植物学报 ›› 2011, Vol. 46 ›› Issue (3): 302-310.DOI: 10.3724/SP.J.1259.2011.00302

• 研究报告 • 上一篇    下一篇

利用离体叶片鉴定杨树耐盐潜力

张颖1, 杨迎霞1, 郏艳红2, 周祥明2, 聂莉莉2, 张越2, 陈受宜3, 王景安1*, 刘仲齐2*   

  1. 1天津师范大学生命科学学院, 天津 300384;
    2天津市农业生物技术研究中心, 天津 300192
    3中国科学院遗传与发育生物学研究所, 北京 100101
  • 收稿日期:2010-10-08 修回日期:2011-01-21 出版日期:2011-05-01 发布日期:2011-05-26
  • 通讯作者: 王景安,刘仲齐
  • 基金资助:

    国家重点基础研究发展计划;“十一五”国家科技支撑计划

Using Leaf Sections to Identify Different Salt Tolerance Potential of Poplar Plants

Ying Zhang1, Yingxia Yang1, Yanhong Jia2, Xiangming Zhou2, Lili Nie2, Yue Zhang2, Shouyi Chen3, Jingan Wang1*, Zhongqi Liu2*   

  1. 1College of Life Sciences, Tianjin Normal University, Tianjin 300384, China;

    2Tianjin Research Center of AgriculturalBio-technology, Tianjin 300192, China;

    3Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Beijing 100101, China
  • Received:2010-10-08 Revised:2011-01-21 Online:2011-05-01 Published:2011-05-26
  • Contact: Jingan Wang,Zhongqi Liu

摘要: 以受体杨树107和转基因杨树18-1的一年生枝条为材料, 采用Hoagland营养液水培方法, 添加不同浓度的NaCl, 检测二者植株生长以及叶中Na+和K+含量的变化。结果表明, 在0.6%NaCl处理下18-1生根率明显高于107, 生物量较大, 叶片中Na+积累量是107的1.45倍左右。以107和18-1离体叶片为材料, NaCl处理下测定其生理活性指标, 发现18-1叶盘的失绿速度和相对电导率都显著低于107叶盘。把离体叶片接种在改良MS培养基上, 1.2%NaCl处理可使107叶盘几乎停止伸长, 细胞大小不再增加; 而18-1叶盘培养7天后伸长了近50%。以上结果表明利用离体叶片可以鉴定出不同基因型杨树的耐盐潜力。

Abstract: Plant dry weight and changes in Na+ and K+ contents in leaves were investigated in 1-year-old cuttings from the receptor poplar 107 (Populus × euramericana ‘Neva’) and transgenic poplar 18-1 after cuttings were cultured in Hoagland solution with different NaCl concentrations. The growth and rooted percentage of 18-1 was higher than that of 107 with 0.6%NaCl, and the accumulation of Na+ in 18-1 leaves was about 1.45 times higher than that in 107. Physiological characteristics in leaf sections from 107 and 18-1 under NaCl stress were determined. Leaf sections from 18-1 displayed lower chlorophyll decreasing speed and relative electrical conductivity than that from 107. When sections of young poplar leaves were plated on MS medium with 1.2%NaCl, leaf sections from 107 stopped growing, but those from 18-1 grew continuously and their length increased nearly 50% after 7 days. Thus, salt tolerance between different genotypes can be identified in leaf sections.