转基因红花中角质细胞生长因子KGF-1的表达

doi:10.3724/SP.J.1259.2011.00311

植物学报 ›› 2011, Vol. 46 ›› Issue (3): 311-318.DOI: 10.3724/SP.J.1259.2011.00311

转基因红花中角质细胞生长因子KGF-1的表达

江莺^1,2, 刘秀明^1,3, 马吉胜¹, 李巍^1,3, 朱海林^1,3, 杜美丽^1,3, 李海燕^1,3*, 李校堃^1*

¹吉林农业大学生物反应器与药物开发教育部工程研究中心, 长春 130118;
²吉林农业大学中药材学院, 长春 130118
³吉林农业大学生命科学学院, 长春 130118

收稿日期:2010-12-01 修回日期:2011-01-24 出版日期:2011-05-01 发布日期:2011-05-26
通讯作者: 李海燕,李校堃
基金资助:
国家高技术研究发展计划(863计划);吉林省科技发展计划重点项目;高等学校科技创新工程重大项目培育资金项目

Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower

Ying Jiang^1,2, Xiuming Liu^1,3, Jisheng Ma¹, Wei Li^1,3, Hailin Zhu^1,3, Meili Du^1,3, Haiyan Li^1,3*, Xiaokun Li^1*

¹Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University, Changchun 130118, China;

²College of Chinese Medicinal, Jilin Agricultural University,Changchun 130118, China;

³College of Life Sciences, Jilin Agricultural University, Changchun 130118, China

Received:2010-12-01 Revised:2011-01-24 Online:2011-05-01 Published:2011-05-26
Contact: Haiyan Li,Xiaokun Li
Supported by:
National High Technology Research and Development Program of China (863 Program);the Science and Technology Development Projects of Jinlin Province;Important Projects in the Scientific Innovation of Colleges and Universities in China

摘要/Abstract

摘要： 通过构建重组表达质粒载体p139035S-KGF1和根癌农杆菌介导在红花(Carthamus tinctorius)中表达角质细胞生长因子(KGF-1)。从侵染到诱导生根共需要14周, 转化率达0.1%。红花子叶在潮霉素筛选培养基上培养4–5周后便可获得丛生芽, 再生芽移入含潮霉素的伸长生根培养基, 培养4–8周可诱导生根。通过PCR、Southern blot、RT-PCR及Western blot检测证明目的基因KGF-1已经整合到红花细胞的染色体中, 实现了KGF-1外源蛋白在红花中的成功表达, 为开发KGF-1蛋白新的生产途径奠定了基础。

Abstract: The recombinant plasmid vector p139035S-KGF1 was constructed and transfected into safflower by Agrobacterium tumefaciens transfection. The period of infection to rooting was 14 weeks, and transformation efficiency was about 0.1%. Adventitious shoots were generated after cotyledons were cultured in selecting media containing hygromycin for 4–5 weeks. Rooted safflower was obtained in 4–8 weeks. KGF-1 was transformed into safflower chromosomes and expressed successfully, as determined by PCR, Southern blot, RT-PCR and Western blot analysis, which established the basis for the production of KGF-1.

江莺, 刘秀明, 马吉胜, 李巍, 朱海林, 杜美丽, 李海燕, 李校堃. 转基因红花中角质细胞生长因子KGF-1的表达. 植物学报, 2011, 46(3): 311-318.

Ying Jiang, Xiuming Liu, Jisheng Ma, Wei Li, Hailin Zhu, Meili Du. Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower. Chinese Bulletin of Botany, 2011, 46(3): 311-318.

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转基因红花中角质细胞生长因子KGF-1的表达

Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower

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