Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (4): 535-547.DOI: 10.11983/CBB22081

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Evaluation and Genetic Variation of Grain Lutein Contents in Common Wheat From Shanxi

Bangbang Wu1, Yuqiong Hao2, Shubin Yang1, Yuxi Huang1, Panfeng Guan3, Xingwei Zheng2, Jiajia Zhao2, Ling Qiao2, Xiaohua Li2, Weizhong Liu1(), Jun Zheng2()   

  1. 1. College of Life Sciences, Shanxi Normal University, Taiyuan 030031, China
    2. Key Laboratory of Organic Dry Farming Agriculture of the Ministry of Agriculture and Rural Affairs (Jointly Built by Provinces and Departments), Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, China
    3. School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China
  • Received:2022-04-20 Accepted:2022-09-19 Online:2023-07-01 Published:2022-11-11
  • Contact: *E-mail:;
  • About author:† These authors contributed equally to this paper.

Abstract: Lutein can effectively reduce incidence of atherosclerosis, diabetes, cancer and multiple eye diseases. The lutein biofortification through food crop has gained more attention with improvement of daily diet. In this paper, 194 Shanxi wheat cultivars planted in three environments were used to extract lutein by organic solvent extraction, and the content of lutein in different germplasms was determined by high performance liquid chromatography (HPLC). The broad-sense heritability of lutein content in wheat and its relationship with grain color, winter/spring types, geographical origin, accession types, and main agronomic traits were analyzed, and the genetic loci associated with lutein content were identified through genome-wide association analysis. Results showed that significant variation in lutein contents occurred among Shanxi wheat accessions, the coefficient of variation was 33.12%-48.57%. Genotype was the main factor affecting lutein content. The average lutein content in three environments was 0.67-4.03 μg·g-1, 0.16-5.05 μg·g-1 and 0.16-3.63 μg·g-1, respectively. The average lutein content of winter types and irrigated-wheat accessions were higher than those of spring types and dryland-wheat, respectively. There was no significant effect of grain color and released years on lutein content. Heading date, plant height and 1 000 kernel weight were significantly negative correlated with lutein content. The other agronomic traits had no significant effect on lutein. Genome-wide association analysis found four major loci related to lutein content on chromosomes 1B, 3A and 7A, among them, QLuc.3A and QLuc.7A.1 are new loci affecting the lutein content. These results provide valuable information for breeding and cultivation of wheat lutein bioaugmentation varieties.

Key words: wheat, lutein, genetic factor, agronomic traits, genome-wide association study