Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (1): 90-95.DOI: 10.11983/CBB19168

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Optimization of Cell Suspension Culture Conditions of Achyranthes bidentata

Ping Li1,2,Yahui Dong1,Chenglong Li1,Yulong He1,Mingjun Li1,3,*()   

  1. 1College of Life Science, Henan Normal University, Xinxiang 453007, China
    2Zhengzhou Shuqing Medical College, Zhengzhou 450064, China
    3Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province/Engineering Laboratory of Biotechnology for Green Medicinal Plant of Henan Province, Xinxiang 453007, China
  • Received:2019-08-27 Accepted:2019-11-13 Online:2020-01-01 Published:2019-12-20
  • Contact: Mingjun Li

Abstract: To optimize the suspension culture conditions of Achyranthes bidentata cells, effect of different factors like inoculum concentrations, subculture cycles, pH, light and Cu 2+ on cell growth and polysaccharides contents was studied. The results showed that, cells grew well with a good ability for polysaccharides synthesis under 50 g·L -1 inoculum concentration, 14 d subculture cycle, pH5-6 and light. In the presence of 50 μmol·L -1 Cu 2+, the cell dry weight could be the maximum with 44.63 g·L -1, and polysaccharides contents reached the peak at 4.02 mg·g -1.

Key words: Achyranthes bidentata, cell suspension culture, cell growth, polysaccharides