植物学报 ›› 2020, Vol. 55 ›› Issue (1): 90-95.DOI: 10.11983/CBB19168

• 技术方法 • 上一篇    下一篇

怀牛膝细胞悬浮培养条件的优化

李萍1,2,董亚辉1,李成龙1,何雨龙1,李明军1,3,*()   

  1. 1河南师范大学生命科学学院, 新乡 453007
    2郑州澍青医学高等专科学校, 郑州 450064
    3河南省道地中药材保育及利用工程技术研究中心/绿色药材生物技术河南省工程实验室, 新乡 453007
  • 收稿日期:2019-08-27 接受日期:2019-11-13 出版日期:2020-01-01 发布日期:2019-12-20
  • 通讯作者: 李明军
  • 基金资助:
    国家中医药行业科研专项子课题(201407005-08);河南省创新型科技人才队伍建设工程(C20130037);河南省高等学校青年骨干教师培养计划(2018GGJS293)

Optimization of Cell Suspension Culture Conditions of Achyranthes bidentata

Ping Li1,2,Yahui Dong1,Chenglong Li1,Yulong He1,Mingjun Li1,3,*()   

  1. 1College of Life Science, Henan Normal University, Xinxiang 453007, China
    2Zhengzhou Shuqing Medical College, Zhengzhou 450064, China
    3Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province/Engineering Laboratory of Biotechnology for Green Medicinal Plant of Henan Province, Xinxiang 453007, China
  • Received:2019-08-27 Accepted:2019-11-13 Online:2020-01-01 Published:2019-12-20
  • Contact: Mingjun Li

摘要: 为进一步优化怀牛膝(Achyranthes bidentata)细胞悬浮培养条件, 对接种量、继代周期、pH、光照及Cu 2+等多种影响因子的作用效果进行了研究, 以提高怀牛膝细胞生长量及牛膝多糖含量。结果显示, 接种量50 g·L -1、继代周期14天, pH5-6和光照培养可以使细胞保持良好的生长状态及多糖合成能力; 添加50 μmol·L -1Cu 2+, 细胞的干重最大, 可达44.63 g·L -1, 多糖含量也最高, 为4.02 mg·g -1

关键词: 怀牛膝, 细胞悬浮培养, 细胞生长, 多糖

Abstract: To optimize the suspension culture conditions of Achyranthes bidentata cells, effect of different factors like inoculum concentrations, subculture cycles, pH, light and Cu 2+ on cell growth and polysaccharides contents was studied. The results showed that, cells grew well with a good ability for polysaccharides synthesis under 50 g·L -1 inoculum concentration, 14 d subculture cycle, pH5-6 and light. In the presence of 50 μmol·L -1 Cu 2+, the cell dry weight could be the maximum with 44.63 g·L -1, and polysaccharides contents reached the peak at 4.02 mg·g -1.

Key words: Achyranthes bidentata, cell suspension culture, cell growth, polysaccharides