Agrobacterium-mediated High-efficient Transformation of Eucalyptus grandis Clone Eg5

doi:10.3724/SP.J.1259.2013.00087

Abstract

Abstract: In this study, we used Eucalyptus grandis clone Eg5 leaves as explants to study the influence of infection time, co-culture pH values and co-culture time on transient expression efficiency. We analyzed the effects of different selection strategies on transgenic plants. Infection time of 45 min, co-culture pH of 5.8, and co-culture time of 3 days achieved the best transient expression efficiency. Screening transgenic plantlets by gradually improving kanamycin concentration increased the selection efficiency to 15% and transformation efficiency was 0.26%. The plantlets were confirmed by GUS staining and PCR analysis.

Key words: Agrobacterium tumefaciens, Eucalyptus grandis, genetic transformation, selection strategies

CLC Number:

S792.39 Q943.2

Lijun Guo, Bingshan Zeng, Ying Liu. Agrobacterium-mediated High-efficient Transformation of Eucalyptus grandis Clone Eg5[J]. Chinese Bulletin of Botany, DOI: 10.3724/SP.J.1259.2013.00087.

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2013.00087

https://www.chinbullbotany.com/EN/Y2013/V48/I1/87

References

参考文献:
Aggarwal, D., A. Kumar,M. Sudhakara Reddy (2011). Agrobacterium tumefaciens mediated genetic transformation of selected elite clone(s) of Eucalyptus tereticornis. Acta Physiologiae Plantarum, 33, 1603.
Alcantara, G. B. D., J. C. Bespalhok Filho,M. Quoirin (2011). Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla . Scientia Agricola, 68, 246-251.
Cervera, M., J. A. Pina, J. Juarez, L. Navarro,L. Pena (1998). Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration. Plant Cell Reports, 18, 271-278.
Da Silva, A. L. L., Y. de Oliveira,J. Da Luz Costa (2011). Preliminary results for genetic transformation of shoot tip of Eucalyptus saligna Sm. via Agrobacterium tumefaciens. Journal of Biotechnology and Biodiversity, 2, 1-6.
Fillatti, J. A. J., J. Sellmer, B. McCown, B. Haissig,L. Comai (1987). Agrobacterium mediated transformation and regeneration of Populus. Molecular and General Genetics MGG, 206, 192-199.
González, E. R., A. de Andrade, A. L. Bertolo, G. C. Lacerda, R. T. Carneiro, V. A. P. Defávari, M. T. V. Labate,C. A. Labate (2002). Production of transgenic Eucalyptus grandis x E. urophylla using the sonication-assisted Agrobacterium transformation (SAAT) system. Functional plant biology, 29, 97-102.
Ho, C. K., S. H. Chang, J. Y. Tsay, C. J. Tsai, V. L. Chiang,Z. Z. Chen (1998). Agrobacterium tumefaciens-mediated transformation of Eucalyptus camaldulensis and production of transgenic plants. Plant Cell Reports, 17, 675-680.
Jefferson, R. A., T. A. Kavanagh,M. W. Bevan (1987). GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO journal, 6, 3901-3907.
J. L. Yao ,K. Lin-Wang. (2004). Eucalyptus transformation method. USA patent. PCT/NZ2004/000242. 2005-04-14.
