The Effect of Phenol Concentration on Photosynthetic Physiological Parameters of Salix babylonica

doi:10.11983/CBB15028

Abstract

Abstract: To explore the phenol tolerance of Salix babylonica and the feasibility of its remediation of phenol pollution, we determined the effect of phenol stress on photosynthetic physiology of S. babylonica and its limit mechanism, photosynthesis and chlorophyll fluorescence parameters of cut seedlings of S. babylonica in five phenol concentrations (50, 100, 200, 400 and 800 mg·L^-1) using hydroponics. Phenol inhibited the photosynthesis of S. babylonica significantly, which presented as significantly decreased net photosynthesis rate (P_n), maximum net photosynthesis rate (P_nmax), photosynthetic quantum efficiency, maximum quantum yield of PS II (F_v/F_m) and actual photochemical efficiency (Ф_PSII). The higher the concentration of phenol, the greater the photosynthetic inhibition. Non-stomatal limitation was responsible for reduced net photosynthesis rate (P_n). When S. babylonica was used to phytoremediate the water environment, the concentration of phenol should be < 200 mg·L^-1. Otherwise the efficiency of photosynthesis of S. babylonica was markedly decreased. The limit of phenol concentration that photosynthetic physiological activity of S. babylonica can tolerate needs further experimental study.

Key words: photosynthesis, phenol stress, environmental remediation, Salix babylonica, rectangular hyperbola model

Hui Li, Guangcan Zhang, Huicheng Xie, Jingwei Xu, Chuanrong Li, Juwen Sun. The Effect of Phenol Concentration on Photosynthetic Physiological Parameters of Salix babylonica[J]. Chinese Bulletin of Botany, 2016, 51(1): 31-39.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB15028

https://www.chinbullbotany.com/EN/Y2016/V51/I1/31

Figures/Tables 4

Table 1 The model fitted values of photosynthesis-light response parameters of Salix babylonica

Light response model	Phenol concentration (mg·L^-1)	Φ₀	Φ_c	Φ_c0	LSP	P_nmax	LCP	R_d	R²
Rectangular hyperbolic modified model	0	0.0146	0.0138	0.0135	1192	6.25	9.76	0.132	0.994
	50.0	0.0134	0.0130	0.0128	1002	3.91	20.31	0.261	0.995
	100.0	0.0128	0.0124	0.0121	976	3.36	27.26	0.329	0.998
	200.0	0.0122	0.0109	0.0101	763	2.88	49.60	0.501	0.997
	400.0	0.0115	0.0102	0.0099	601	1.94	68.38	0.680	0.993
	800.0	-	-	-	-	-	-	-	-

Figure 2 The response of photosynthetic physiological parameters of Salix babylonica to phenol concentration under the same photosynthetically active radiation (1 200 μmol·m-2·s-1) (means±SD)Different lowercase letters mean significant difference among treatments at 0.05 level. Pn: Net photosynthetic rate; Tr: Transpiration rate; Gs: Stomatal conductance; Ci: Intercellular CO2 concentration; Ls: Stomatal limitation; WUE: Water use efficiency

Figure 3 Effect of phenol concentration on chlorophyll fluorescence parameters (Fv/Fm (A), 1-qP (B), NPQ (C) and ΦPSII (D)) of Salix babylonica (means±SD)Different lowercase letters in the same column mean significant difference among treatments at 0.05 level. Fv/Fm: Maximal quantum yield of PSII photochemistry; qP: Photochemical quenching coefficient; NPQ: Nonphotochemical quenching; ΦPSII: Effective quantum yield of PSII photochemistry

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