Advance in Molecular Mechanism of MBF1 Regulating Plant Heat Response and Development

doi:10.11983/CBB21220

Abstract

Abstract: MBF1 (multiprotein bridging factor 1) is a transcriptional co-activator, which is highly conserved in evolution. It exists in all eukaryotes and can activate gene transcription by connecting components of the basal transcription machinery and transcription factors. Plant MBF1 plays important roles in a variety of biological processes, including controlling plant growth and development, and adversity adaptation. This review demonstrates the advance in the molecular structure and regulation mechanism of plant MBF1, and highlights the molecular mechanism of AtMBF1c in the regulation of plant heat stress response.

Key words: heat stress, MBF1, plant, transcriptional regulation

Yi Qin, Yanshuang Liu, Liuliu Qiu, Min Zhou, Xiaoshan Du, Shaojun Dai, Meihong Sun. Advance in Molecular Mechanism of MBF1 Regulating Plant Heat Response and Development[J]. Chinese Bulletin of Botany, 2022, 57(1): 56-68.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB21220

https://www.chinbullbotany.com/EN/Y2022/V57/I1/56

Figures/Tables 4

Table 1 Members of the MBF1 protein family in different plant species

物种	拉丁名	蛋白名	蛋白编号(JGI数据库)	参考文献
拟南芥	Arabidopsis thaliana	AtMBF1a	AT2G42680.1	Tsuda et al., 2004
		AtMBF1b	AT3G58680.1
		AtMBF1c	AT3G24500.1
番茄	Solanum lycopersicum	SlMBF1a	Solyc10g007350.3.1	Zhang et al., 2019
		SlMBF1b	Solyc12g014290.2.1
		SlMBF1c	Solyc07g062400.3.1
		SlMBF1d	Solyc09g055470.1.1
		SlER24	Solyc01g104740.3.1
水稻	Oryza sativa	OsMBF1a	LOC_Os08g27850.1	Zhang et al., 2019
水稻	Oryza sativa	OsMBF1c	LOC_Os06g39240.1	Zhang et al., 2019
菠菜	Spinacia oleracea	SoMBF1b	Spov3_C0009.00073	Xu et al., 2017
菠菜	Spinacia oleracea	SoMBF1c	Spov3_C0062.00022	Xu et al., 2017
马铃薯	S. tuberosum	StMBF1a	PGSC0003DMP400012592	Yu et al., 2021
		StMBF1b	PGSC0003DMP400026892
		StMBF1c	PGSC0003DMP400051869
		StMBF1d	PGSC0003DMP400051868
小麦	Triticum aestivum	TaMBF1a-1	Traes_2AL_A9D390619.1	Qin et al., 2015; Tian et al., 2021
		TaMBF1a-2	Traes_2BL_4F31B5695.1
		TaMBF1a-3	Traes_2DL_D4AB94C53.1
		TaMBF1a-4	Traes_3AS_719D37CCA.1
		TaMBF1a-5	Traes_3B_EC2B74116.1
		TaMBF1b	Traes_4DS_F1C77E7B0.1
		TaMBF1c-7A	Traes_7AL_FA77CC1F41.1
		TaMBF1c-7B	Traes_7BL_A002364C5.1
		TaMBF1c-7C	Traes_7DL_D5AD8EB4B.1
葡萄	Vitis vinifera	VvMBF1a	VIT_212s0028g02020.1	Yan et al., 2014
		VvMBF1a-like	VIT_219s0014g01260.1
		VvMBF1c	VIT_211s0016g04080.1
大豆	Glycine max	GmMBF1a-1	Glyma.06G276200.1.p	Tsuda et al., 2004
		GmMBF1a-2	Glyma.06G276300.1.p
		GmMBF1a-3	Glyma.12G129100.1.p
玉米	Zea mays	ZmMBF1a	ZmPHB47.01G345100.1.p	Tsuda et al., 2004
		ZmMBF1b	ZmPHB47.04G054300.1.p
		ZmMBF1c	ZmPHB47.09G119100.1.p
蓖麻	Ricinus communis	RcMBF1b	27894.m000799	Tsuda et al., 2004
蓖麻	Ricinus communis	RcMBF1c	29912.m005549	Tsuda et al., 2004
蒺藜苜蓿	Medicago truncatula	MtMBF1b-1	Medtr2g084220.1	Tsuda et al., 2004
		MtMBF1b-2	Medtr4g080090.1
		MtMBF1b-3	Medtr6g018330.1
		MtMBF1c	Medtr6g086280.1

