Chin Bull Bot ›› 2013, Vol. 48 ›› Issue (1): 34-41.doi: 10.3724/SP.J.1259.2013.00034

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Isolation and Functional Characterization of Heat-stressresponsive Gene TaWTF1 from Wheat

Dandan Qin1,2, Songchao Xie2, Gang Liu2, Zhongfu Ni2, Yingyin Yao2, Qixin Sun2,3, Huiru Peng2*   

  1. 1Institute of Food Crops, Hubei Academy of Agricultural Science, Wuhan 430064, China;

    2China Agricultural University, Beijing 100193, China;

    3Northwest Agriculture and Forest University, Yangling 712100, China
  • Received:2012-05-22 Revised:2012-07-26 Online:2012-11-01 Published:2013-01-01
  • Contact: Huiru Peng

Abstract: What’s this factor 1 genes (WTF1s) encode a group of proteins with Domain of Unknown Function 860 which are localized specifically in the plant chloroplast and mitochondria and play an important role in intron splicing. Previously, a heat-responsive probe with this conserved domain was identified in the Affymetrix Wheat Genome Array. With the probe sequence, the open reading frame was cloned from wheat and was named TaWTF1. Bioinformatics analysis showed that the promoter of TaWTF1 contained some stress- and hormone-responsive elements including a heat-shock element. TaWTF1 gene expression was induced by heat treatments at the seedling and flowering stages. Moreover, the expression of this gene was higher in the common leaves. Importantly, overexpression of TaWTF1 improved the survival rate of Arabidopsis under heat stress. This study provides valuable information to understand more about the molecular mechanism of thermotolerance in plants.

[1] Francs-Small CC, Kroeger T, Zmudjak M, Ostersetzer-Biran O, Rahimi N, Small I, Barkan A (2011). A PORR domain protein required for rpl2 and ccmFc intron splicing and for the biogenesis of c-type cytochromes in Arabidopsis mitochondria. The Plant J (Early edition)
[2] Konishi M, Sugiyama M (2006). A Novel Plant-Specific Family Gene, ROOT PRIMORDIUM DEFECTIVE 1, Is Required for the Maintenance of Active Cell Proliferation. Plant Physiol 140, 591-602
[3] Qin DD, Wu HY, Peng HR, Ni ZF, Yao YY, Li ZX, Zhou CL, Sun QX (2008). Heat stress-responsive transcriptome analysis in heat susceptible and tolerant wheat (Triticum aestivum L.) by using Wheat Genome Array. BMC Genomics 9, 432
[4] Song L, Sultan C, Jeffery H, John C, Ron M (2008). Enhanced tolerance to oxidative stress in transgenic Arabidopsis thaliana plants expressing proteins of unknown function. Plant Physiol 148, 280-292
[5] Tiffany SK, Kenneth PW, Giulia F, Klaas JW, Alice B (2009). A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. PNAS 106, 4537-4542
[6] 秦丹丹 (2008). 小麦苗期热胁迫转录谱分析及相关基因的克隆和功能鉴定. 博士论文. 北京:中国农业大学. pp. 19-51
[7] 张庆波 (2009). 植物杂交种和亲本基因差异表达与mRNA稳定性和可变剪接的关系研究. 博士论文. 北京:中国农业大学. pp. 39
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[1] Longbiao Guo;Chengcai Chu;Qian Qian. Rice Mutants and Functional Genomics[J]. Chin Bull Bot, 2006, 23(1): 1 -13 .
[2] Wang Tai;Tong Zhe and Zhao Yu-jing. Photoperiod-regulation of the Activity of Ascorbate Peroxidase in leaves of Photoperiod Sensitive Genic Male Sterile Rice[J]. Chin Bull Bot, 1993, 10(03): 36 -40 .
[3] Yi Sun, Hui Li, Liangsheng Wang, Silan Dai. Rapid, Effective Method for Anthocyanin Analysis in Tobacco Corolla[J]. Chin Bull Bot, 2011, 46(2): 189 -196 .
[4] Wu Zhao-ming Wang Quan-qiu Gao Xiao-xia and Xu Rong. Effect of Lanthanum and Cerium on the Activity of Glutamic-Pyruvic Transminase and Glutamate Dehydrogenase[J]. Chin Bull Bot, 1997, 14(增刊): 64 -67 .
[5] Guisheng Li;Mingsheng Chen. Flowers, Genes, and Poaceae[J]. Chin Bull Bot, 2007, 24(01): 42 -48 .
[6] WU Min-Sheng and DAI Jing-Rui. Amplified Fragment Length Polymorphism (AFLP) a New Molecular Marker[J]. Chin Bull Bot, 1998, 15(04): 68 -74 .
[7] . [J]. Chin Bull Bot, 1998, 15(专辑): 39 -40 .
[8] Jiang Shu. An Introduction on Advances of Botany in Japan[J]. Chin Bull Bot, 1984, 2(01): 67 -70 .
[9] Zifen Wang;Yi Ren. Advances in the Study of the Angiosperm Ovule[J]. Chin Bull Bot, 2007, 24(01): 49 -59 .
[10] Chang Jie Ge Ying Li Gang. Quantative Relation Between the Physiecological Process of Bean and Spectrum[J]. Chin Bull Bot, 1995, 12(专辑2): 109 -116 .