Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (4): 479-489.DOI: 10.11983/CBB22066

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Establishment of a Comprehensive Evaluation System for Aluminum Tolerance in Tomato Seedlings Based on Principal Component Analysis-Clustering Analysis-Stepwise Regression Analysis

Guo Shuya1, Ai Jinxiang1, Chen Hongyu1, Shao Yeyao1, Wang Yan1, Wang Qian1, Ye Yitong1, Zhang Yating1, Ding Zhexiao1, Wu Haochen1, Wu Yuhuan2,3, Zhang Jianxin4, Rao Mide1,*(), Liu Peng1,*()   

  1. 1College of Chemistry and Life Sciences, Zhejiang Normal University/Botany Laboratory, Jinhua 321004, China
    2College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
    3Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
    4Lishui Vocational and Technical College, Lishui 323000, China
  • Received:2022-04-08 Revised:2022-05-12 Online:2022-07-01 Published:2022-07-14
  • Contact: Rao Mide,Liu Peng

Abstract: Aluminum toxicity is one of the major factors limiting crop production in acid soils. Tomato is a dominant economic crop in acid soil and different varieties of tomato have different responses to aluminum stress. Therefore, screening aluminum tolerant seedling is of great significance for tomato production and research. In this study, 10 tomato varieties were used as materials, which were cultured indoor in pots containing soil, treated with 1 000 µmol∙L-1 AlCl3·6H2O. 16 morphological, physiological, biochemical or photosynthetic indicators reflecting the growth of plants under aluminum stress were determined. Through principal component analysis, 16 indicators of tomato seedlings under aluminum stress were converted into 5 independent comprehensive indicators, with a cumulative contribution rate of 90.779%. Based on the systematic cluster analysis of aluminum resistance value (A), the test germplasms were divided into five levels, category I is a highly aluminum-tolerant variety, Qianxi, and category V was a highly aluminum-intolerant variety, Puluowangsi. According to the multiple linear regression analysis, 4 of the 16 indicators were found to be significantly correlated with the A value (P<0.01): malondialdehyde content (X3), net photosynthetic rate (X6), leaf area (X10) and dry weight of underground (X15), with the following evaluation equation: y=0.046+0.405X6+0.515X10-0.207X15+0.028X3 (R2=0.997). The evaluation equation can be used to judge the aluminum toxicity resistance characteristics of different tomato varieties in seedling stage, which made the identification of tomato aluminum tolerance quick and simplified.

Key words: Solanum lycopersicum, aluminum stress, germplasm screening, acid soil, comprehensive evaluation