Figure 1 The analysis of the phylogenetic relationships, gene structures and conserved motifs of plant MBF1 (A) Phylogenetic relationship, gene structure and conserved motif distribution map of plant MBF1. The full-length protein sequences of MBF1 proteins from 11 species are downloaded from the JGI database (https://phytozome-next.jgi.doe.gov/) (table 1). MEGA7.0 software is used to construct a Neighbor-Joining homologous phylogenetic tree. The green background indicates the type I subfamily, and the red background indicates the type II subfamily. GSDS2.0 software (http://gsds.cbi.pku.edu.cn/) is used to draw a gene structure diagram online. 5'UTR/3'UTR are represented by blue boxes, exons are represented by yellow boxes, and introns are represented by black straight lines. The MEME website (https://meme-suite.org/meme/tools/meme) is used to predict conserved motifs. Conserved domains and functions are identified through the NCBI website CDD database (https://www.ncbi.nlm.nih.gov/cdd). Tbtools software is used to draw a map of the conserved motifs, and the 5 conserved motifs are indicated by boxes with different colors. (B) The predicted conserved motif sequences in MBF1 proteins of 11 species.

Figure 2 Homologous sequence alignment analysis of MBF1s of different species The protein sequences of Bombyx mori BmMBF1 and Saccharomyces cerevisiae yMBF1 are downloaded from Ensembl database (https://ensemblgenomes.org/), the corresponding protein accession number is BGIBMGA007702 and YOR298C-A. DNAMAN software is used for sequence alignment. The purple box indicates the N-terminal MBF1 domain, and the blue box indicates the C-terminal HTH_3 domain. The protein sequences and protein accession number of Arabidopsis and rice MBF1 are listed in Table 1.

Figure 3 Signaling pathway of MBF1 regulating heat stress response Heat stress causes Ca2+ influx by activating the plasma membrane-localized protein CNGC2, and leads to the accumulation of ROS by activating the plasma membrane-bound RBOHD. The Ca2+ signal and ROS signal activate the heat stress response by regulating AtMBF1c and its downstream target genes through unknown pathways. The heat stress transcription factor HSFA1 interacts with HSFA2 and directly regulates the expression of AtMBF1c, HSFA2, HSFBs and DREB2A. At the same time, AtMBF1c binds to the HSE elements of DREB2A, HSFB2A and HSFB2B promoters to regulate their gene expression and improve heat stress tolerance. DREB2A interacts with the trimeric co-activation complex formed by DPB3-1, NF-Y A2 and NF-Y B3 to enhance the transcriptional activation of the downstream target gene HSFA3 and improve plant heat tolerance. DREB2A also promotes the expression of HSP70, HSP18.2 and At1g52560 to enhance plant heat tolerance. As the upstream regulator, AtSAP5 interacts with and activates AtMBF1c in the nucleus, regulating the expression of HSP18.2 and improving plant heat tolerance. Heat stress induces the expression of TPS5. AtMBF1c interacts with TPS5 to improve heat tolerance by promoting the synthesis and accumulation of trehalose. Heat stress induces the expression of AtWRKY39, AtWRKY25, AtWRKY26 and AtWRKY33. AtWRKY39 regulates the expression of the downstream gene of salicylic acid (SA) signaling pathway PR1 through AtMBF1c to improve heat tolerance. AtWRKY25, AtWRKY26 and AtWRKY33 regulate the expression of downstream genes in the ethylene (ET) signaling pathway through AtMBF1c to improve heat resistance, and at the same time promote the expression of HSP70, HSP101, HSFA2, and HSFB1. CNGC2: Cyclic nucleotide-gated channel 2; DPB3-1 (NF-YC10): DNA polymerase II subunit B3-1; DRE: Dehydration-responsive element; DREB2A: Dehydration responsive element-binding protein 2A; HSE: Heat shock elements; HSFA1/2/3: Heat stress transcription factor A1/2/3; HSFB2A/B: Heat stress transcription factor B2A/B; HSP18.2/ 70/101: Heat shock protein 18.2/70/101; MBF1c: Multiprotein bridging factor 1c; NF-Y A2/B3: Nuclear factor Y A2/B3; PR1: Pathogenesis-related factor 1; RBOHD: Respiratory burst oxidase homologue D; ROS: Reactive oxygen species; SAP5: Stress-associated protein 5; TPS5: Trehalose phosphate synthetase 5. The blue solid arrow indicates protein interaction; the green solid arrow indicates gene encoding protein; the black solid arrow indicates direct transcription activation; the black dashed arrow indicates indirect transcription activation.

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