植物学报 ›› 2011, Vol. 46 ›› Issue (3): 293-301.DOI: 10.3724/SP.J.1259.2011.00293

• 研究报告 • 上一篇    下一篇

转BADH基因紫花苜蓿山苜2号品种的抗盐性鉴定及系统选育

燕丽萍1,2, 夏阳1,2*, 毛秀红1,2, 刘翠兰1,2, 梁慧敏3   

  1. 1山东省林业科学研究院, 济南 250014;
    2山东省林木遗传改良重点实验室, 济南 250014;
    3江苏农林职业技术学院, 句容 212400
  • 收稿日期:2010-10-05 修回日期:2011-01-31 出版日期:2011-05-01 发布日期:2011-05-26
  • 通讯作者: 夏阳
  • 基金资助:

    山东省良种工程

Breeding and Salt Resistance Evaluation of BADH Transgenic Alfalfa Cultivar Shanmu 2

Liping Yan1,2, Yang Xia1,2*, Xiuhong Mao1,2, Cuilan Liu1,2, Huimin Liang3   

  1. 1Shandong Provincial Academy of Forestry, Jinan 250014, China;

    2Shandong Provincial Key Laboratory of Forest Tree GeneticImprovement, Jinan 250014, China;

    3Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, China
  • Received:2010-10-05 Revised:2011-01-31 Online:2011-05-01 Published:2011-05-26
  • Contact: Yang Xia

摘要: 利用转基因技术创造苜蓿新种质已成为牧草新技术育种的重要组成部分。为了有效地从苜蓿转基因植株及其后代中选育出优良品种, 深入研究转基因苜蓿的植物学性状及其产量十分重要。以通过农杆菌介导技术获得的T0代转BADH基因苜蓿为试材, 利用分子生物学方法对其自交株系的世代群体连续进行抗盐性鉴定筛选和系统选育, 首次获得了具有抗盐碱能力的转基因苜蓿稳定株系。同时, 通过品种比较实验、区域实验和生产实验, 表明在不同盐碱地条件下, 转BADH基因的苜蓿植株产草量明显高于对照(未转基因的中苜1号), 生产实验的干草增产率介于13.11%–24.98%之间。上述结果表明, 外源目的基因主要特性的遗传稳定, 进而从实践上验证了转BADH基因工程操作的实用性。

Abstract: Generating new germplasm of alfalfa by transgenic technology has become important in forage breeding. Using a T0 generation of transgenic plants with betaine aldehyde dehydrogenase (BADH) gene as testing material, we tested the resistance to salt. The transgenic grass biomass was higher than that of the non-transgenic control in different salt soils. Dry grass production was higher by 13.11% to 24.98% in the transgenic cultivar than in the control. The betaine aldehyde dehydrogenase gene was stably expressed and inherited, suggesting that this transgenic line can be used for further breeding.

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