Differences in Photosynthetic Pigments and Photosynthetic Enzyme Activities in Different Internodes of Phyllostachys edulis During Rapid Growth Stage

doi:10.11983/CBB17195

Abstract

Abstract: This study aimed to reveal the characteristics of photosynthetic carbon assimilation and the change regulation in different internodes of Phyllostachys edulis stems during their rapid growth stage. We analyzed the photosynthetic pigment content and activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), phosphoenolpyruvate carboxylase (PEPC), NADP-malate dehydrogenase (NADP-MDH), NADP-malic enzyme (NADP-ME), phosphoenolpyruvate carboxykinase (PEPCK) and pyruvate, and orthophosphate dikinase (PPDK) in stems with different internodes. With increasing internode number of P. edulis stems, the content of chlorophyll a, chlorophyll b and carotenoid was all significantly reduced and the ratio of chlorophyll a/b showed a gradual upward trend. The activities of Rubisco, PEPC and PPDK in stems of P. edulis decreased significantly from the 1^st to the 10^th internode, then the rate of decrease gradually slowed down. The activities of NADP-ME declined significantly from the 1^st to the 13^th internode; after the 13^th internode, the enzyme activities tended to be stable. The activities of NADP-MDH decreased markedly from 1^st to the 25^th internode of stems. The ratio of PEPC/Rubisco increased gradually and the range was between 18.37 and 65.09, which was significantly larger than that in typical C₃ plants. The research showed that the assimilating capacity with carbon differed in different internodes, and the middle and lower stems grew relatively fast. The result of many C₄ enzymes and activities being significantly high provided strong evidence for the existence of a C₄ photosynthetic pathway in the stems of P. edulis.

Key words: chlorophyll, C₄ pathway, photosynthetic enzyme, Phyllostachys edulis, internode

Sun Jianfei, Zhai Jianyun, Ma Yuandan, , Fu Lucheng, Bu Keli, Wang Keyang, Gao Yan, Zhang Rumin. Differences in Photosynthetic Pigments and Photosynthetic Enzyme Activities in Different Internodes of Phyllostachys edulis During Rapid Growth Stage[J]. Chinese Bulletin of Botany, 2018, 53(6): 773-781.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB17195

https://www.chinbullbotany.com/EN/Y2018/V53/I6/773

Figures/Tables 4

Table 1 Differences of pigment contents in the Phyllostachys edulis stems of different internodes (means±SD)

Internode number	Chlorophyll a (μg·g^-1FW)	Chlorophyll b (μg·g^-1FW)	Total chlorophyll (μg·g^-1FW)	Carotenoids (μg·g^-1FW)	Chlorophyll a/b
1	15.19±0.28 a	5.07±0.04 a	20.26±0.29 a	6.37±0.27 a	3.00±0.24 c
4	16.22±0.27 a	5.27±0.40 a	21.49±0.39 a	7.00±0.28 a	3.10±0.27 c
7	15.80±1.09 a	5.21±0.20 a	21.01±1.28 a	6.66±0.48 a	3.03±0.10 c
10	13.98±0.42 b	3.91±0.11 b	17.89±0.53 b	6.27±0.32 a	3.58±0.02 c
13	9.51±0.63 c	2.71±0.03 c	12.22±0.61 c	4.27±0.19 b	3.50±0.25 c
16	6.49±0.29 d	1.70±0.09 d	8.19±0.23 d	2.89±0.17 c	3.83±0.35 bc
19	2.43±0.07 e	0.53±0.02 e	2.96±0.09 e	1.23±0.09 d	4.59±0.1 ab
22	1.83±0.15 e	0.38±0.03 e	2.22±0.16 e	0.97±0.05 d	4.82±0.55 a
25	1.25±0.01 e	0.25±0.01 e	1.50±0.01 e	0.63±0.09 d	5.07±0.23 a

Figure 1 Differences of Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity (A) and Phosphoenolpyruvate carboxylase (PEPC) activity (B) in the Phyllostachys edulis stems of different internodes (means±SD)

Figure 2 Differences of activities of 4 C4 enzymes in the Phyllostachys edulis stems of different internodes (means± SD) (A) NADP-malate dehydrogenase (NADP-MDH) activity and Phosphoenolpyruvate carboxy-kinase (PEPCK) activity; (B) NADP-malic enzyme (NADP-ME) activity; (C) Pyruvate, orthophosphate dikinase (PPDK) activity

Figure 3 Changes of the ratios of Phosphoenolpyruvate carboxylase/Ribulose-1,5-bisphosphate carboxylase/oxygen- ase (PEPC/Rubisco) in the Phyllostachys edulis stems of different internodes (means±SD)

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