Joyce, P., M. Kuwahata, N. Turner,P. Lakshmanan (2010). Selection system and co-cultivation medium are important determinants of Agrobacterium-mediated transformation of sugarcane. Plant Cell Reports, 29, 173-183.
Kajita, S., K. Osakabe, Y. Katayama, S. Kawai, Y. Matsumoto, K. Hata,N. Morohoshi (1994). Agrobacterium-mediated transformation of poplar using a disarmed binary vector and the overexpression of a specific member of a family of poplar peroxidase genes in transgenic poplar cell. Plant Science, 103, 231-239.
Mohri, T., N. Yamamoto,K. Shinohara (1996). Agrobacterium-mediated transformation of lombardy poplar (Populus nigra L. var. italica Koehne) using stem segments. Journal of Forest Research, 1, 13-16.
Mullins, K. V., D. J. Llewellyn, V. J. Hartney, S. Strauss,E. S. Dennis (1997). Regeneration and transformation of Eucalyptus camaldulensis. Plant Cell Reports, 16, 787-791.
Oliveira, Y., L. Adamuchio, J. Degenhardt-Goldbach, I. Gerhardt, J. Bespalhok, R. Dibax,M. Quoirin. 2011. Use of kanamycin for selection of Eucalyptus saligna genetically transformed plants., 147-148.: BioMed Central Ltd.
Padilla, I.,L. Burgos (2010). Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols. Plant Cell Reports, 29, 1203-1213.
Petri, C., S. López-Noguera, N. Alburquerque, J. Egea,L. Burgos (2008). An antibiotic-based selection strategy to regenerate transformed plants from apricot leaves with high efficiency. Plant Science, 175, 777-783.
Prakash, M.,K. Gurumurthi (2009). Genetic transformation and regeneration of transgenic plants from precultured cotyledon and hypocotyl explants of Eucalyptus tereticornis Sm. using Agrobacterium tumefaciens. In Vitro Cellular & Developmental Biology - Plant, 45, 429-434.
Spokevicius, A. V., K. Van Beveren, M. A. Leitch,G. Bossinger (2005). Agrobacterium-mediated in vitro transformation of wood-producing stem segments in eucalypts. Plant cell reports, 23, 617-624.
Thakur, A. K., S. Sharma,D. K. Srivastava (2005). Plant regeneration and genetic transformation studies in petiole tissue of Himalayan poplar (Populus ciliata Wall.). Current Science, 89, 664-668.
Wei, T. (2000). Genetic transformation of loblolly pine using mature zygotic embryo expiants by Agrobacterium tumefaciens. Journal of Forestry Research, 11, 215-222.
Wei, T. (2001). Agrobacterium-mediated transformation and assessment of factors influencing transgene expression in loblolly pine (Pinus taeda L.). Cell Research, 11, 237-243.
樊军锋 (2002). mtlD/gutD 双价耐盐基因转化杨树, 猕猴桃的研究. 博士论文. 杨凌:西北农林科技大学. pp.57-59.
方中达 (1979). 植病研究方法. 北京:农业出版社. pp. 1-12.
郭利军, 曾炳山, 刘英, 李湘阳,裘珍飞 (2012). 巨桉无性系Eg5的卡那霉素和头孢霉素敏感性研究. 中南林业科技大学学报, 32, 75-80.
姜清彬 (2011). 细枝木麻黄再生体系及农杆菌介导遗传转化研究. 博士论文. 北京:中国林业科学研究院. pp.73-74.
林平 (2001) 永安桉树造林及其冻害情况调查. 桉树科技, 17, 15-20.
祁述雄 (2002). 中国桉树 (第2版). 北京:中国林业出版社. pp. 228-230.
曾炳山, 张剑, 李建娟, 刘英, 陈李花,范春节 (2010). 桉树人工林冰雪灾害恢复的调查. 中南林业科技大学学报, 30, 69-74.

