Chinese Bulletin of Botany ›› 2020, Vol. 55 ›› Issue (4): 430-441.DOI: 10.11983/CBB19204

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Proteome Analysis of Different Resistant Apple Cultivars in Response to the Stress of Ring Rot Disease

Caixia Zhang,Gaopeng Yuan,Xiaolei Han,Wuxing Li,Peihua Cong()   

  1. Key Laboratory of Horticulture Crops Germplasm Resources Utilization, Ministry of Agriculture, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China
  • Received:2019-10-18 Accepted:2020-04-26 Online:2020-07-01 Published:2020-05-21
  • Contact: Peihua Cong

Abstract:

The aim of this study was to investigate the differential expression of resistance related proteins in resistant and susceptible apple leaves in response to ring rot disease. The resistant and susceptible apple cultivars Huayue and Golden Delicious were analyzed by high-throughput isobaric tag (IBT) marker quantitative analysis combined with liquid chromatography-tandem mass spectrometry (LC-MS), proteome differential expression of resistant and susceptible apple leaves before and after pathogen treatment was analyzed, and 171 differential expression proteins (DEPs) were identified. GO enrichment and KEGG pathway analysis showed that 686 GO entries were annotated in cell components, molecular functions, and biological processes, of which 52 DEPs were annotated in 18 distinct pathways of KEGG pathway (P<0.05). Subcellular localization prediction analysis showed that 170 DEPs in 171 DEPs were located in 8 organelles. Functional annotation analysis showed that 46 DEPs were annotated in 7 classes of resistance-related proteins, including the thaumatin-like proteins, peroxidases, polyphenol oxidases, Mal d1 proteins, chitinases, endo-1,3-beta-glucosidases and MLP-like proteins. In addition, the expression characteristics and gene quantitative results of resistance-related proteins were analyzed. The results of this study provided a technical reference for further understanding the resistance mechanism of resistant and susceptible apples to the stress of ring rot disease.

Key words: apple, resistant and susceptible, IBT proteomics, ring rot disease, resistance-related proteins