Research Progress of Hyperhydricity Mechanism in Plant Seedling Growth

doi:10.11983/CBB21126

Abstract

Abstract:

Hyperhydricity (HH) or vitrification is a physiological disorder during plant growth, which shows a typical translucent phenotype like water-soaked that often occurs in the plant tissue culture. Vitrification is one of the three main influencing factors that limit the tissue culture technology and the commercialized production of seedlings during the plant tissue culture, but its molecular mechanism is still unclear by far. Using plantlets from the tissue culture as a research object often lead to an interference of man-made factors, which can be avoided by non-tissue culture material to reveal the molecular mechanism of vitrification in nature. This review summarized the recent progress of mechanism for inducing HH in non-tissue culture plants, including the abnormal deposit of suberin, the reduction of cuticular wax, the lipid peroxidation in cellular membranes and the disrupted transmembrane transport of ion or water, to provide new clues and thinking for hyperhydricity in plant seedling growth.

Key words: aquaporins, cell membrane, cuticular wax, hyperhydricity, suberin

Qingping Zhao, Yuping liang, Fangyuan Zhou, Xiang Zhao. Research Progress of Hyperhydricity Mechanism in Plant Seedling Growth[J]. Chinese Bulletin of Botany, 2022, 57(1): 90-97.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB21126

https://www.chinbullbotany.com/EN/Y2022/V57/I1/90

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References 49

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材料类型	基因或蛋白名称	作用	调节玻璃化机制	参考文献
非组培材料	ABCG5	ABC转运蛋白G亚家族成员	通过调节表皮蜡质形成, 使表皮角质层的厚度降低, 渗透率明显增加, 外界水分大量渗入引发玻璃化	Lee et al., 2021
	ACA8	IIB亚家族中的P型自抑制Ca²⁺-ATP酶	主要参与调节胞内Ca²⁺的输出, 维持细胞内的低Ca²⁺水平, 突变后细胞膜完整性受损, 细胞内离子大量泄漏引发玻璃化	Zhang et al., 2014
	CRI1	拟南芥cristal突变体, 功能未知	突变后可能通过促进细胞渗透压升高、细胞分裂素水平增高以及表面的角质层改变导致植物玻璃化	Delarue et al., 1997
	FRO1	编码NADH脱氢酶	编码的NADH脱氢酶是线粒体电子传递链的一个亚基, 突变后导致线粒体内电子传递异常, 活性氧大量积累, 细胞膜的完整性受损, 细胞内离子大量泄漏引发玻璃化	Lee et al., 2002
	GPAT5	甘油-3-磷酸酰基转移酶5	通过影响软木脂及其相关蜡质的合成使植物的渗透率增加, 导致高湿环境中水分大量渗入引发玻璃化	Hsu et al., 2021
	TIP1;1	编码水孔蛋白	超表达后导致叶肉细胞间水分过度积累引发玻璃化	Zhu et al., 2014
	TG	拟南芥ERF家族转录因子	超表达后通过影响水孔蛋白基因TIP1;1功能导致叶肉细胞间水分过度积累引发玻璃化	Zhu et al., 2014
组培材料	ACS1和ACOI	乙烯合成相关基因	基因表达被抑制后通过降低内源乙烯含量间接促进H₂O₂含量降低和气孔开度增加, 促进玻璃化植物的失水和复壮	Sreelekshmi and Siril, 2020
	BiP蛋白同源蛋白	受生理胁迫表达	在植物玻璃化中的作用机制目前还不清楚	Fontes et al., 1999
	PAL	苯丙氨酸解氨酶	突变后降低木质素的合成引发玻璃化	Phan and Hegedus, 1986
	NADPH氧化酶	烟酰胺腺嘌呤二核苷酸磷酸氧化酶	通过诱导活性氧的产生引发玻璃化	Tian et al., 2017

材料类型	基因或蛋白名称	作用	调节玻璃化机制	参考文献
非组培材料	ABCG5	ABC转运蛋白G亚家族成员	通过调节表皮蜡质形成, 使表皮角质层的厚度降低, 渗透率明显增加, 外界水分大量渗入引发玻璃化	Lee et al., 2021
	ACA8	IIB亚家族中的P型自抑制Ca²⁺-ATP酶	主要参与调节胞内Ca²⁺的输出, 维持细胞内的低Ca²⁺水平, 突变后细胞膜完整性受损, 细胞内离子大量泄漏引发玻璃化	Zhang et al., 2014
	CRI1	拟南芥cristal突变体, 功能未知	突变后可能通过促进细胞渗透压升高、细胞分裂素水平增高以及表面的角质层改变导致植物玻璃化	Delarue et al., 1997
	FRO1	编码NADH脱氢酶	编码的NADH脱氢酶是线粒体电子传递链的一个亚基, 突变后导致线粒体内电子传递异常, 活性氧大量积累, 细胞膜的完整性受损, 细胞内离子大量泄漏引发玻璃化	Lee et al., 2002
	GPAT5	甘油-3-磷酸酰基转移酶5	通过影响软木脂及其相关蜡质的合成使植物的渗透率增加, 导致高湿环境中水分大量渗入引发玻璃化	Hsu et al., 2021
	TIP1;1	编码水孔蛋白	超表达后导致叶肉细胞间水分过度积累引发玻璃化	Zhu et al., 2014
	TG	拟南芥ERF家族转录因子	超表达后通过影响水孔蛋白基因TIP1;1功能导致叶肉细胞间水分过度积累引发玻璃化	Zhu et al., 2014
组培材料	ACS1和ACOI	乙烯合成相关基因	基因表达被抑制后通过降低内源乙烯含量间接促进H₂O₂含量降低和气孔开度增加, 促进玻璃化植物的失水和复壮	Sreelekshmi and Siril, 2020
	BiP蛋白同源蛋白	受生理胁迫表达	在植物玻璃化中的作用机制目前还不清楚	Fontes et al., 1999
	PAL	苯丙氨酸解氨酶	突变后降低木质素的合成引发玻璃化	Phan and Hegedus, 1986
	NADPH氧化酶	烟酰胺腺嘌呤二核苷酸磷酸氧化酶	通过诱导活性氧的产生引发玻璃化	Tian et al., 2017