[1]	Jingjing Li, Yanfei Li, Anqi Wang, Jiaying Wang, Chengyan Deng, Min Lu, Jianying Ma, Silan Dai. Establishment of Regeneration and Genetic Transformation System for Chrysanthemum Cultivar ‘Wandai Fengguang’ [J]. Chinese Bulletin of Botany, 2025, 60(4): 1-0.
[2]	Zeng Wendan, Yan Huabing, Wu Zhengdan, Shang Xiaohong, Cao Sheng, Lu Liuying, Xiao Liang, Shi Pingli, Cheng Dong, Long Ziyuan, Li Jieyu. Agrobacterium rhizogenes-mediated Transformation System of Pueraria lobata Hairy Roots [J]. Chinese Bulletin of Botany, 2025, 60(3): 425-434.
[3]	Yuchen Li, Haixia Zhao, Xiping Jiang, Xintian Huang, Yaling Liu, Zhenying Wu, Yan Zhao, Chunxiang Fu. Establishment of Agrobacterium-mediated Transformation System for Agropyron mongolicum [J]. Chinese Bulletin of Botany, 2024, 59(4): 600-612.
[4]	Yu Xiaomin, Wang Yaqin, Liu Yuhan, Yi Qingping, Cheng Wenhan, Zhu Yu, Duan Feng, Zhang Lixue, He Yanhong. Establishment of Agrobacterium tumefaciens-mediated Genetic Transformation System of Marigold (Tagetes erecta) [J]. Chinese Bulletin of Botany, 2023, 58(5): 760-769.
[5]	Lan Yang, Ya Liu, Yang Xiang, Xiujuan Sun, Jingwei Yan, Aying Zhang. Establishment and Optimization of a Shoot Tip-based Genetic Transformation System for Foxtail Millet [J]. Chinese Bulletin of Botany, 2021, 56(1): 71-79.
[6]	Junhua Li,Shiyu Liu,Chenglong Li,Linlin Han,Yahui Dong,Xiaoli Zhang,Xiting Zhao,Mingjun Li. Establishment of a Genetic Transformation System for Dioscorea opposita Using Microtuber [J]. Chinese Bulletin of Botany, 2019, 54(1): 72-80.
[7]	LIU Yang,ZHANG Jian,CHEN Ya-Mei,CHEN Lei,LIU Qiang. Effect of nitrogen and phosphorus fertilization on biomass allocation and C:N:P stoichiometric characteristics of Eucalyptus grandis seedlings [J]. Chin J Plant Ecol, 2013, 37(10): 933-941.
[8]	Guimei Cui, Yi Sun, Yaoshan Hao, Jianzhong Du, Yixue Wang. The Improvement of Maize Pollen In Vitro Germination Method and Its Role in Pollen-mediated Plant Genetic Transformation [J]. Chinese Bulletin of Botany, 2012, 47(2): 155-161.
[9]	Ying Jiang, Xiuming Liu, Jisheng Ma, Wei Li, Hailin Zhu, Meili Du. Expression of Keratinocyte Growth Factor-1 (KGF-1) in Transgenic Safflower [J]. Chinese Bulletin of Botany, 2011, 46(3): 311-318.
[10]	Xuanyu Liu, Qingyun Wang, Shujun Liu, Songquan Song. Advances in the Genetic Transformation of Sorghum bicolor [J]. Chinese Bulletin of Botany, 2011, 46(2): 216-223.
[11]	Hongbing Fu;Chongshi Cui;Xi Zhao;Qi Liu. Establishment of Cucurbita moschata Genetic Transformation System by Agrobacterium tumefaciens Transfection [J]. Chinese Bulletin of Botany, 2010, 45(04): 472-478.
[12]	Yuanwei Fan;Aizhi Liu;Huafang Wang. Transformation of Populus euphratica [J]. Chinese Bulletin of Botany, 2009, 44(06): 728-734.
[13]	Daojie Wang, Cuiling Yang, Ming Lu. Transformation of Brassica napus by Vacuum Infiltration [J]. Chinese Bulletin of Botany, 2009, 44(02): 216-222.
[14]	Zhi Zou;Changming Lu*. Research in In Planta Transformation Methods and Their Applications and Prospects in Brassica napus [J]. Chinese Bulletin of Botany, 2009, 44(02): 236-244.
[15]	Kaifa Wei*;Yiping Liu;Ziying Lin;Yafang Yang;Zehong Zhang;Wensuo Jia. Problems and Solutions in Agrobacterium tumefaciens-mediated Genetic Transformation of Monocotyledons [J]. Chinese Bulletin of Botany, 2008, 25(04): 491-496.