Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (6): 733-739.DOI: 10.11983/CBB20051
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Fanyu Lin, Xijie Yin*(), Yuna Liang, Jiechao Huang
Received:
2020-03-25
Accepted:
2020-07-21
Online:
2020-11-01
Published:
2020-11-11
Contact:
Xijie Yin
Fanyu Lin, Xijie Yin, Yuna Liang, Jiechao Huang. Analysis of In Situ Distribution of Inorganic Elements in Plants by Micro-XRF[J]. Chinese Bulletin of Botany, 2020, 55(6): 733-739.
Vacuum (%) | Vented (%) | |||||||
---|---|---|---|---|---|---|---|---|
Dwell time (ms) | 200 | 50 | 30 | 10 | 200 | 50 | 30 | 10 |
Mg | 4.6 | 4.6 | 4.6 | 4.7 | 4.7 | 5.2 | 5.1 | 4.7 |
Al | 2.3 | 2.4 | 2.3 | 2.3 | 3.6 | 3.1 | 3.6 | 3.9 |
Si | 1.4 | 1.3 | 1.4 | 1.5 | 0.6 | 0.5 | 0.6 | 0.6 |
P | 0.7 | 0.7 | 0.8 | 0.8 | 0.2 | 0.2 | 0.2 | 0.3 |
S | 2.1 | 2.1 | 2.2 | 2.2 | 0.6 | 0.6 | 0.6 | 0.6 |
K | 13.7 | 13.6 | 13.7 | 13.7 | 12.8 | 12.6 | 12.8 | 12.4 |
Ca | 38.2 | 38.9 | 39.3 | 39.4 | 36.5 | 36.5 | 37.2 | 36.2 |
Cr | 3.6 | 3.6 | 3.6 | 3.5 | 3.6 | 3.4 | 3.6 | 3.8 |
Mn | 3.4 | 3.4 | 3.4 | 3.3 | 3.4 | 3.5 | 3.3 | 3.6 |
Fe | 11.8 | 11.6 | 11.2 | 11.5 | 14.4 | 14.6 | 13.6 | 13.4 |
Ni | 1.6 | 1.6 | 1.5 | 1.5 | 2.2 | 2.1 | 2.2 | 2.5 |
Cu | 2.0 | 2.0 | 1.9 | 1.9 | 2.8 | 2.6 | 2.8 | 2.9 |
Zn | 1.6 | 1.6 | 1.5 | 1.5 | 2.1 | 2.1 | 2.0 | 2.4 |
As | 0.5 | 0.4 | 0.4 | 0.4 | 0.8 | 0.5 | 0.6 | 0.7 |
Cd | 9.1 | 9.0 | 9.0 | 8.7 | 6.8 | 7.2 | 6.6 | 7.0 |
Pb | 3.3 | 3.3 | 3.1 | 3.1 | 5.1 | 5.3 | 5.2 | 5.0 |
Table 1 Micro-XRF area scanning results under different conditions
Vacuum (%) | Vented (%) | |||||||
---|---|---|---|---|---|---|---|---|
Dwell time (ms) | 200 | 50 | 30 | 10 | 200 | 50 | 30 | 10 |
Mg | 4.6 | 4.6 | 4.6 | 4.7 | 4.7 | 5.2 | 5.1 | 4.7 |
Al | 2.3 | 2.4 | 2.3 | 2.3 | 3.6 | 3.1 | 3.6 | 3.9 |
Si | 1.4 | 1.3 | 1.4 | 1.5 | 0.6 | 0.5 | 0.6 | 0.6 |
P | 0.7 | 0.7 | 0.8 | 0.8 | 0.2 | 0.2 | 0.2 | 0.3 |
S | 2.1 | 2.1 | 2.2 | 2.2 | 0.6 | 0.6 | 0.6 | 0.6 |
K | 13.7 | 13.6 | 13.7 | 13.7 | 12.8 | 12.6 | 12.8 | 12.4 |
Ca | 38.2 | 38.9 | 39.3 | 39.4 | 36.5 | 36.5 | 37.2 | 36.2 |
Cr | 3.6 | 3.6 | 3.6 | 3.5 | 3.6 | 3.4 | 3.6 | 3.8 |
Mn | 3.4 | 3.4 | 3.4 | 3.3 | 3.4 | 3.5 | 3.3 | 3.6 |
Fe | 11.8 | 11.6 | 11.2 | 11.5 | 14.4 | 14.6 | 13.6 | 13.4 |
Ni | 1.6 | 1.6 | 1.5 | 1.5 | 2.2 | 2.1 | 2.2 | 2.5 |
Cu | 2.0 | 2.0 | 1.9 | 1.9 | 2.8 | 2.6 | 2.8 | 2.9 |
Zn | 1.6 | 1.6 | 1.5 | 1.5 | 2.1 | 2.1 | 2.0 | 2.4 |
As | 0.5 | 0.4 | 0.4 | 0.4 | 0.8 | 0.5 | 0.6 | 0.7 |
Cd | 9.1 | 9.0 | 9.0 | 8.7 | 6.8 | 7.2 | 6.6 | 7.0 |
Pb | 3.3 | 3.3 | 3.1 | 3.1 | 5.1 | 5.3 | 5.2 | 5.0 |
Figure 2 Distribution characteristics of different elements in stem cross section (A) Stem cross section; (B) P; (C) S; (D) Ca; (E) K; (F) Mn; (G) Ca, K, Mn overlay. Bars=500 μm
Figure 3 Distribution characteristics of Mn in leaf (A)-(C) Control group ((A) Leaf; (B) Mn concentration heatmap; (C) Ca/Mn colormap); (D)-(G) Experimental group ((D) Leaf; (E) Mn concentration heatmap; (F) Ca/Mn colormap; (G) Linear distribution of Mn). Bars=5 mm
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