cBC 22.43 dD 40.50 aA
T5 (BLUE(460)) 3.02 cD 12.22 cdC 20.32 eE 37.42 aA
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