Chinese Bulletin of Botany ›› 2018, Vol. 53 ›› Issue (1): 94-103.DOI: 10.11983/CBB16247

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Complete Chloroplast Genome Sequence and Characteristics Analysis of Morus multicaulis

Qiaoli Li, Na Yan, Qiong Song, Junzhan Guo*()   

  1. College of Forestry, Northwest A & F University, Yangling 712100, China
  • Received:2016-12-13 Accepted:2017-03-29 Online:2018-01-01 Published:2018-08-10
  • Contact: Junzhan Guo

Abstract:

Mulberry is an economically important crop in Asia. We determined the complete chloroplast sequence of cultivated species of Morus multicaulis. Ribenhuchen was used as experimental material. High-throughput sequencing was used to sequence the chloroplast genome and the genome structure (NCBI No.: KU355297), and we compared the chloroplast genome with those of reported sibling species (Morus mongolica, M. indica, M. notabilis). The chloroplast genome (cpDNA) of M. multicaulis with a typical quadripartite structure is 159 154 bp long. The cpDNA of M. multicaulis contains 130 genes, including 85 protein coding genes (18 genes duplicated in the inverted repeat regions), 37 transfer RNA genes and 8 ribosomal RNA genes. There are 82 simple sequence repeats, and the number of mono-, di-, tri-, tetra-, pentanucleotide repeat motifs is 63, 7, 2, 9, and 1, with no hexanucleotide repeat sequences. Mono-nucleotide repeat sequences accounted for 76.8% of the cpDNA of simple sequence repeats. MEGA 6.0 was used to construct the phylogenetic tree of 15 species and for cluster analysis of Morus plants. M. multicaulis and M. mongolica were clustered into one group. The research results have reference value for chloroplast genome research, molecular marker development and breeding of mulberry.

Key words: Morus multicaulis, chloroplast genome, high-throughput sequencing, cluster analysis